| Acknowledgment | 第1-9页 |
| Academic publicaitons | 第9-10页 |
| List of tables | 第10-11页 |
| List of figures | 第11-13页 |
| ABSTRACT | 第13-16页 |
| 摘要 | 第16-22页 |
| CHAPTER 1 BACKGROUND AND LITERATURE SURVEY | 第22-50页 |
| §1.1 GENERAL BACKGROUND | 第22-28页 |
| §1.2 LITERATURE SURVEY | 第28-50页 |
| ·Aims and objectives | 第37-38页 |
| ·Chromium in the environment | 第38-48页 |
| ·Significance of the study | 第48-49页 |
| ·Justification of the research | 第49-50页 |
| Chapter 2 Enrichment, isolation, and characterization of Cr (Vl)-reducing bacteira | 第50-78页 |
| §2.1 INTRODUCTION | 第50-52页 |
| §2.2 MATERIAL AND METHODS | 第52-58页 |
| ·Enrichment media | 第53页 |
| ·Identification and characterization of the bacterial isolate | 第53-55页 |
| ·Determination of minimal inhibitory concentration of chromium(Ⅵ) | 第55页 |
| ·Effect of chromium(Ⅵ)concentration on cells growth | 第55-56页 |
| ·Effect of temperature and pH on the cells growth and chromium(Ⅵ) reduction | 第56页 |
| ·Time course of cells growth and chromium(Ⅵ) reduction | 第56-57页 |
| ·Chromium(Ⅵ)essay | 第57页 |
| ·Kinetics of chromium(Ⅵ)reduction | 第57-58页 |
| §2.3 RESULTS | 第58-72页 |
| ·Isolation of bacterial strain and growth conditions | 第58-59页 |
| ·Identification and characterization of the bacterial isolate | 第59-66页 |
| ·Effect of chromium (Ⅵ) concentration on cells growth and Cr(Ⅵ) removal | 第66-67页 |
| ·Effect of pH on cells growth and chromium(Ⅵ)removal | 第67页 |
| ·Effect of temperature on growth and chromium(Ⅵ)reduction | 第67-72页 |
| §2.4. DISCUSSION | 第72-77页 |
| §2.5 CONCLUSION | 第77-78页 |
| CHAPTER 3 ENZYMATIC CR (Ⅵ) REDUCTION BY CYTOPLASMIC ANDMEMBRANE FRACTIONS OF BACILLUS CEREUS STRAIN PF-1 | 第78-98页 |
| §3.1 INTRODUCTION | 第79-82页 |
| §3.2 MATERIAL AND METHODS | 第82-87页 |
| ·Experimental design for Chromium (Ⅵ) reduction | 第82-87页 |
| ·Effect of electron donors on growth and Cr (Ⅵ) reduction | 第84页 |
| ·Sub-cellular fractionation of Bacillus cereus strain Pf-1 for localization of chromate reductase activity | 第84-85页 |
| ·Generic enzyme essay | 第85-86页 |
| ·Evaluation of kinetic parameters for Cr (Ⅵ) removal by Bacillus cereus strain Pf-1 | 第86-87页 |
| §3.3 RESULTS | 第87-93页 |
| ·Bacterial growth versus chromate reductase production | 第87-88页 |
| ·Cell permeabilization assay | 第88-89页 |
| ·Effect of electron donors | 第89-91页 |
| ·Sub-cellular fractionation of B. cereus for the localization of chromate reductase activity | 第91-92页 |
| ·Evaluation of kinetic parameters | 第92-93页 |
| §3.4 DISCUSSION | 第93-96页 |
| §3.5 CONCLUSION | 第96-98页 |
| CHAPTER 4 BIOSORPTION POTENTIAL OF Cr (Ⅵ) IONS BY DRIEDBIOMASS OF BACILLUS CEREUS | 第98-139页 |
| §4.1 INTRODUCTION | 第99-112页 |
| ·Bacterial biosorbents | 第106-108页 |
| ·Bacterial structure | 第108-112页 |
| §4.2 MATERIAL AND METHODS | 第112-119页 |
| ·Biosorbent preparation | 第112页 |
| ·Preparation of chromium (Ⅵ) solution | 第112-113页 |
| ·Chromium (Ⅵ) biosorption experiments | 第113-119页 |
| §4.3 RESULTS | 第119-132页 |
| ·Characterization of Bacillus cereus biomass | 第119-120页 |
| ·Effects of biosorption conditions | 第120-127页 |
| ·Biosorption isotherms | 第127-130页 |
| ·Kinetics of biosorption | 第130-132页 |
| §4.4. DISCUSSION | 第132-138页 |
| §4.5 CONCLUSION | 第138-139页 |
| CHAPTER 5 SUMMARY,PERSPECTIVES,APPLICATIONS,ANDLIMITATIONS | 第139-146页 |
| REFERENCES | 第146-179页 |
