| Abstract | 第6页 |
| Abbreviations | 第7-8页 |
| 1 Review of literature | 第8-22页 |
| 1.1 Overview | 第8-9页 |
| 1.2 What is cuticle and cuticular wax | 第9-10页 |
| 1.3 Cuticular wax biosynthesis | 第10-11页 |
| 1.4 Multiple elongation system | 第11-13页 |
| 1.5 Synthesis of very long chain fatty acid wax precursors | 第13-14页 |
| 1.6 Regulation of wax biosynthesis | 第14-16页 |
| 1.7 Glossy mutant (gl) defective or altered in wax production | 第16-18页 |
| 1.8 Agrobacterium-mediated transformation | 第18-19页 |
| 1.9 Selectable and reporter marker genes | 第19-20页 |
| 1.10 T-DNA intertional mutagenesis | 第20-22页 |
| 2 Aim and significance | 第22-23页 |
| 3 Materials and methods | 第23-32页 |
| 3.1 Identification and sequence analysis of GL1-homologous genes in rice | 第23-24页 |
| 3.2 Plant growth and treatments | 第24-25页 |
| 3.3 Transcript level analysis of GL1-like gene family in rice | 第25-26页 |
| 3.4 Over-expression of homologous genes | 第26-28页 |
| 3.5 Genotypic screening of mutant osgl1-2 plants | 第28页 |
| 3.6 Phenotyping and measurements of transgenic and mutant plants | 第28-30页 |
| 3.6.1 Stress in field condition | 第28-29页 |
| 3.6.2 Measurement of plant height, tiller number and observation of plants development | 第29页 |
| 3.6.3 Chlorophyll leaching and water loss measurement | 第29-30页 |
| 3.7 Scanning electronic microscopy (SEM) and transmission electron microscopy (TEM) observations | 第30-31页 |
| 3.8 Wax extraction and quantification | 第31-32页 |
| 4 Results | 第32-51页 |
| 4.1 Identification of GL1 homologous genes in rice | 第32-36页 |
| 4.2 Phylogenetic analysis of GL1-like gene family | 第36-38页 |
| 4.3 Expression profile of OsGL1-like gene family in tissues and organs | 第38-41页 |
| 4.4 Responsiveness of OsGL1 genes to abiotic stresses | 第41-42页 |
| 4.5 Over-expression of the homologous genes | 第42-43页 |
| 4.6 Reduced growth, altered drought resistance and cuticular permeability in OsGL1-2 over-expression and mutant rice plants | 第43-48页 |
| 4.7 Altered wax accumulation in the OsGL1-2 over-expression and mutant rice plants | 第48-51页 |
| 5 Discussions | 第51-57页 |
| 5.1 OsGL1 homologs, WAX2, and CER1 are from same ancestor | 第51-52页 |
| 5.2 Expressions of the OsGL1-like genes are tissue-or organ specific and osgl1-2 mutant is sensitive to drought | 第52-54页 |
| 5.3 The osgl1-2 mutant affects cuticular wax accumulation and biosynthesis | 第54-56页 |
| 5.4 Future prospectus | 第56-57页 |
| References | 第57-67页 |
| Protocols | 第67-82页 |
| Resume | 第82-84页 |
| Acknowledgement | 第84-86页 |
