| 摘要 | 第1-11页 |
| ABSTRACT | 第11-14页 |
| Part Ⅰ Literature review | 第14-54页 |
| Chapter 1 Hypothalamic control of the somatotropic,thyrotropic and corticotropic axes | 第14-28页 |
| ·Introducton | 第14-15页 |
| ·Hypothalamic hormones that stimulate the release of GH | 第15-17页 |
| ·Thyrotropin-releasing hormone | 第17-18页 |
| ·Corticotropin-releasing hormone | 第18-20页 |
| ·Somatostatin | 第20-22页 |
| ·Ghrelin | 第22-23页 |
| ·Conclusions | 第23页 |
| References | 第23-28页 |
| Chapter 2 Biological function and nutritional regulation of the insulin-like growth factors system | 第28-54页 |
| ·Introduction | 第28-30页 |
| ·IGF-Ⅰ | 第30-31页 |
| ·IGF-Ⅱ | 第31-32页 |
| ·IGF-IR | 第32-33页 |
| ·IGFBP-2 | 第33-34页 |
| ·IGFBP-5 | 第34-36页 |
| ·IGFBP-7 | 第36-37页 |
| ·IGFBP-3 | 第37-38页 |
| ·Regulation of IGFs synthesis and release | 第38-40页 |
| ·Biological functions of IGFBPs | 第40-42页 |
| ·Summary of IGF and its binding proteins possible roles | 第42-44页 |
| ·Nutritional regulation of the insulin-like growth factors | 第44-45页 |
| References | 第45-54页 |
| Part Ⅱ Experimental research | 第54-139页 |
| Chapter 3 Expression of genes involved in the somatotropic,thyrotropic,and corticotropic axes during development of Langshan and Arbor Acres Chickens | 第54-85页 |
| ·Introduction | 第54-55页 |
| ·Materials and methods | 第55-60页 |
| ·Animals and tissues sampling | 第55-56页 |
| ·RNA extraction and cDNA synthesis | 第56-57页 |
| ·Establishment of standards of chicken genes for real-time Q-PCR | 第57-59页 |
| ·Real-time Q-PCR assay | 第59页 |
| ·Real-time Q-PCR data calculation | 第59页 |
| ·Statistical analysis | 第59-60页 |
| ·Results | 第60-72页 |
| ·Developmental changes of body weights of LS and AA chickens | 第60页 |
| ·Expression profiles of SS,Ghrelin,GHRH,GHSR1a,TRH and CRH during embryonic and postnatal hypothalamus development | 第60-63页 |
| ·Expression profiles of GH,GHRHR and TSHβ during embryonic and postnatal pituitary development | 第63-65页 |
| ·Expression profiles of IGF system genes and GHR during embryonic and postnatal liver development | 第65-67页 |
| ·Correlation and linear regression analysis between body weight,GH,TSHβ,IGFs and their respective correlative gene in chickens | 第67-72页 |
| ·Dicussion | 第72-79页 |
| ·Ontogenic changes of mRNA expression of genes | 第72-77页 |
| ·Effect of the diet on growth and gene expression | 第77-79页 |
| ·Conclusions | 第79-80页 |
| References | 第80-85页 |
| Chapter 4 Investigation of the insulin-like growth factor system in breast muscle during development in Langshan and Arbor Acres chickens | 第85-108页 |
| ·Introduction | 第85-86页 |
| ·Materials and methods | 第86-87页 |
| ·Animals and tissues sampling | 第86页 |
| ·RNA extraction and cDNA synthesis | 第86页 |
| ·Establishment of standards of chicken genes for real-time Q-PCR | 第86页 |
| ·Real-time Q-PCR assay | 第86页 |
| ·Real-time Q-PCR data calculation | 第86页 |
| ·Statistical analysis | 第86-87页 |
| ·Results | 第87-98页 |
| ·Developmental changes of general body growth in chickens | 第87-88页 |
| ·IGF system genes expression in the breast muscle of chickens | 第88-91页 |
| ·Effect of diet on strain differences in body growth and IGF system genes expression | 第91-93页 |
| ·Correlation between BMW and IGF system genes expression in chickens | 第93-95页 |
| ·Linear regression analysis of BMW and IGF system genes expression in chickens | 第95-98页 |
| ·Discussion | 第98-102页 |
| ·Expression of IGF-Ⅰ,IGF-Ⅱ and IGF-IR genes in the breast muscle tissue during development | 第98-99页 |
| ·Expression of IGFBP-2,IGFBP-5,IGFBP-7 and IGFBP-3 genes in the breast muscle tissue during development | 第99-102页 |
| ·Conclusions | 第102页 |
| References | 第102-108页 |
| Chapter 5 Role of the insulin-like growth factor system in epiphyseal cartilage on the development of Langshan and Arbor Acres chickens Gallus domesticus | 第108-139页 |
| ·Introduction | 第108-109页 |
| ·Materials and methods | 第109-110页 |
| ·Animals and tissues sampling | 第109-110页 |
| ·RNA extraction and cDNA synthesis | 第110页 |
| ·Establishment of standards of chicken genes for real-time Q-PCR | 第110页 |
| ·Real-time Q-PCR assay | 第110页 |
| ·Real-time Q-PCR data calculation | 第110页 |
| ·Statistical analysis | 第110页 |
| ·Results | 第110-123页 |
| ·Developmental changes of general body growth in chickens | 第110-112页 |
| ·Expression of IGF system genes in the epiphyseal cartilage of chickens | 第112-115页 |
| ·Effect of diet on strain differences in body growth and IGF system genes expression | 第115-117页 |
| ·Correlation between BW,TW and IGF system genes expression | 第117-119页 |
| ·Linear regression analysis of TW and IGF system genes expression | 第119-123页 |
| ·Discussion | 第123-131页 |
| ·Expression of IGF-Ⅰ,IGF-Ⅱ,and IGF-IR genes in the epiphyseal cartilage tissue during development | 第123-127页 |
| ·Expression of IGFBP-2,IGFBP-5,IGFBP-7,and IGFBP-3 genes in the epiphyseal cartilage tissue during development | 第127-131页 |
| ·Conclusions | 第131页 |
| References | 第131-139页 |
| Part Ⅲ Final conclusions | 第139-141页 |
| Part Ⅳ Innovation | 第141-142页 |
| Acknowledgments | 第142-143页 |
| Publications | 第143页 |
