| 中文摘要 | 第4-6页 |
| Abstract | 第6-8页 |
| Part One: The effects of mi R-17-92 cluster in cancer screening | 第12-38页 |
| 1. Introduction | 第12-13页 |
| 1.1 Identify valuable biomarkers for cancer screening is crucial | 第12页 |
| 1.2 Clustered mi RNAs were more reliable in cancer detection | 第12页 |
| 1.3 mi R-17-92 cluster have predictive value in early cancer detection | 第12-13页 |
| 1.4 Purpose of the study | 第13页 |
| 2. Materials and Methods | 第13-16页 |
| 2.1 Search strategy | 第13-14页 |
| 2.2 Inclusion and exclusion strategy | 第14页 |
| 2.3 Quality assessment | 第14页 |
| 2.4 Data extraction | 第14-15页 |
| 2.5 Statistical analysis | 第15-16页 |
| 3. Results | 第16-33页 |
| 3.1 Search results and characteristics of includedstudies | 第16-23页 |
| 3.2 Quality assessment | 第23页 |
| 3.3 Pooled diagnostic accuracy | 第23-29页 |
| 3.4 Threshold effect and non-threshold effect | 第29-30页 |
| 3.5 Meta-regression and sub-group analyses | 第30页 |
| 3.6 Sensitivity analysis and publication bias | 第30-33页 |
| 4. Disscussion | 第33-37页 |
| 4.1 mi R-17-92 cluster can serve as a non-invasive biomarker to predict cancer risk | 第33-34页 |
| 4.2 Clustered mi RNAs have a better diagnostic accuracy than single mi RNAs | 第34页 |
| 4.3 Factors lead to heterogeneity | 第34-35页 |
| 4.4 Potential mechanism of mi R-17-92 cluster promotes tumorigenesis | 第35-36页 |
| 4.5 Members of mi R-17-92 cluster had their own regulatory mechanism in cancers | 第36页 |
| 4.6 Strength of our meta-analysis | 第36页 |
| 4.7 Limitations in our meta-analysis | 第36-37页 |
| 5. Conclusion | 第37-38页 |
| Part Two: Prognostic role of mi R-17-92 family members in cancers | 第38-60页 |
| 1. Introduction | 第38-39页 |
| 1.1 Identifying reliable biomarkers for cancer prognosis evaluation is valuable | 第38页 |
| 1.2 mi RNAs can act as prognostic biomarkers in clinic | 第38-39页 |
| 1.3 Members of mi R-17-92 family can be potential prognostic markers in human cancers | 第39页 |
| 1.4 Purpose of the study | 第39页 |
| 2. Materials and Methods | 第39-42页 |
| 2.1 Literature Search Strategies | 第40页 |
| 2.2 Inclusion and exclusion criteria | 第40页 |
| 2.3 Data extraction | 第40-41页 |
| 2.4 Quality assessment | 第41页 |
| 2.5 Statistical analysis | 第41-42页 |
| 3. Results | 第42-57页 |
| 3.1 Search results | 第42-51页 |
| 3.2 mi R-17-92 family members' expression and cancer OS rates | 第51-54页 |
| 3.3 mi R-17-92 family members' expression and cancer DFS rates | 第54-56页 |
| 3.4 mi R-17-92 family cluster expression and cancer PFS rates | 第56-57页 |
| 4 Discussion | 第57-59页 |
| 4.1 mi R-17-92 family can predict prognosis in cancer patients | 第57页 |
| 4.2 Results in other studies | 第57页 |
| 4.3 Ethnicity may play an important role in cancer patient prognosis | 第57-58页 |
| 4.4 mi R-17-92 family play different role in human cancers | 第58页 |
| 4.5 Potential mechanism of mi R-17-92 family members in cancer prognosis | 第58-59页 |
| 4.6 Advantages in our study | 第59页 |
| 4.7 Limitations in the study | 第59页 |
| 5. Conclusion | 第59-60页 |
| Part Three: Potential mechanisms of mi R-17-92 cluster in colorectal cancer cells | 第60-75页 |
| 1. Introduction | 第60-61页 |
| 1.1 Colorectal cancer has been a major public health problem worldwide | 第60页 |
| 1.2 MicroRNAs are believed to be an important factor of CRC development | 第60-61页 |
| 1.3 The relationship of mi R-19b expression and colorectal cancer risk | 第61页 |
| 1.4 Purpose of the study | 第61页 |
| 2. Materials and Methods | 第61-66页 |
| 2.1 Cell lines and primer sequence | 第61-62页 |
| 2.2 Main Reagents and instruments | 第62页 |
| 2.3 Cell cultures condition | 第62页 |
| 2.4 Cell passages | 第62-63页 |
| 2.5 Cell cryopreservation | 第63页 |
| 2.6 Cell counting | 第63-64页 |
| 2.7 RNA Extraction and Quantitative Real-Time PCR | 第64页 |
| 2.8 Cell transfection assay | 第64-65页 |
| 2.9 Cell proliferations assay | 第65页 |
| 2.10 Wound healing assays | 第65-66页 |
| 2.11 Bioinformatics tools | 第66页 |
| 2.12 Statistical analyses | 第66页 |
| 3. Results | 第66-72页 |
| 3.1 mi R-19b was upregulated in CRC cell lines | 第66-68页 |
| 3.2 The effect of cell transfection | 第68-69页 |
| 3.3 mi R-19b promote HCT116 cell proliferation | 第69-70页 |
| 3.4 mi R-19b have no effect on HCT116 cells migration | 第70-71页 |
| 3.5 Potential targets of mi R-19b | 第71-72页 |
| 4. Discussion | 第72-74页 |
| 4.1 mi RNAs play an important role in human cancers | 第72页 |
| 4.2 mi R-17-92 cluster act as oncogenes in human cancers | 第72-73页 |
| 4.3 mi R-19 is a crucial member of mi R-17-92 cluster | 第73页 |
| 4.4 mi R-19b plays an important role in colorectal cancer development | 第73页 |
| 4.5 mi R-19b may have no effect on cell migration | 第73-74页 |
| 4.6 Circ RNAs play a key role in regular mi R-19b expression | 第74页 |
| 5. Conclusion | 第74-75页 |
| 参考文献 | 第75-88页 |
| 攻读硕士期间研究成果 | 第88-89页 |
| 综述 | 第89-107页 |
| References | 第104-107页 |
| 致谢 | 第107-108页 |
