| Abstract in Chinese | 第8-10页 |
| Abstract in English | 第10-12页 |
| 1 Introduction | 第13-26页 |
| 1.1 The Purpose and Significance of Study | 第13-14页 |
| 1.2 Literature Review | 第14-24页 |
| 1.2.1 Role of Myo-inositol and Other Derivatives in Plant Growth and Development | 第15-17页 |
| 1.2.2 Role of MIPS Family Genes in Biotic & Abiotic Stresses | 第17-18页 |
| 1.2.3 Mutations in MIPS Protein and its Consequences | 第18-19页 |
| 1.2.4 SNARE Protein Family: Structure and Housekeeping Functions | 第19-20页 |
| 1.2.5 t-SNAREs Super family in Plants | 第20-21页 |
| 1.2.6 Role of SNAP Proteins in Plant Growth and Development | 第21-24页 |
| 1.3 Main Contents and Technical Plan.of Study | 第24-25页 |
| 1.3.1 The Innovation of Study | 第25页 |
| 1.4 Objectives of Work | 第25-26页 |
| 2 Materials and Methods | 第26-36页 |
| 2.1 Materials | 第26-27页 |
| 2.1.1 Plant Materials | 第26页 |
| 2.1.2 Database and Software | 第26页 |
| 2.1.3 Strains and Plasmids | 第26页 |
| 2.1.4 Reagents | 第26-27页 |
| 2.1.5 Culture Medium | 第27页 |
| 2.1.6 Instruments | 第27页 |
| 2.2 Methods | 第27-36页 |
| 2.2.1 Genetic Screening of GsMlPS2 and GsSNAP33 Genes Based on Alkali Stress Responsive Gene Regulatory Networks | 第27-28页 |
| 2.2.2 Gene Cloning and Sequence Analysis of GsMIPS2 & GsSNAP33 | 第28-29页 |
| 2.2.3 GsMIPS2 and GsSNAP33 Gene Expression and Characteristics Analysis under Stress conditions | 第29-31页 |
| 2.2.4 Tissue Specific Expression Analysis of GsSNAP33 Gene | 第31页 |
| 2.2.5 Bioinformatics Analysis of GsMlPS2 & GsSNAP33 | 第31页 |
| 2.2.6 Transient Expression and Protein Subcellular localization of GsSNAP33 | 第31-33页 |
| 2.2.7 Functional Analysis of GsMIPS2 | 第33-34页 |
| 2.2.8 Expression Analysis of Abiotic Stress Related Marker Genes | 第34-36页 |
| 3 Results | 第36-63页 |
| 3.1 Functional Analysis of Wild Soybean Gene GsMJPS2 and its Physiological and Molecular Mechanisms under Salt Stress Conditions | 第36-49页 |
| 3.1.1 Determination of GsMIPS2 Gene Expression Profile under Salt Stress Conditions | 第36-37页 |
| 3.1.2 Sequence Analysis of GsMIPS2 Gene | 第37-39页 |
| 3.1.3 Identification of the GsMIPS2 Overexpression OX and atmips2 Plants | 第39-40页 |
| 3.1.4 Overexpression of GsMIPSl Conferred Increased Salt Tolerance at Various Growth Stages of Arabidopsis thaliana | 第40-47页 |
| 3.1.5 GsMIPS2 Overexpression Mediated Induced Expression of Stress Responsive Genes | 第47-49页 |
| 3.2 Functional Analysis of Wild Soybean Gene GsSNAP | 第49-52页 |
| 3.2.1 Cloning and Sequence Analysis of GsSNAP33 Gene | 第49-50页 |
| 3.2.2 Bioinformatics Analysis of GsSNAP33 Gene Family | 第50-52页 |
| 3.3 Determination of GsSNAP33 Gene Expression Profile under Salt, Alkali, ABA and PEG Stress Conditions | 第52-56页 |
| 3.4 Tissue Specific Expression Level Analysis of GsSNAP33 Gene in Glycine soja | 第56-57页 |
| 3.5 Generation of GsSNAP33 Overexpression lines | 第57-60页 |
| 3.5.1 Construction of Plant Expression Vector | 第57-58页 |
| 3.5.2 Transformation of pCAMBIA2300-GsSNAP33 Construct into Arabidopsis by Floral Dip Method | 第58-59页 |
| 3.5.3 Selection of GsSNAP33 Overexpressed Lines | 第59-60页 |
| 3.6 Subcellular Localization Analysis of GsSNAP33 Protein | 第60-63页 |
| 4 Discussion | 第63-68页 |
| 4.1 Diversity of GsMIPS2 and GsSNAP33 Genes in Plant Kingdom | 第63-64页 |
| 4.2 GsMIPS2 Gene Expression was Identified to be Up-regulated under Salt Stress Conditions | 第64页 |
| 4.3 GsMIPS2 Overexpression Confers Enhanced Salt Tolerance at Various Growth Stages of Arabidopsis thaliana | 第64-65页 |
| 4.4 GsMIPS2 Overexpression Alleviate Impacts of Stress Injuries at Mature Plant Stage | 第65页 |
| 4.5 GsMIPS2 Overexpression Results in Up-regulation of Various Abitic Stress-related Marker Genes | 第65-66页 |
| 4.6 GsSNAP33 Gene can be Induced under Salt, Alkali, PEG and ABA Stress Conditions | 第66-67页 |
| 4.7 GsSNAP33 is a Plasma Membrane Protein | 第67-68页 |
| 5 Conclusion | 第68-69页 |
| Aknowledgement | 第69-70页 |
| References | 第70-79页 |
| Papers published in the period of Ph. M. eduction | 第79页 |
