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干露和产卵过程中皱纹盘鲍的耐受性和免疫应答研究

Abstract第8-9页
摘要第10-12页
CHAPTER 1. GENERAL INTRODUCTION第12-25页
    1.1. Biological overview of Pacific abalone第12-21页
        1.1.1. General characteristics, habitat and distribution第12-13页
        1.1.2. The circulation system and immunology system第13-16页
            1.1.2.1. Blood circulation第13-14页
            1.1.2.2. Blood chemistry baseline values第14-16页
            1.1.2.3. Immunology system第16页
        1.1.3. Respiration system第16-19页
            1.1.3.1. Aerobic respiration and anaerobic respiration第16-19页
        1.1.4. Reproductive system第19-21页
    1.2. Status abalone in China第21-25页
        1.2.1. China's abalone market and transportation第22-23页
        1.2.2. The effects of transport stress第23-25页
            Defining stress第23-24页
            The research purpose第24-25页
CHAPTER 2. PHYSIOLOGICAL ASSESSMENT OF LIVE PACIFICABALONEIN DIFFERENT TRANSPORT PACKAGING第25-41页
    2.1. Introduction第25-26页
    2.2. Methodologies第26-29页
        2.2.1. Animals sample第26页
        2.2.2. Equipment第26-27页
        2.2.3. Experimental design第27-28页
        2.2.4. Statistical analysis第28-29页
    2.3. Results第29-37页
        2.3.1. Survival rate第29-33页
        2.3.2. Weight loss rate第33-37页
    2.4. Discussion第37-41页
        2.4.1. Survival rate第37-39页
        2.4.2. Weight loss rate第39-41页
CHAPTER 3. HPLC FOR DETERMINATION ORGANIC ACIDS INANAEROBIC RESPIRATION IN PACIFIC ABALONE HEMOLYMPHDURING AIR EXPOSURE TRANSPORT第41-55页
    3.1. Introduction第41-43页
    3.2. Methodologies第43-46页
        3.2.1. Animals sample第43页
        3.2.2. Chemical reagents第43页
        3.2.3. Equipment第43-44页
        3.2.4. Experimental design第44页
        3.2.5. Methods第44-45页
        3.2.6. Data analysis第45-46页
    3.3. Results第46-52页
        3.3.1. Blood pyruvate and blood lactate in H. discus hannai hemolymph第46-52页
    3.4. Discussion第52-55页
CHAPTER 4. IMPACTS OF AIR EXPOSURE ON THE IMMUNERESPONSE IN HALIOTIS DISCUS HANNAI DURING SIMULATEDLIVE TRANSPORT第55-69页
    4.1. Introduction第55-56页
    4.2. Methodologies第56-59页
        4.2.1. Animals sample第56页
        4.2.2. Chemical reagents第56-57页
        4.2.3. Equipment第57页
        4.2.4. Methods第57-59页
            4.2.4.1. Light microscope第58页
            4.2.4.2. Total hemocyte count (THC)第58页
            4.2.4.3. Haemocyte Mortality第58-59页
            4.2.4.4. Reactive oxygen species (ROS)第59页
    4.3. Results第59-65页
        4.3.1. Light microscopy第59-61页
        4.3.2. Total hemocytes count (THC)第61页
        4.3.3. Haemocyte mortality第61页
        4.3.4. Reactive oxygen species (ROS)第61-65页
    4.4. Discussion第65-69页
        4.4.1. Light microscope第65-66页
        4.4.2. Immune response第66-69页
CHAPTER 5. MORPHOLOGICAL CHANGES IN GONADS ANDTHE IMMUNE REPONSE OF HALIOTIS DISCUS HANNAI DURINGSPAWNING PERIOD第69-86页
    Part 5.1. Gonad morphological第69-76页
        5.1.1. Introduction第69-72页
        5.1.2. Methodologies第72-73页
            5.1.2.1 Animals sample第72-73页
            5.1.2.2. Chemical reagents第73页
            5.1.2.3. Equipment第73页
        5.1.3. Results第73-76页
    Part 5.2. Effects of immune response during spawning period第76-86页
        5.2.1. Methodologies第76-79页
            5.2.1.1. Animals sample第76页
            5.2.1.2. Chemical reagents第76-77页
            5.2.1.3. Equipment第77页
            5.2.1.4. Methods第77-78页
                5.2.1.4.1. Total hemocyte count(THC)第77-78页
                5.2.1.4.2. Hemocyte Mortality第78页
                5.2.1.4.3. Reactive oxygen species第78页
            5.2.1.5. Statistical analyses第78-79页
        5.2.2. Results第79-82页
            5.2.2.1. Total hemocytes count第79页
            5.2.2.2. Hemocyte Mortality第79-80页
            5.2.2.3. Reactive oxygen species第80-82页
        5.2.3. Discussion第82-86页
            5.2.3.1. Morphological changes in gonads第82-84页
            5.2.3.2. Immune response第84-86页
CHAPTER 6. SUMMARY AND FUTURE RESEARCH第86-88页
    6.1. Summary第86-87页
    6.2. Future research第87-88页
References第88-111页
Appendix 1. Changes in muscle of H. discus hannai after exposure to airimmediately from 20 to 5 ℃ for live transport simulation第111-112页
Appendix 2. Changes in muscle of H. discus hannai after exposure to airimmediately from 20 to 10 ℃ for live transport simulation第112-113页
Appendix 3. Changes in muscle of H. discus hannai after exposure to airimmediately from 20 to 20 ℃ for live transport simulation第113-114页
Appendix 4. Changes in muscle of H. discus hannai after exposure to airimmediately from 20 to 28 ℃ for live transport simulation第114-115页
Appendix 5. Representative flow cytometry analysis scatter plot of fixedhemocytes from H. discus hannai. Size against internal complexity densityplot of hemocyte positive for SYBR Green I staining. Two populations ofhemocytes were distinguishable: hyalinoc第115-116页
Appendix 6. Determined hemocyte mortality by flow cytometry analysisof H. discus hannai hemocytes positive for propidium iodide (PI)第116-117页
Appendix 7. Determination of oxidative activity with DCEH-DA in eachhemocyte population by flow cytometry analysis第117-118页
Achievements第118-119页
Acknowledgement第119-120页
Biography第120页

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