| Abstract | 第8-9页 |
| 摘要 | 第10-12页 |
| Chapter 1 General Introduction | 第12-31页 |
| 1.1 Overview of Marine Biofouling | 第12-14页 |
| 1.1.1 Marine Biofouling and Impacts | 第12-13页 |
| 1.1.2 General Process of Fouling Formation | 第13-14页 |
| 1.2 Macroalgae as Biofouling Organism | 第14-18页 |
| 1.2.1 Algae as Biofouling | 第14-16页 |
| 1.2.2 Green Tide | 第16-17页 |
| 1.2.3 Aggregation of Algae | 第17-18页 |
| 1.3 Biology of Ulva | 第18-24页 |
| 1.3.1 Taxonomy | 第18-19页 |
| 1.3.2 Life Cycle and Reproduction of Ulva | 第19-21页 |
| 1.3.3 Spore of Ulva | 第21-24页 |
| 1.4 Settlement Cues of Ulva Spores | 第24-29页 |
| 1.4.1 Physical Cues | 第24-26页 |
| 1.4.2 Biological Cues | 第26-28页 |
| 1.4.3 Chemical Cues | 第28-29页 |
| 1.5 Research Aim, Scope and Significance | 第29-31页 |
| 1.5.1 Research Scope | 第30页 |
| 1.5.2 Research Significance | 第30-31页 |
| Chapter 2 Effects of Compounds Isolated From Ulva intestinalis onSettlement of Its Spores | 第31-53页 |
| 2.1 Introduction | 第31-32页 |
| 2.2 Methodologies | 第32-37页 |
| 2.2.1 Biomaterials | 第32页 |
| 2.2.2 Chemical Reagents | 第32页 |
| 2.2.3 Equipment | 第32-33页 |
| 2.2.4 Methods | 第33-37页 |
| 2.2.5 Data Analysis | 第37页 |
| 2.3 Results | 第37-48页 |
| 2.3.1 Effect of Crude Extracts on Settlement of U. intestinalis Spores | 第37-38页 |
| 2.3.2 Inducing Active Compound from the Hexane Extract | 第38-42页 |
| 2.3.3 Inducing Active Compound from the Dichloromethane Extract | 第42-48页 |
| 2.4 Discussion | 第48-53页 |
| 2.4.1 Effect of Fatty Acid C 16:4 on Settlement of U. intestinalis Spores | 第49-51页 |
| 2.4.2 Effect Dibutyl Phthalate on Settlement of U. intestinalis Spores | 第51-52页 |
| 2.4.3 Fatty Acid C16:4 and Dibutyl Phthalate as Settlement Inducer | 第52-53页 |
| Chapter 3 Composition of Fatty Acid Related Compounds in ActiveFractions from Ulva intestinalis and Their Activity | 第53-67页 |
| 3.1 Introduction | 第53-54页 |
| 3.2 Methodologies | 第54-57页 |
| 3.2.1 Material | 第54页 |
| 3.2.2 Chemical Reagents | 第54-55页 |
| 3.2.3 Equipment | 第55页 |
| 3.2.4 Methods | 第55-57页 |
| 3.3 Data Analysis | 第57-58页 |
| 3.4 Results | 第58-62页 |
| 3.4.1 Compounds in Active Fractions HU, FI and FII | 第58-59页 |
| 3.4.2 Fatty Acids in Active Fractions HU, FI and FII | 第59-60页 |
| 3.4.3 Simple, Complex and Sterol Lipid in Active Fractions HU, FI and FII | 第60-61页 |
| 3.4.4 Effect of Seven Fatty Acids on Settlement of U. intestinalis Spores | 第61-62页 |
| 3.5 Discussion | 第62-67页 |
| 3.5.1 Compounds in Active Fractions HU,FI and FII | 第62-63页 |
| 3.5.2 Fatty Acids, Simple Lipids, Complex Lipids and Sterols in Active FractionsHU, FI and FII | 第63-65页 |
| 3.5.3 Effect of Seven Fatty Acids on Settlement of U. intestinalis Spores | 第65-67页 |
| Chapter 4 Effect of Bacteria Biofilms on Settlement of Ulva intestinalisSpores | 第67-83页 |
| 4.1 Introduction | 第67-68页 |
| 4.2 Methodologies | 第68-73页 |
| 4.2.1 Chemical Reagent | 第68-69页 |
| 4.2.2 Equipment | 第69页 |
| 4.2.3 Cultural Medium of Bacteria | 第69页 |
| 4.2.4 Samples Collection | 第69-70页 |
| 4.2.5 Extraction of Total Genomic DNA and Hightrougput Sequencing of 16SrRNA | 第70页 |
| 4.2.6 Analysis of Bacteria Community Structure Associated with U. intestinalis | 第70-71页 |
| 4.2.7 Isolation and Identification of Bacteria Associated with U. intestinalis | 第71-72页 |
| 4.2.8 Biofilm Preparation | 第72页 |
| 4.2.9 Bioassay on Settlement of U.intestinalis Spores | 第72页 |
| 4.2.10 Observation of Aggregation of U.intestinalis Spores on Biofilmed Surface | 第72-73页 |
| 4.2.11 Data Analysis | 第73页 |
| 4.3 Results | 第73-78页 |
| 4.3.1 Bacterial Community Associated with U. intestinalis | 第73-76页 |
| 4.3.2 Isolation and Identification of Bacteria Associated with U. intestinalis | 第76-78页 |
| 4.3.3 Effect of Bacterial Biofilms on Settlement of U. intestinalis Spores | 第78页 |
| 4.3.4 Aggregation of Uintestinalis Spores on Biofilmed Surfaces | 第78页 |
| 4.4 Discussion | 第78-83页 |
| 4.4.1 Bacterial Community Structure Associated with U. intestinalis | 第78-80页 |
| 4.4.2 Effect of Bacterial Biofilms on Settlement of U. intestinalis Spores | 第80-81页 |
| 4.4.3 Aggregation of U. intestinalis Spores on Biofilmed Surfaces | 第81-83页 |
| Chapter 5 Summary and Future Research | 第83-85页 |
| 5.1 Summary | 第83-84页 |
| 5.2 Future Research | 第84-85页 |
| References | 第85-102页 |
| Appendix 1 Toxic effect of subfractions of HU on I. intesdnalis spores | 第102-103页 |
| Appendix 2 NMR and HI ESI MS spectra of active compound HU6 | 第103-104页 |
| Appendix 3. Toxic effect of subfrations from DMU | 第104-105页 |
| Appendix 4 Toxic effect of subfractions from FI | 第105-106页 |
| Appendix 5 NMR spectra of fraction TFI2 | 第106-107页 |
| Appendix 6 Toxic effect of subfractions from FII | 第107-108页 |
| Appendix 7 HR ESI MS spectrum of fraction FII1 | 第108-109页 |
| Appendix 8 The compounds in active fractions HU, FI and FII | 第109-111页 |
| Appendix 9 Simple, complex lipids, and sterols in active fractions HU,FI and FII | 第111-117页 |
| Appendix 10 Toxicity effect of fatty acids on U. intestinalis spores | 第117-118页 |
| Achievements | 第118-119页 |
| Acknowledgements | 第119页 |
