| Abstract | 第4页 |
| 摘要 | 第5-23页 |
| Chapter 1 Review of Relevant Literature | 第23-43页 |
| 1.1 Cardiovascular diseases | 第23-28页 |
| 1.1.1 General overview of the global impact of coronary heart disease andother cardiovascular diseases | 第23页 |
| 1.1.2 CHD and other cardiovascular and circulatory diseases burden in China | 第23-24页 |
| 1.1.3 Causes or risk factors associated with CHD | 第24-28页 |
| 1.2 AdipoQ(adiponectin,C1Q and collagen domain containing) | 第28-31页 |
| 1.2.1 Overview and structure of AdipoQ | 第28-29页 |
| 1.2.2 Association of AdipoQ SNPs with CVDs | 第29-31页 |
| 1.3 Adiponectin | 第31-35页 |
| 1.3.1 Adiponectin discovery,structure and forms | 第31-32页 |
| 1.3.2 Functions of adiponectin | 第32-34页 |
| 1.3.3 Association of adiponectin with AdipoQ polymorphisms | 第34页 |
| 1.3.4 Association of adiponectin with CVDs | 第34-35页 |
| 1.4 MicroRNA(miRNA)target prediction and application in CAD diagnosis | 第35-38页 |
| 1.5 Meta-analysis | 第38-40页 |
| 1.6 Research main objectives | 第40-43页 |
| Chapter 2 Methods and Materials | 第43-51页 |
| 2.1 A meta-analysis on the association between CVD and single nucleotidevariants of AdipoQ | 第43-46页 |
| 2.1.1 Search strategy | 第43页 |
| 2.1.2 Inclusion and exclusion criteria | 第43-44页 |
| 2.1.3 Assessment of study quality | 第44页 |
| 2.1.4 Data extraction | 第44页 |
| 2.1.5 Statistical analysis | 第44-46页 |
| 2.2 Association studies | 第46-50页 |
| 2.2.1 Study participants | 第46页 |
| 2.2.2 Ethical statement | 第46页 |
| 2.2.3 Epidemiological survey and biochemical examination | 第46-47页 |
| 2.2.4 SNP selection | 第47页 |
| 2.2.5 DNA extraction | 第47页 |
| 2.2.6 SNP genotyping | 第47-48页 |
| 2.2.7 Adiponectin measurement | 第48-49页 |
| 2.2.8 Statistical analyses | 第49-50页 |
| 2.3 MicroRNA target prediction | 第50-51页 |
| 2.3.1 Sequence used | 第50页 |
| 2.3.2 Databases searched | 第50页 |
| 2.3.3 Search Procedure | 第50-51页 |
| Chapter 3 Results | 第51-131页 |
| 3.1 Results of meta-analysis | 第51-105页 |
| 3.1.1 Overall results | 第51-56页 |
| 3.1.2 Meta-analysis for the rs266729(-11377C>G)variant | 第56-73页 |
| 3.1.3 Meta-analysis for rs2241766(+45T>G)polymorphism | 第73-90页 |
| 3.1.4 Meta-analysis for rs1501299(+276 G>T)polymorphism | 第90-105页 |
| 3.2 Association between Coronary Heart Disease(CHD)and the rs3774261,rs1063537 and rs2082940 variants of the AdipoQ gene | 第105-124页 |
| 3.2.1 Demographics and lipid profile of participants | 第105-106页 |
| 3.2.2 SNPs information | 第106-111页 |
| 3.2.3 Lipid profiles in different SNP genotypes and alleles in controls | 第111-112页 |
| 3.2.4 Participants' genotypes distributions and allelic frequencies | 第112页 |
| 3.2.5 Associations between AdipoQ gene polymorphisms and CHD risk | 第112-114页 |
| 3.2.6 Pairwise linkage disequilibrium among the three SNPs | 第114-115页 |
| 3.2.7 Haplotypes of AdipoQ Gene with the risk of CHD | 第115-116页 |
| 3.2.8 CHD risk associated with smoking and drinking | 第116页 |
| 3.2.9 Interaction between AdipoQ SNPs and environmental factors in CHD 94 | 第116-119页 |
| 3.2.10 Distribution of adiponectin levels (μg/ml) by gender and age in cases and controls | 第119-120页 |
| 3.2.11 Comparison of plasma adiponectin levels (μg/ml) between CHD cases | 第120-121页 |
| 3.2.12 Distribution of adiponectin levels by genotypes | 第121-122页 |
| 3.2.13 Comparisons of adiponectin levels by genotypes in cases and control | 第122-124页 |
| 3.3 MicroRNA Target prediction | 第124-131页 |
| 3.3.1. Prediction results from the miRGator database | 第124页 |
| 3.3.2 Prediction results from the miRDB database | 第124-127页 |
| 3.3.3 Prediction results from the RegRNA database | 第127-131页 |
| Chapter 4 Discussion | 第131-145页 |
| 4.1 Meta-analysis on the association between CVD risk and single nucleotidevariants of AdipoQ | 第131-135页 |
| 4.2 Association between CHD and the rs3774261, rs1063537 and rs2082940variants of AdipoQ | 第135-137页 |
| 4.3 Gene-environment interactions in CHD | 第137-139页 |
| 4.4 Association between plasma levels of adiponectin and AdipoQ variants,CHD, gender and age | 第139-140页 |
| 4.5 MicroRNA target prediction | 第140-142页 |
| 4.6 Study Limitations | 第142-143页 |
| 4.7 Study novelty | 第143-145页 |
| Chapter 5 Conclusions | 第145-147页 |
| References | 第147-167页 |
| Short introduction of author | 第167-171页 |
| Acknowledgement | 第171-172页 |
