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Multi-Environment QTL Analysis of Grain Morphology Traits and Fine Mapping of a Kernel-Width QTL in Zheng58 × SK Maize Population

摘要第7-8页
Abstract第8页
CHAPTER I INTRODUCTION第13-26页
    1.1 Breeding for Maize Improvement第13-15页
        1.1.1 Maize (Zea mays L.)第13-14页
        1.1.2 Genetics of kernel development第14-15页
        1.1.3 Maize kernel related traits and yield第15页
    1.2 Positional Cloning Analysis to Dissect Complex Trait第15-20页
        1.2.1 Mapping population第16页
        1.2.2 Choice of mapping population第16-17页
        1.2.3 Construction of linkage map第17-18页
        1.2.4 QTL analysis and mapping第18-19页
        1.2.5 Fine mapping第19页
        1.2.6 Positional cloning第19-20页
    1.3 QTL Analysis of Maize Grain Morphology Traits Based on Recombinant Inbred Line (RIL) Population第20-22页
        1.3.1 QTLs for maize kernel traits第20-22页
        1.3.2 QTL analysis in RIL population第22页
    1.4. Fine Mapping and Map-based Cloning Using Heterogeneous Inbred Family第22-24页
        1.4.1 Fine mapping of grain width QTL第23-24页
        1.4.2 QTL fine mapping using HIF第24页
    1.5 Objectives and Significance第24-26页
CHAPTER II MATERIALS AND METHODS第26-32页
    2.1 QTL Analysis of Maize Grain Morphology Traits第26-29页
        2.1.1 Plant material and field experiments第26页
        2.1.2 Phenotypic measurements of kernel traits第26-27页
        2.1.3 Linkage map construction and QTL mapping第27-29页
        2.1.4 Heritability estimation第29页
    2.2. Fine Mapping of Kernel Width QTL qKW-9.2第29-32页
        2.2.1 Selection and validation of HIF population第29-30页
        2.2.2 DNA extraction and PCR protocol第30页
        2.2.3 SSR markers analysis第30页
        2.2.4 Fine mapping of qKW-9.2 and gene annotation第30-32页
CHAPTER III RESULTS第32-51页
    3.1 QTL Analysis of Maize Grain Morphology Traits第32-46页
        3.1.1 Phenotypic variation in kernel related traits第32-34页
        3.1.2 Heritability and correlation第34-35页
        3.1.3 QTL mapping for kernel traits in RIL populations第35-42页
            a) Kernel length (KL)第35-36页
            b) Kernel width (KW)第36-41页
            c) Kernel thickness (KT)第41-42页
            d) 100-kernel weight (HKW)第42页
        3.1.4 QTL detection using BLUP data第42-46页
    3.2. Fine Mapping of Kernel Width QTL qKW-9.2第46-51页
        3.2.1 Validation of the identified QTL qKW-9.2第46-48页
        3.2.2 Fine mapping of qKW-9.2第48页
        3.2.3 Predicted genes at the locus qKW-9.2第48-51页
CHAPTER IV DISCUSSION第51-58页
    4.1 QTL Analysis of Maize Grain Morphology Traits第51-54页
        4.1.1 QTL analysis in RIL population第51-52页
        4.1.2 QTL cluster analysis using BLUP data第52-53页
        4.1.3 Stability of the identified QTL第53页
        4.1.4 Morpho-physiological possibilities for traits impacts of the identified QTLs第53-54页
    4.2 Fine mapping of qKW-9.2第54-58页
        4.2.1 Fine mapping of q KW-9 using HIF第54-55页
        4.2.2 Putative candidate genes第55-58页
CHAPTER V CONLUSION第58-59页
CHAPTER VI: FURTHER WORK AND PROSPECTIVE第59-60页
REFERENCES第60-71页
APPENDICES第71-80页
Acknowledgement第80-81页
Curriculum Vitae第81-83页
List of publications during Ph D tenure第83-84页

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