| Abstract | 第6-7页 |
| Chapter1 | 第8-37页 |
| 1.Introduction | 第8-37页 |
| 1.1 Classification of Candidiasis (candidal infections) | 第10-11页 |
| 1.2 Background history of Candidiasis | 第11-12页 |
| 1.3 Virulence and pathogenicity of C.albicans. | 第12-23页 |
| 1.4 Diagnose and treatment of candidiasis | 第23-27页 |
| 1.5 Fungal vaccines | 第27-31页 |
| 1.6 DNA Vaccines | 第31-36页 |
| 1.7 Justification and objective | 第36-37页 |
| Chapter Ⅱ | 第37-48页 |
| 2 Materials and methods | 第37-48页 |
| 2.1 Preparation of DNA vaccine pD-HSP90C | 第37-41页 |
| 2.2 Immunization of mice and collection of serum | 第41-44页 |
| 2.3 ELISA to detect specific antibody titers in serum of mice immunized with DNAvaccine pD-HSP90C | 第44-45页 |
| 2.4 ELISPOT to detect IL-2 in serum of mice immunized with DNA vaccinepD-HSP90C | 第45-46页 |
| 2.5 Assessment of DNA vaccine protection against systemic C. albicans infection | 第46-48页 |
| Chapter Ⅲ | 第48-54页 |
| 3. Results | 第48-54页 |
| 3.1 Humoral response mediated by DNA vaccine pD-HSP90C | 第48-51页 |
| 3.2 Detection of IL-2、IL-4 in serum of mice immunized with DNA vaccinepD-HSP90C | 第51-52页 |
| 3.3 Protection against systemic C. albicans infection mediated by DNA vaccine | 第52-54页 |
| Chapter Ⅳ | 第54-57页 |
| 4 Discussion | 第54-57页 |
| Chapter Ⅴ | 第57-58页 |
| 5 Conclusions | 第57页 |
| 5 Conclusions | 第57-58页 |
| References | 第58-64页 |
| Dedication | 第64-65页 |
| ACKNOWLEDGEMENTS | 第65页 |
