| Abstract | 第6-7页 |
| Chapter 1 Literature review | 第12-25页 |
| 1.1 Introduction | 第12-14页 |
| 1.2 Maturation of pig oocytes in vitro | 第14-22页 |
| 1.2.1 Oocyte meiotic resumption | 第14-15页 |
| 1.2.2 Cytoplasmic maturation | 第15-16页 |
| 1.2.3 MPF and MAPK | 第16-17页 |
| 1.2.3.1 MPF | 第16-17页 |
| 1.2.3.2 MAPK | 第17页 |
| 1.2.4 Factors affecting the development and quality of pig oocytes matured in vitro | 第17-22页 |
| 1.2.4.1 Donor age & Follicular Factors | 第17-20页 |
| 1.2.4.2 Anti-oxygen | 第20页 |
| 1.2.4.3 Growth facotrs | 第20页 |
| 1.2.4.4 Hormone | 第20-21页 |
| 1.2.4.5 Other | 第21-22页 |
| 1.3 cAMP and PKA pathway | 第22-24页 |
| 1.4 Aim of this study | 第24-25页 |
| Chapter 2 Methods and materials | 第25-34页 |
| 2.1 Chemicals and media | 第25-26页 |
| 2.1.1 Basic meida | 第25-26页 |
| 2.1.2 IVM media | 第26页 |
| 2.1.3 SCNT media | 第26页 |
| 2.2 Main equipment | 第26-27页 |
| 2.3 Methods | 第27-32页 |
| 2.3.1 Collection and transportation of ovaries | 第27-28页 |
| 2.3.2 Oocytes collection and IVM | 第28页 |
| 2.3.3 Cumulus cell removal and polar body searching | 第28页 |
| 2.3.4 Establishment of porcine fibroblast cell lines | 第28-29页 |
| 2.3.5 Preparation of donor cells | 第29页 |
| 2.3.6 Nuclear transfer | 第29-30页 |
| 2.3.7 In vitro culture of embryos | 第30页 |
| 2.3.8 Staining | 第30-32页 |
| 2.4 Experiments Design | 第32-33页 |
| 2.5 Statistical analysis | 第33-34页 |
| Chapter 3 Results and analysis | 第34-40页 |
| 3.1 Effects of duration of maturation,hormone free and chemical treatment on development of pig embryo cultured in vitro | 第34-36页 |
| 3.1.1 Effect of difference in vitro maturation durations on development of porcine parthenogenetic activation embryo | 第34-35页 |
| 3.1.2 Effects of the duration of exposure to hormone supplements on development of pig oocytes cultured in vitro | 第35页 |
| 3.1.3 Effect of CB and 6-DMAP treatment on development of pig parthenogenetic embryos cultured in vitro | 第35-36页 |
| 3.2 Effect of fusion voltage on rate of fusion and lysis of oo-plasm | 第36页 |
| 3.3 Effect of dbcAMP on oocytes were at GV stage | 第36-40页 |
| 3.3.1 Ability of dbcAMP inhibit GVBD of pig oocytes matured in vitro | 第36-38页 |
| 3.3.2 Effect of different dbcAMP treatment durations on maturation of pig oocytes matured in vitro | 第38-39页 |
| 3.3.3 Effect of different dbcAMP treatment durations on development of SCNT and PA embryo cultured in vitro | 第39-40页 |
| Chapter 4 Discussion | 第40-45页 |
| 4.1 Effects of duration of maturation,hormone free and chemical treatment on development of pig embryo cultured in vitro | 第40-42页 |
| 4.1.1 Effect of different maturation durations on maturation of pig oocytes cultured in vitro | 第40-41页 |
| 4.1.2 Effect of the duration of exposure to hormone supplements on development of pig oocytes cultured in vitro | 第41页 |
| 4.1.3 Effect of CB and 6-DMAP treatment on development of pig parthenogenetic embryo cultured in vitro | 第41-42页 |
| 4.2 Effect of fusion voltage on rate of fusion and lysis of oo-plasm | 第42页 |
| 4.3 Effect of dbcAMP on pig oocytes and embryo cultured in vitro | 第42-45页 |
| 4.3.1 Ability of dbcAMP inhibited GVBD of pig oocytes matured in vitro | 第42-43页 |
| 4.3.2 Effect of different dbcAMP treatment durations on maturation of pig oocyte matured in vitro | 第43页 |
| 4.3.3 Effect of different dbcAMP treatment durations on development of SCNT and PA embryo cultured in vitro | 第43-45页 |
| Chapter 5 Conclusion | 第45-47页 |
| Reference | 第47-53页 |
| Appendix Ⅰ Components of basic medie | 第53-54页 |
| Acknowledgement | 第54页 |
