| ABSTRACT | 第1页 |
| 摘要 | 第8-18页 |
| CHAPTER ONE:INTRODUCTION AND LITERATURE REVIEW | 第18-42页 |
| ·INTRODUCTION | 第18-21页 |
| ·LITERATURE REVIEW | 第21-42页 |
| ·Overview of tuberculosis worldwide | 第21-22页 |
| ·Treatment of tuberculosis | 第22页 |
| ·First line drugs | 第22页 |
| ·Second line drugs | 第22页 |
| ·Evolution of drug resistance in Mycobacterium tuberculosis | 第22-24页 |
| ·Clinical circumstance for resistance development | 第24-25页 |
| ·Compartmentalization of tuberculosis infection | 第25-26页 |
| ·Occurrence of mutation in mycobacterium | 第26-28页 |
| ·Mutation rate | 第27页 |
| ·Mutant antagonism | 第27-28页 |
| ·Adaptation of mycobacterium | 第28-29页 |
| ·Human studies of resistance emergence | 第29-30页 |
| ·Multi-drug resistant tuberculosis and global response on TB surveillance | 第30-33页 |
| ·Molecular mechanism of EMB action | 第33-34页 |
| ·Molecular mechanisms for the emergence of drug resistance | 第34-36页 |
| ·Challenge of embB306 as a marker | 第36-37页 |
| ·Molecular methods to detect drug resistance | 第37-41页 |
| ·Current methods used worldwide for ethambutol drug-resistance detection | 第37-41页 |
| ·Main Objectives of the study | 第41-42页 |
| CHAPTER TWO:MOLECULAR BEACON PROBE MELTING CURVE ANALYSIS FOR RAPID SCREENING OF ETHAMBUTOL DUG RESISTANCE IN MYCOBACTERIUM TUBERCULOSIS ISOLATES | 第42-64页 |
| ·Background | 第42页 |
| ·Material and method | 第42-47页 |
| ·Principle of methodology | 第42页 |
| ·Principle of the original molecular beacon probes | 第42-43页 |
| ·Principle of our modified molecular beacon probes melting curve analysis | 第43-44页 |
| ·Sample preparation | 第44-45页 |
| ·Boiling method | 第44页 |
| ·Bio-v DNA extraction Kit:protocol | 第44-45页 |
| ·Equipments used during the extraction | 第45页 |
| ·Primers and probes | 第45-46页 |
| ·Real-time PCR assay | 第46页 |
| ·Sensitivity | 第46页 |
| ·Specificity | 第46-47页 |
| ·Sequencing | 第47页 |
| ·Validation of the system | 第47页 |
| ·RESULTS | 第47-60页 |
| ·Primers and probe choice | 第47-48页 |
| ·Allele discrimination | 第48-50页 |
| ·Primer ratio optimization | 第50-52页 |
| ·Analytical Sensitivity test | 第52页 |
| ·Specificity test | 第52-53页 |
| ·Clinical samples tests | 第53-55页 |
| ·-Sequencing results | 第55-59页 |
| ·Drug information | 第59页 |
| ·Validation | 第59-60页 |
| ·Discussion | 第60-63页 |
| ·Conclusion | 第63-64页 |
| References | 第64-69页 |
| Acknowledgement | 第69页 |
