| Content | 第6-8页 |
| 英文摘要 | 第8页 |
| 1． Introduction | 第10-11页 |
| 2． Literature Review | 第11-20页 |
| 2．1． Microarray | 第11-14页 |
| 2．1．1 Developing | 第11-12页 |
| 2．1．2 Application | 第12页 |
| 2．1．3 Advantages and disadvantages | 第12-13页 |
| 2．1．4 Perspective | 第13-14页 |
| 2．2． General aspects about Abscisic acid （ABA） | 第14-20页 |
| 2．2．1 Abscisic acid functions and mechanisms | 第14-16页 |
| 2．2．2 Abscisic acid signal transduction research | 第16-17页 |
| 2．2．3 Abscisic acid in environmental stress condition | 第17-19页 |
| 2．2．4 Perspective | 第19-20页 |
| 3． Materials and Methods | 第20-32页 |
| 3．1． Preparation of rice samples | 第20页 |
| 3．1．1 ABA treatment and sampling of three rice lines | 第20页 |
| 3．1．2 Seedling growing of Zhongzao 21 andsampling of different tissue at different development stage | 第20页 |
| 3．2 Total RNA isolation | 第20-21页 |
| 3．3 RNA electrophoresis analysis | 第21页 |
| 3．4 Isolation of Poly A mRNA from total RNA | 第21-22页 |
| 3．5． Preparation of total cDNA probes | 第22页 |
| 3．6 cDNA Microarray preparation | 第22-23页 |
| 3．6．1． cDNA library plating | 第22-23页 |
| 3．6．2． Clones Inoculation | 第23页 |
| 3．6．3． Plasmid Store （LB/glycerol 1:1） | 第23页 |
| 3．7． Plasmid extraction by Minipreparation using MultiScreen 96-Well Filter plates | 第23-26页 |
| 3．7．1． Sequencing and analysis | 第25页 |
| 3．7．2． Polymerase Chain Reaction （PCR） | 第25页 |
| 3．7．3． PCR product cleaning | 第25页 |
| 3．7．4． Database building | 第25-26页 |
| 3．7．5． Templete Dilution and Polymerase Chain Reaction （PCR） | 第26页 |
| 3．7．6． PCR product precipitation | 第26页 |
| 3．7．7． PCR product denaturation | 第26页 |
| 3．8． Preparation of Array | 第26-27页 |
| 3．9 Chip hybridization | 第27-29页 |
| 3．9．1． Prehybridization | 第27-28页 |
| 3．9．2． Hybridization | 第28页 |
| 3．9．3． Membranes washing | 第28-29页 |
| 3．9．4． X film autoradioactivity exposure | 第29页 |
| 3．9．5． X-film Analysis and differential display gene search | 第29页 |
| 3．10． Reverse Northern． | 第29-30页 |
| 3．11 Northern blotting | 第30-31页 |
| 3．12 Full-length sequence and bioinformatics analysis | 第31-32页 |
| 4． Results and Analysis． | 第32-53页 |
| 4．1． cDNA microarray analysis of tissues-specific genes in rice | 第32-42页 |
| 4．1．1 Preparation of PCR products of 1330 unigenes and micro-arraying process | 第32-33页 |
| 4．1．2 Isolation of total RNA from rice different tissues of root, stem and leaf, and synthesis of cDNA first-chain probes | 第33-34页 |
| 4．1．3 Identification of differentially expressed genes in rice different tissues （root, stem and leaf） | 第34-35页 |
| 4．1．4 Northern blotting: to test the expression levelof H012d03 clone in different tissues of rice | 第35-36页 |
| 4．1．5 DNA full sequence analysis of H012d03 clone | 第36页 |
| 4．1．6 the electronic PCR location and bioinformatics analysis of H012d03 clone | 第36-42页 |
| 4．2 cDNA microarray detection of mRNA expression of 4370 genes in rice induced by phytohormone ABA． | 第42-53页 |
| 4．2．1 cDNA chip preparation | 第42页 |
| 4．2．2 Isolation of total RNA from rice stem treated with ABA and the synthesis of cDNA probes | 第42-44页 |
| 4．2．3 The bioassay of fluctuation of gene expression in rice stem tissue induced by phytohormone ABA． | 第44-45页 |
| 4．2．4 Data of a number of positive spots obtained through chip hybridization | 第45页 |
| 4．2．5 Using reverse northern blot to confirm the expression situation of the 5 cDNA clones inhibited by ABA treatment | 第45-46页 |
| 4．2．6 Bioinformatics analysis of Cytochrome C clone． | 第46-53页 |
| 5． Discussion | 第53-55页 |
| 5．1 cDNA microarray could be used practically for monitoring the expression level of thousands of genes | 第53-54页 |
| 5．2 A repeated gene that could transcribed into RNA with polyA tail also existed in the structural gene of 23S rRNA in ricechloroplast genome | 第54-55页 |
| 5．3 Cytochrome C gene may play a role in stress response induced by ABA | 第55页 |
| Conclusion: | 第55-56页 |
| Reference | 第56-57页 |
