| ABSTRACT | 第1-8页 |
| TABLE OF CONTENTS | 第8-11页 |
| LIST OF FIGURES | 第11-13页 |
| LIST OF TABLES | 第13-14页 |
| CHAPTER | 第14-90页 |
| Ⅰ INTRODUCTION AND LITERATURE REVIEW | 第14-27页 |
| Rice as a model species for genome research | 第14-16页 |
| The large-insert DNA cloning systems and their utilities in genome research | 第16-20页 |
| Transforming plants with BIBACs or TACs carrying large DNA fragments | 第20-23页 |
| Genome-wide physical mapping | 第23-27页 |
| Ⅱ A PLANT-TRANSFORMATION-COMPETENT BIBAC-BASED INTEGRATED PHYSICAL MAP OF JAPONICA RICE FOR FUNCTIONAL ANALYSIS AND GENETIC ENGINEERING OF THE RICE GENOME | 第27-68页 |
| Abstract | 第27-28页 |
| Introduction | 第28-31页 |
| Materials and Methods | 第31-40页 |
| Source BIBAC and BAC libraries | 第31-35页 |
| Isolation and purification of BAC/BIBAC DNA | 第35-37页 |
| BAC and BIBAC fingerprinting and contig assembly | 第37-39页 |
| Library screening | 第39页 |
| BAC end sequencing | 第39页 |
| Availability of the integrated BAC/BIBAC-based physical map | 第39-40页 |
| Results and Discussion | 第40-68页 |
| BAC and BIBAC clones | 第40-42页 |
| BIBAC and BAC Fingerprinting | 第42-43页 |
| Contig Assembly and Manual Editing | 第43-51页 |
| Contig Reliability | 第51-57页 |
| BIBAC/BAC end sequencing and analysis | 第57-61页 |
| Utility of the BAC/BIBAC-based physical map for functional analysis of the rice genome sequence | 第61-62页 |
| Construction of a fine integrated genetic and physical map of rice chromosome 8 | 第62-68页 |
| Ⅲ CONSTRUCTION OF PLANT-TRANSFORMATION-COMPETENT BIBAC AND TAC LIBRARIES OF WILD AFRICA RICE (ORYZA BARTHII) FOR FUNCTIONAL ANALYSIS AND COMPARATIVE GENOMICS | 第68-90页 |
| Abstract | 第68-69页 |
| Introduction | 第69-71页 |
| Materials and Methods | 第71-78页 |
| Preparation of BIBAC and TAC vectors | 第71-73页 |
| Preparation of high-molecular-weight (HMW) DNA from nuclei | 第73-74页 |
| BIBAC and TAC Library construction | 第74-78页 |
| Analysis of BIBAC and TAC clones and library assembly | 第78页 |
| Results and Discussion | 第78-90页 |
| Construction of BIBAC library | 第78-79页 |
| Construction of TAC library | 第79-80页 |
| Analysis of BIBAC clones | 第80-84页 |
| Analysis of TAC clones | 第84-90页 |
| SUMMARY | 第90-92页 |
| REFERENCES | 第92-100页 |
| VITA | 第100-104页 |
| ACKNOWLEDGMENTS | 第104-105页 |
| APPENDIX (contigs of chromosome 8 of Oryza sativa L. ssp. japonica cv. Nipponbare) | 第105-115页 |
