| 摘要 | 第8-9页 |
| ABSTRACT | 第9页 |
| List of Abbreviations | 第10-13页 |
| CHAPTER 1 INTRODUCTION | 第13-16页 |
| 1.1 BACKGROUND AND MOTIVATION | 第13-16页 |
| CHAPTER 2 LITERATURE REVIEW | 第16-47页 |
| 2.1 BREAST CANCER FACTS: | 第16-17页 |
| 2.2 BREAST CANCER OCCURRENCE | 第17-18页 |
| 2.3 SYMPTOMS | 第18页 |
| 2.4 SCREENING: | 第18-21页 |
| 2.5 SERUM TUMOR MARKERS AND APTAMER SENSING DETECTION | 第21-22页 |
| 2.6 SELEX TECHNOLOGY TO GENERATE NUCLEIC ACID APTAMERS | 第22页 |
| 2.7 HISTORY AND THEORETICAL BACKGROUND | 第22-35页 |
| 2.7.1 In Vitro Selection of Functional Oligonucleotides and the Origins of Biochemical Activity | 第22-23页 |
| 2.7.2 A Brief History of In Vitro Selection | 第23-25页 |
| 2.7.3 Lessons from the Aptamers, Ribozymes, Deoxy ribozymes Generated by In Vitro Selection | 第25-32页 |
| 2.7.4 Synthetic Approaches to Understanding the Natural Origins of Function | 第32-35页 |
| 2.8 SOME NON-CLASSICAL APPROACHES OF APTAMERS SELECTION | 第35-36页 |
| 2.9 THE RECENT PROGRESS OF SOME MODERN-APPROACHES OF APTAMERS SELECTION | 第36-43页 |
| 2.9.1 Libraries Optimization for Aptamer Selection | 第37-38页 |
| 2.9.2 Primer sites reduction | 第38-39页 |
| 2.9.3 The methods of in-vitro selection | 第39-41页 |
| 2.9.4 SELEX-on-a-chip | 第41-42页 |
| 2.9.5 Other Optional separation methods | 第42-43页 |
| 2.10 A NEW ASSAY FOR APTAMER DETERMINATION BY USING NGS | 第43-45页 |
| 2.10.1 Determination of high quality of resolution | 第43页 |
| 2.10.2 Determination in Precocious selection round | 第43-44页 |
| 2.10.3 Aptamer and Sequence Clustering Assays | 第44-45页 |
| 2.11 APTAMERS: CURRENT CHALLENGES AND FUTURE PROSPECTS | 第45-47页 |
| CHAPTER 3 MATERIALS AND METHODS | 第47-53页 |
| 3.1. MATERIALS | 第47页 |
| 3.2 METHODS | 第47-53页 |
| 3.2.1 Serum collection and processing | 第47页 |
| 3.2.2 Brest cancer serum-magnetic beads combination and combined serum-magnetic beads treatment | 第47-48页 |
| 3.2.3 Brest cancer serum proteins screening aptamers | 第48-49页 |
| 3.2.4 Ds DNA library Preparation | 第49-50页 |
| 3.2.5 Ss DNA library Preparation | 第50-51页 |
| 3.2.5.1 Quantitive real time PCR for ssDNA production: | 第50页 |
| 3.2.5.2 Polyacrylamide Gel electrophoresis for ssDNA library Preparation and ss DNA purification | 第50-51页 |
| 3.2.6 SsDNA library concentration detection | 第51页 |
| 3.2.7 Specificity detection for the ss DNA combined to target protein | 第51-53页 |
| 3.2.7.1 Gel electrophoresis (PAGE) test | 第51-52页 |
| 3.2.7.2 Real time quantitative PCR method | 第52-53页 |
| CHAPTER 4 RESULTS, DISCUSSION AND CONCLUSION | 第53-61页 |
| 4.1 RESULTS | 第53-59页 |
| 4.1.1 Brest cancer serum proteins-aptamer screening results | 第53-57页 |
| 4.1.1.1 Quantitive Real time PCR Result for First and second Rounds of Screening | 第53-54页 |
| 4.1.1.2 Quantitive Real time PCR Result for Fourth Round of Screening | 第54-55页 |
| 4.1.1.3 Quantitive Real time PCR Result for 5th Round of Screening | 第55-56页 |
| 4.1.1.4 Quantitive Real time PCR Result for 9th Round of Screening | 第56-57页 |
| 4.1.2 SsDNA secondary library preparation | 第57-58页 |
| 4.1.3 Sequencing | 第58-59页 |
| 4.2 DISCUSSION | 第59-60页 |
| 4.3 CONCLUSION AND FUTURE WORK | 第60-61页 |
| REFERENCES | 第61-71页 |
| ACKNOWLEDGEMENTS | 第71-72页 |
| LIST OF PUBLICATIONS | 第72页 |
