| 摘要 | 第1-8页 |
| Abstract | 第8-10页 |
| Part Ⅰ Introduction | 第10-24页 |
| 1. Introduction of nanomaterials | 第10页 |
| 2. Introduction of magnetic nanomaterials | 第10-12页 |
| 3. Progress in the synthesis method of magnetic nanoparticles | 第12-14页 |
| ·Chemical coprecipitation | 第12页 |
| ·Thermal decomposition | 第12页 |
| ·Microemulsion | 第12-13页 |
| ·Hydrothermal synthesis | 第13页 |
| ·Sonochemical synthesis | 第13-14页 |
| 4. Surface functionalization of magnetic nanoparticles | 第14-16页 |
| ·Small molecules and surfactants coating | 第14-15页 |
| ·Polymers coating | 第15页 |
| ·Biological molecules coating | 第15页 |
| ·Silica coating | 第15-16页 |
| 5. Application of magnetic nanoparticles | 第16-19页 |
| ·Applications in catalysis | 第16页 |
| ·Applications in drug | 第16-17页 |
| ·Applications in bio-separation | 第17页 |
| ·Applications in environmental detection | 第17-19页 |
| References | 第19-24页 |
| Part Ⅱ Main research work | 第24-78页 |
| Section 1. Mixed hemimicelles solid-phase extraction of chlorophenols in environmentalwater samples with 1-hexadecyl-3-methylimidazolium bromide-coated Fe_3O_4 magneticnanoparticles with high-performance liquid chromato graphic analysis | 第24-44页 |
| ·Introduction | 第24-26页 |
| ·Experimental | 第26-28页 |
| ·Chemicals and materials | 第26页 |
| ·Preparation of magnetic Fe_3O_4 nanoparticles | 第26-27页 |
| ·Characterization | 第27页 |
| ·SPE procedures | 第27-28页 |
| ·HPLC-UV analysis of environmental water samples | 第28页 |
| ·Results and discussion | 第28-38页 |
| ·Physical characteristics of Fe_3O_4 nanoparticles | 第28-31页 |
| ·Isoelectric point | 第31-32页 |
| ·Optimization of the analysis conditions | 第32-38页 |
| ·Effect of the amount of C_(16)mimBr on the adsorption of CPs | 第32页 |
| ·Effect of the amount of Fe_3O_4 NPs | 第32-33页 |
| ·Effect of solution pH | 第33-34页 |
| ·Effect of ionic strength | 第34-35页 |
| ·Standing and magnetic separating time | 第35页 |
| ·Effect of solution volume and desorption conditions | 第35-36页 |
| ·Analytical performance and application | 第36-38页 |
| ·Conclusions | 第38-39页 |
| References | 第39-44页 |
| Section 2. Synthesis of a magnetic molecularly imprinted polymer for the selectiveextraction of phenolic compounds from environmental water samples withhigh-performance liquid chromatographic analysis | 第44-63页 |
| ·Introduction | 第44-45页 |
| ·Experimental | 第45-49页 |
| ·Chemicals and materials | 第45-46页 |
| ·Procedures for the preparation of magnetic 4-NP-imprinted polymeric microspheres | 第46-47页 |
| ·Characterization | 第47-48页 |
| ·Adsorption experiments | 第48页 |
| ·Application of magnetic molecularly imprinted polymer for extraction of phenolic compounds from environmental water samples | 第48页 |
| ·Chromatographic system and conditions | 第48-49页 |
| ·Results and discussion | 第49-59页 |
| ·Characterizations of the magenitc molecularly imprinted polymers | 第49-52页 |
| ·Adsorption study | 第52-54页 |
| ·Effect of extraction and desorption conditions | 第54-58页 |
| ·Analytical performance and the application of the magenitc molecularly imprinted polymers to real samples | 第58-59页 |
| ·Conclusions | 第59-60页 |
| References | 第60-63页 |
| Section 3. Adenosine Triphosphate assay employing a label-free aptamer andfunctional magnetic nanoparticles | 第63-78页 |
| ·Introduction | 第63-64页 |
| ·Experimental | 第64-66页 |
| ·Chemicals and apparatus | 第64页 |
| ·Preparation of the oligonucleotide functioned magnetic nanoparticles | 第64-65页 |
| ·Fabrication of complementary aptamer duplexes-modified magnetic nanoparticles | 第65页 |
| ·Incubation of ATP with the aptamer duplexes-modified magnetic nanoparticles | 第65-66页 |
| ·ATP assay and the selectivity study | 第66页 |
| ·Results and discussion | 第66-74页 |
| ·ATP assay strategy | 第66-67页 |
| ·Optimization of conditions for target detection | 第67-71页 |
| ·Optimization of the correlation parameters for the complementary aptamer duplexes-modified magnetic nanoparticles | 第67-69页 |
| ·Effect of the EB concentration | 第69-70页 |
| ·Optimization of the target ATP incubation temperature and time | 第70-71页 |
| ·Analytical performance and application | 第71-74页 |
| ·Conclusion | 第74-75页 |
| References | 第75-78页 |
| Conclusions | 第78-80页 |
| 致谢 | 第80-82页 |
| Appendix: Paper published during the graduate | 第82页 |
