| 摘要 | 第5-6页 |
| Abstract | 第6-7页 |
| Chapter 1 Review ofbrachyurization and the study of RNA sequencing | 第8-18页 |
| 1.1 Background | 第8-14页 |
| 1.1.1 Name and classification | 第8-10页 |
| 1.1.2 Research ofbrachyurization | 第10-12页 |
| 1.1.3 Arthromere related genes | 第12-14页 |
| 1.2 RNA-Seq and DGE | 第14-18页 |
| 1.2.1 RNA-Seq and DGE technology | 第14-16页 |
| 1.2.2 Application of RNA-Seq and DGE | 第16-18页 |
| Chapter 2 Deep sequencing-based transcriptome and digital gene expressionprofiling analysis ofChinese mitten crab | 第18-46页 |
| 2.1 Introduction | 第18-19页 |
| 2.2 Meterials and methods | 第19-23页 |
| 2.2.1 RNA preparation for RNA-Seq | 第19-20页 |
| 2.2.2 Analysis of the transcriptome results | 第20-21页 |
| 2.2.3 DGE library construction and sequencing | 第21页 |
| 2.2.4 Mapping DGE tags to reference transcriptome databases | 第21-22页 |
| 2.2.5 Identification of differentially expressed genes | 第22-23页 |
| 2.2.6 Real-time RT-PCR analysis | 第23页 |
| 2.3 Results | 第23-42页 |
| 2.3.1 RNA preparation | 第23-24页 |
| 2.3.2 Assembly of E. sinensis transcriptome analysis | 第24-26页 |
| 2.3.3 Annotation of unigenes | 第26-27页 |
| 2.3.4 Gene ontology (GO) and clusters of orthologous groups (COG)classification | 第27-29页 |
| 2.3.5 Protein coding region prediction | 第29-32页 |
| 2.3.6 Digital gene expression library preparation and sequencing | 第32-36页 |
| 2.3.7 Mapping sequences to the reference transcriptome database | 第36-39页 |
| 2.3.8 Gene expression variations among the different developmental stages | 第39-41页 |
| 2.3.9 Real-time RT-PCR analysis | 第41-42页 |
| 2.4 Discussion | 第42-46页 |
| 2.4.1 Illumina sequencing and assembly | 第42-44页 |
| 2.4.2 Functional annotation of unigenes | 第44页 |
| 2.4.3 Differentially expressed genes | 第44-46页 |
| Chapter 3 Identification and characterization of two heat shock cognate 70 genes inthe Chinese mitten crab Eriocheir sinensis | 第46-78页 |
| 3.1 Introduction | 第46-48页 |
| 3.2 Material and methods | 第48-53页 |
| 3.2.1 Animal and sample preparation | 第48页 |
| 3.2.2 Total RNA isolation and reverse transcription polymerase chain reaction(RT-PCR) | 第48页 |
| 3.2.3 Use of RACE to obtain full-length cDNA sequences | 第48-50页 |
| 3.2.4 Sequences analyses ofEjsHSC70-5 and EjsHSC70-3 genes | 第50-52页 |
| 3.2.5 Multiple sequence alignment and phylogenetic analyses | 第52-53页 |
| 3.3 Results and Discussion | 第53-78页 |
| 3.3.1 Identification and characterization of full-length cDNAs encodingEsHSC70-5 and EsHSC70-3 | 第53-74页 |
| 3.3.2 Phylogenetic analysis using HSC70-5 and HSC70-3 sequences | 第74页 |
| 3.3.3 Structural analyses of EsHSC70-5 and EsHSC70-3 | 第74-78页 |
| Reference | 第78-91页 |
| Acknowledgements | 第91页 |
