| SUMMARY | 第1-8页 |
| 摘要 | 第8-10页 |
| INTRODUCTION | 第10-14页 |
| MATERIALS AND METHODS | 第14-20页 |
| Computational prediction of silkwom microRNAs | 第14页 |
| Silkworm culture and RNA extraction | 第14-15页 |
| Microarray printing and hybridization | 第15-16页 |
| Northern blotting | 第16-17页 |
| Cell culture and ecdysone treatment | 第17-18页 |
| Small RNA libraries preparation for sequencing | 第18页 |
| Computational analysis of sequencing data | 第18-20页 |
| RESULTS | 第20-42页 |
| Identification of B.mori miRNA genes computationally and experimentally | 第20-24页 |
| microRNAs break surface groups by groups during the embryo development | 第24-27页 |
| The expression profiles are characteristic of instar-molt rhythmicity during larval stages | 第27-31页 |
| Insight into the pupa metamorphosis on the base of miRNA profiling | 第31-35页 |
| MiRNAs profiling in multiple tissues/organs of fifth-instar day-3 larvae | 第35-38页 |
| MiRNAs profiling in different tissues from the late fifth instar to 24-hour pupa | 第38-40页 |
| MiRNAs respond to ecdysone in a dose-dependent manner | 第40-42页 |
| DISCUSSION | 第42-54页 |
| How many miRNAs exist in the silkworm? | 第42-43页 |
| The expression rhythms of miRNAs are changing with molting hormone in response to developmental timing | 第43-47页 |
| Coexpression of microRNAs in the silkworm | 第47-49页 |
| The steroid hormone and miRNAs might be cooperatedly responsible for the development of B.mori | 第49-50页 |
| What is underlying the expression rhythms of miRNAs and ecdysone during the development of silkworm? | 第50-54页 |
| CONCLUSION | 第54-56页 |
| INNOVATION OF THE RESEARCH | 第56-58页 |
| REFERENCES | 第58-69页 |
| SUPPLEMENTAL DATEA | 第69-178页 |
| PUBLISHED PAPER | 第178-180页 |
| ACKNOWLEDGEMENT | 第180页 |
