| ABSTRACT | 第12-15页 |
| 摘要 | 第16-19页 |
| INTRODUCTION | 第19-23页 |
| CHAPTER-1 Review of literature | 第23-47页 |
| 1. Allylisothiocyanate | 第23-26页 |
| 1.1 Brief history | 第23页 |
| 1.2 Biosynthesis and biological functions | 第23页 |
| 1.3 Commercial and other applications | 第23-24页 |
| 1.4 Safety | 第24页 |
| 1.5 Mode of insecticidal action | 第24页 |
| 1.6 Pesticidal activity | 第24-26页 |
| 1.6.1 Insecticidal activity | 第24-26页 |
| 1.6.2 Fungicidal and bactericidal activities | 第26页 |
| 2. Other glucosinolate breakdown products | 第26-34页 |
| 2.1 Pesticidal activity | 第26-34页 |
| 2.1.1 Insecticidal and nematocidal activity | 第26-29页 |
| 2.1.2 Fungicidal and bactericidal activities | 第29-32页 |
| 2.1.3 Herbicidal activity | 第32-34页 |
| 3. Other photochemicals | 第34-44页 |
| 3.1 Mode of action | 第34-36页 |
| 3.2 Insecticidal activity | 第36-44页 |
| 4. Pesticides mixtures | 第44-47页 |
| 4.1 Background of mixture pesticides | 第44页 |
| 4.2 Benefits associated with pesticide mixtures | 第44-47页 |
| CHAPTER-Ⅱ Effect of AITC on stored and vegetable insects | 第47-64页 |
| 1. Introduction | 第47-49页 |
| 2. Materials and methods | 第49-52页 |
| 2.1 Test insects and rearing conditions | 第49-50页 |
| 2.2 Chemicals and insecticides | 第50页 |
| 2.3 Susceptibility of pest insects to AITC in EPPENDORF micro test tube | 第50-52页 |
| 2.3.1 AITC tested against major stored product insects | 第50-51页 |
| 2.3.2 AITC tested against serious vegetable insect larvae | 第51-52页 |
| 2.4 PH3 experiment | 第52页 |
| 2.5 Data analysis | 第52页 |
| 3. Results | 第52-60页 |
| 3.1 AITC fumigant activity on stored insects | 第52-58页 |
| 3.1.1 Fumigant activity of AITC dry application | 第55页 |
| 3.1.2 Fumigant activity of AITC wet-table application | 第55页 |
| 3.1.3 Results of probit analysis | 第55-58页 |
| 3.2 AITC fumigant activity on vegetable insect larvae | 第58-60页 |
| 4. Discussion | 第60-62页 |
| 5. Conclusions | 第62-64页 |
| CHAPTER-Ⅲ Effect of AITC formulations on P. xylostella,P. rapae and S. lituralarvae by spraying and leaf dipping methods | 第64-86页 |
| 1. Introduction | 第64-67页 |
| 2. Materials and methods | 第67-70页 |
| 2.1 Chemicals | 第67页 |
| 2.2 Collection and culturing of insects | 第67-68页 |
| 2.3 Toxicity bioassays | 第68-69页 |
| 2.4 Chlorpyrifos and cypermethrin bioassay | 第69页 |
| 2.5 Enzymes preparation | 第69页 |
| 2.6 Assay of AChE activity | 第69-70页 |
| 2.7 Assay of GST activity | 第70页 |
| 2.8 Total protein content assay | 第70页 |
| 2.9 Data analysis | 第70页 |
| 3. Results and discussion | 第70-85页 |
| 3.1 AITC bioassays on three insect larvae | 第70-79页 |
| 3.1.1 AITC spraying and dipping bioassay on P. rapae larvae | 第71-72页 |
| 3.1.2 AITC spraying and dipping bioassay on P. xylostella larvae | 第72-74页 |
| 3.1.3 AITC spraying on P. xylostella,P. rapae and S. litura larvae | 第74-79页 |
| 3.2 Enzymes activity bioassays | 第79-85页 |
| 3.2.1 Effect of AITC formulations in the protein contents | 第79页 |
| 3.2.2 Effect of AITC formulations in the enzymes activities | 第79-85页 |
| 4. Conclusions | 第85-86页 |
| CHAPTER-Ⅳ Effect of hong ju on stored and vegetable insects,and in the AChE actiyityand grain viability | 第86-105页 |
| 1. Introduction | 第86-88页 |
| 2. Materials and methods | 第88-92页 |
| 2.1 Insects cultures | 第88页 |
| 2.2 Chemicals | 第88-89页 |
| 2.3 Susceptibility bioassays against test insect pests | 第89-90页 |
| 2.3.1 Fumigant toxicity test | 第89页 |
| 2.3.2 Spray application test | 第89-90页 |
| 2.4 Seed germination test | 第90页 |
| 2.5 Enzyme assays | 第90-91页 |
| 2.5.1 Purification of acetylcholinesterase(AChE) | 第90-91页 |
| 2.5.2 Total protein content assay | 第91页 |
| 2.5.3 In vivo AChE activity assays | 第91页 |
| 2.6 Statistical analysis | 第91-92页 |
| 3. Results | 第92-99页 |
| 3.1 Hong ju fumigation bioassays | 第92-97页 |
| 3.1.1 Hong ju fumigant activity on maize | 第92-94页 |
| 3.1.2 Hong ju fumigant activity on rice | 第94-96页 |
| 3.1.3 Effect of Hong ju on maize and rice viability | 第96-97页 |
| 3.2 Hong ju spray application bioassays | 第97-98页 |
| 3.3 Effect of Hong ju in the protein concentration and AChE activity | 第98-99页 |
| 4. Discussion | 第99-103页 |
| 5. Conclusions | 第103-105页 |
| CHAPTER-Ⅴ Ultra-structural observations in the midgut and integument epithelial cellsof T. confusum and P. xylostella larvae | 第105-133页 |
| 1. Introducton | 第105-106页 |
| 2. Materials and methods | 第106-109页 |
| 2.1 Chemicals and culturing of the insects | 第106-107页 |
| 2.2 Dissection and processing for electron microscopy transmission | 第107-109页 |
| 2.2.1 T. confusum larvae | 第107-108页 |
| 2.2.2 P.xylostella larvae | 第108-109页 |
| 3. Results | 第109-127页 |
| 3.1 Results of light microscopy | 第109-110页 |
| 3.2 Results of electron microscopy | 第110-127页 |
| 3.2.1 AITC-treated T. confusum larvae | 第110-116页 |
| 3.2.1.1 The integument | 第110-112页 |
| 3.2.1.2 The midgut | 第112-116页 |
| 3.2.2 The integument and midgut of PH_3-treated T. confusum larvae | 第116-118页 |
| 3.2.3 Results of electron microscopy of P. xylostella larvae | 第118-127页 |
| 3.2.3.1 Histology and ultra-structure of the integument of untreated larvae | 第118-119页 |
| 3.2.3.2 Histology and ultra-structure of the integument of treated larvae | 第119-122页 |
| 3.2.3.3 Ultra-structure of the midgut of treated and unreated larvae | 第122-127页 |
| 4. Discussion | 第127-132页 |
| 5. Conclusions | 第132-133页 |
| REFERENCES | 第133-170页 |
| ACKNOWLEDGEMENTS | 第170-171页 |
| Publicaitons | 第171页 |
