| Abstract | 第8-10页 |
| 摘要 | 第11-13页 |
| Abbreviations | 第13-15页 |
| Chapter 1 | 第15-62页 |
| 1.1 Review of literature | 第15-34页 |
| 1.1.1 General overview | 第15-16页 |
| 1.1.2 Biology of M. anguillicaudatus | 第16-19页 |
| 1.1.3 Genetics and fisheries management | 第19页 |
| 1.1.4 Population genetics | 第19-21页 |
| 1.1.5 Testing the basic assumptions for population genetic analysis | 第21-29页 |
| 1.1.6 Environmental perturbation and population genetics of fish | 第29-31页 |
| 1.1.7 Hydrological alterations and loach populations in central China | 第31-32页 |
| 1.1.8 Microsatellites as tool for population genetic study | 第32-34页 |
| 1.2 Objectives of the study | 第34页 |
| 1.3 Materials and methods | 第34-46页 |
| 1.3.1 Fish sampling | 第34-35页 |
| 1.3.2 The pairwise geographical distance between the sampling localities | 第35页 |
| 1.3.3 Flow cytometric screening for ploidy level | 第35-37页 |
| 1.3.4 DNA extraction of the fish samples | 第37-38页 |
| 1.3.5 DNA analysis through agarose gel electrophoresis | 第38-39页 |
| 1.3.6 Spectrophotometric analysis of DNA | 第39-40页 |
| 1.3.7 Screening of primers for polymorphism | 第40-42页 |
| 1.3.8 Amplification of microsatellite loci | 第42-43页 |
| 1.3.9 Polyacrylamide gel electrophoresis | 第43-44页 |
| 1.3.10 Silver staining | 第44-45页 |
| 1.3.11 Software and analytical packages | 第45页 |
| 1.3.12 Data analyses | 第45-46页 |
| 1.4 Results | 第46-57页 |
| 1.4.1 Genetic diversity | 第46-52页 |
| 1.4.2 Population genetic structure | 第52-57页 |
| 1.5 Discussion | 第57-62页 |
| 1.5.1 Genetic diversity in Chinese M. anguillicaudatus | 第57-58页 |
| 1.5.2 Population structure | 第58-60页 |
| 1.5.3 Habitat fragmentation and implications for M. anguillicaudatus | 第60-62页 |
| Chapter 2 | 第62-94页 |
| 2.1 Review of literature | 第62-71页 |
| 2.1.1 General overview | 第62-63页 |
| 2.1.2 Biology of E. bambusa | 第63-65页 |
| 2.1.3 Spawning of E. bambusa | 第65页 |
| 2.1.4 Interest to fisheries and research | 第65页 |
| 2.1.5 Artificial domestication | 第65-66页 |
| 2.1.6 Genetics and fisheries management | 第66页 |
| 2.1.7 Population genetics | 第66页 |
| 2.1.8 Testing the basic assumptions for population genetic analysis | 第66页 |
| 2.1.9 Environmental perturbation and populations genetics of fish | 第66页 |
| 2.1.10 Microsatellites as tool for population genetics study | 第66-67页 |
| 2.1.11 Molecular divergence and phylogeography of Asiatic cyprinids | 第67-69页 |
| 2.1.12 Mitochondrial DNA as marker for genetic and evolutionary studies | 第69-71页 |
| 2.2 Objectives of the study | 第71页 |
| 2.3 Materials and methods | 第71-77页 |
| 2.3.1 Fish sampling | 第71-72页 |
| 2.3.2 The pairwise geographical distance between the sampling localities | 第72-73页 |
| 2.3.3 DNA extraction of the fish samples | 第73页 |
| 2.3.4 DNA analysis through Agarose gel electrophoresis | 第73页 |
| 2.3.5 Spectrophotometric analysis of DNA | 第73页 |
| 2.3.6 Amplification, electrophoresis and analyses of microsatellite loci | 第73-75页 |
| 2.3.7 Amplification, sequencing and analyses of Cyt b sequences | 第75-77页 |
| 2.4 Results | 第77-89页 |
| 2.4.1 Results as inferred from microsatellite loci | 第77-83页 |
| 2.4.2 Results as inferred from Cyt b sequences analyses | 第83-89页 |
| 2.5 Discussion | 第89-94页 |
| 2.5.1 Microsatellite diversity in E. bambusa | 第89-90页 |
| 2.5.2 Mitochondrial diversity | 第90页 |
| 2.5.3 Population structure | 第90-91页 |
| 2.5.4 The hydrological alterations and E. bambusa spawning | 第91-92页 |
| 2.5.5 Divergent mtDNA lineages of E. bambusa | 第92-93页 |
| 2.5.6 Conservation of vulnerable populations | 第93-94页 |
| Conclusion and future implications | 第94-96页 |
| Literature cited | 第96-115页 |
| Curriculum vitae | 第115-118页 |
| Acknowledgements | 第118页 |
