| Abstract in Chinese | 第1-3页 |
| Abstract in English | 第3-5页 |
| Abbreviations | 第5-10页 |
| Chapter 1 General Introduction | 第10-29页 |
| ·The current situation of crude drug | 第10-11页 |
| ·About the physicochemical constant of crude drug | 第11-15页 |
| ·The hydrolysis rate constant | 第12页 |
| ·The binding constant | 第12-13页 |
| ·The ionization constants | 第13-14页 |
| ·Traditional detection methods | 第14-15页 |
| ·A brief introduction to CE | 第15-28页 |
| ·Historical background of CE | 第16-17页 |
| ·The theory of electrophoresis | 第17-19页 |
| ·Electro-osmotic flow (EOF) | 第17-18页 |
| ·Mobility and migration time | 第18页 |
| ·Dispersion | 第18-19页 |
| ·Resolution | 第19页 |
| ·Modes of operation | 第19-20页 |
| ·Electrochemical Detection (ED) | 第20-27页 |
| ·About CE-ED | 第20-25页 |
| ·Conductometric detection | 第21-22页 |
| ·Potentiometric detection | 第22页 |
| ·Amperometric detection | 第22-25页 |
| ·The application of CE-ED in the investigation of crude drug | 第25-27页 |
| ·Application of CE in the determination of physicochemical constant | 第27-28页 |
| ·The researches in this thesis | 第28-29页 |
| Chapter 2 Determination of the hydrolysis rate constants and activation energyof aesculin with CE-ED | 第29-43页 |
| ·Introduction | 第29-31页 |
| ·Experimental | 第31-33页 |
| ·Chemicals | 第31-32页 |
| ·Apparatus | 第32-33页 |
| ·Hydrolysis procedures | 第33页 |
| ·Results and discussion | 第33-42页 |
| ·Kinetic equations | 第33-34页 |
| ·The optimum conditions for electrophoresis | 第34-36页 |
| ·Amperometric Detection | 第36-38页 |
| ·Detection limit, linearity and repeatability | 第38-39页 |
| ·Determination of the hydrolysis rate constants of aesculin | 第39-40页 |
| ·Determination of the activation energy of aesculin | 第40-42页 |
| ·Conclusions | 第42-43页 |
| Chapter 3 Kinetic study on hydrolysis of chlorogenic acid in aqueous solution by CE-ED | 第43-59页 |
| ·Introduction | 第43-46页 |
| ·Theoretical | 第46页 |
| ·Experimental | 第46-48页 |
| ·Materials | 第46-47页 |
| ·Apparatus | 第47-48页 |
| ·Hydrolysis procedures | 第48页 |
| ·Results and discussion | 第48-58页 |
| ·Electrochemical Detection | 第48-50页 |
| ·The optimum conditions for electrophoresis | 第50-54页 |
| ·Effect of the buffer solution | 第50-52页 |
| ·Effect of the separation voltage | 第52页 |
| ·Effect of the injection time | 第52-54页 |
| ·Repeatability, linearity, detection limits | 第54-55页 |
| ·Kinetic study on hydrolysis of chlorogenic acid | 第55-58页 |
| ·Conclusion | 第58-59页 |
| Chapter 4 Study on the interactions between five crude medicine molecules of coumarin derivatives and bovine serum albumin by CE-AD | 第59-75页 |
| ·Introduction | 第59-62页 |
| ·Experimental | 第62-64页 |
| ·Capillary electrophoresis system | 第62-63页 |
| ·Chemical and preparation of solutions | 第63-64页 |
| ·Procedure | 第64页 |
| ·Results and discussion | 第64-74页 |
| ·The simple model of the interaction of coumarin derivative and BSA | 第64-65页 |
| ·Electrochemical Detection | 第65-67页 |
| ·The optimum conditions for electrophoresis | 第67-70页 |
| ·Effect of the injection time | 第67-68页 |
| ·Effect of the separation voltage | 第68-69页 |
| ·Effect of the pH of buffer | 第69-70页 |
| ·Effect of the concentration of buffer | 第70页 |
| ·Quantification and recovery of coumarin derivatives | 第70-72页 |
| ·The study on the interactions between coumarin derivatives and BSA | 第72-74页 |
| ·Conclusion | 第74-75页 |
| Chapter 5 Study on Ionization Constants of Coumarin derivatives by Linear Model with CE-AD | 第75-86页 |
| ·Introduction | 第75-76页 |
| ·Theoretical | 第76-79页 |
| ·The theory of the determination of ionization constant by CE | 第76-78页 |
| ·The theory of the determination of ionization constant by UV spectrophotometer | 第78-79页 |
| ·Experimental | 第79-82页 |
| ·Apparrtus | 第79-80页 |
| ·Materials | 第80-81页 |
| ·The procedures of the determination of ionization constant | 第81-82页 |
| ·The CE method | 第81页 |
| ·The UV spectrophotometer method | 第81-82页 |
| ·Results and discussion | 第82-85页 |
| ·The optimum conditions for electrophoresis | 第82-83页 |
| ·The determination of ionization constants of coumarin derivatives | 第83-85页 |
| ·The CE method | 第83-84页 |
| ·The UV spectrophotometer method | 第84-85页 |
| ·Conclusion | 第85-86页 |
| Reference | 第86-98页 |
| Acknowledgements | 第98-99页 |
| Publications during the author's M.S. study | 第99-100页 |
