| Abbreviations | 第5-6页 |
| Abstract | 第6-7页 |
| Ⅰ. Introduction | 第8-10页 |
| Ⅱ. Methods | 第10-23页 |
| 2.1. Reagents | 第10页 |
| 2.2. Cell culture and treatment | 第10-13页 |
| 2.3. RNA extraction, reverse description (RT) and Real-time PCR assay | 第13-16页 |
| Below is the procedure for Real Time PCR | 第14-16页 |
| Isolating Total RNA, Including miRNA, from Samples | 第14页 |
| Reverse Transcription of RNA | 第14-15页 |
| Generating a Standard Curve | 第15页 |
| Real-time PCR for Detection of miRNA | 第15-16页 |
| 2.4. Western blot analysis | 第16-22页 |
| 2.5. Fluorescent immunostaining | 第22页 |
| 2.6. Statistical analysis | 第22-23页 |
| Ⅲ. Results | 第23-32页 |
| 3.1 Rapamycin alleviates paraquat-induced EMT in A549 and MRC-5 cells | 第23-25页 |
| 3.1.1 The expression level of E-cadherin and α-SMA mRNA in A549 and MRC-5 cells | 第23-24页 |
| 3.1.2 The expression level of e-cadherin and alpha-SMA parotein in A549 and MRC-5cells | 第24-25页 |
| 3.2 PQ activates the Wnt signaling pathway in A549 and MRC-5 cells,and rapamycin inhibited the these effects of PQ | 第25-29页 |
| 3.2.1 PQ activities the Wnt signaling pathway in A549and MRC-5 cells | 第25-26页 |
| 3.2.2 Rapamycine inhibited the these effects that PQ activates the Wnt signaling pathwayin A549and MRC-5 cells | 第26-29页 |
| 3.3 Activation of Wnt signaling pathway attenuates the inhibitory effects ofrapamycin on the PQ-induced EMT | 第29-32页 |
| Ⅳ. Discussion | 第32-33页 |
| Ⅴ. In conclusion | 第33-34页 |
| Literature review | 第34-38页 |
| 1.1. The current opinion of Paraquat induce PF | 第34-35页 |
| 1.2. The role of EMT in PQ induce PF | 第35-36页 |
| 1.3. The role of Wnt/beta-catenin pathway in PF/PQ induce PF | 第36-38页 |
| Bibliography | 第38-44页 |
| Published paper | 第44-45页 |
| Acknowledgements | 第45-46页 |
