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Rapamycin Protects Against Paraquat-induced Pulmonary Epithelial Mesenchymal Transition through Wnt/β-catenin Pathway

Abbreviations第5-6页
Abstract第6-7页
Ⅰ. Introduction第8-10页
Ⅱ. Methods第10-23页
    2.1. Reagents第10页
    2.2. Cell culture and treatment第10-13页
    2.3. RNA extraction, reverse description (RT) and Real-time PCR assay第13-16页
        Below is the procedure for Real Time PCR第14-16页
            Isolating Total RNA, Including miRNA, from Samples第14页
            Reverse Transcription of RNA第14-15页
            Generating a Standard Curve第15页
            Real-time PCR for Detection of miRNA第15-16页
    2.4. Western blot analysis第16-22页
    2.5. Fluorescent immunostaining第22页
    2.6. Statistical analysis第22-23页
Ⅲ. Results第23-32页
    3.1 Rapamycin alleviates paraquat-induced EMT in A549 and MRC-5 cells第23-25页
        3.1.1 The expression level of E-cadherin and α-SMA mRNA in A549 and MRC-5 cells第23-24页
        3.1.2 The expression level of e-cadherin and alpha-SMA parotein in A549 and MRC-5cells第24-25页
    3.2 PQ activates the Wnt signaling pathway in A549 and MRC-5 cells,and rapamycin inhibited the these effects of PQ第25-29页
        3.2.1 PQ activities the Wnt signaling pathway in A549and MRC-5 cells第25-26页
        3.2.2 Rapamycine inhibited the these effects that PQ activates the Wnt signaling pathwayin A549and MRC-5 cells第26-29页
    3.3 Activation of Wnt signaling pathway attenuates the inhibitory effects ofrapamycin on the PQ-induced EMT第29-32页
Ⅳ. Discussion第32-33页
Ⅴ. In conclusion第33-34页
Literature review第34-38页
    1.1. The current opinion of Paraquat induce PF第34-35页
    1.2. The role of EMT in PQ induce PF第35-36页
    1.3. The role of Wnt/beta-catenin pathway in PF/PQ induce PF第36-38页
Bibliography第38-44页
Published paper第44-45页
Acknowledgements第45-46页

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