| 目录 | 第1-5页 |
| 摘要 | 第5-7页 |
| Abstract | 第7-10页 |
| Part Ⅰ Introduction | 第10-20页 |
| References | 第13-20页 |
| Part Ⅱ Main Research Works | 第20-64页 |
| Chapter 1. A Simple and Label-Free Electrochemical Biosensor for DNA DetectionBased on the Super-Sandwich Assay | 第20-36页 |
| 1. Introduction | 第20-21页 |
| 2. Experimental | 第21-23页 |
| ·Materials | 第22页 |
| ·Monolayer Preparation | 第22-23页 |
| ·Electrochemical Measurements | 第23页 |
| ·Gel Electrophoresis | 第23页 |
| 3. Results and Discussion | 第23-32页 |
| ·Difference Between the Super-Sandwich and Traditional-Sandwich Structures | 第24页 |
| ·Ionic Strength and Hybridization Time | 第24-25页 |
| ·EIS Based on Super-Sandwich DNA-Sensor | 第25-27页 |
| ·Demonstration of the Super-Sandwich Assay | 第27-30页 |
| ·Sensitivity and Selectivity | 第30-32页 |
| 4. Conclusion | 第32-33页 |
| References | 第33-36页 |
| Chapter 2. A Super-Sandwich Assay for DNA Detection based on Label-Free SignalDecreased Electrochemical Biosensor | 第36-50页 |
| 1. Introduction | 第36-38页 |
| 2. Experimental | 第38-40页 |
| ·Materials | 第38-39页 |
| ·Monolayer Preparation | 第39页 |
| ·Electrochemical Measurements | 第39-40页 |
| ·Gel Electrophoresis | 第40页 |
| 3. Rusult and Discussion | 第40-46页 |
| ·Demonstration of the Super-Sandwich Assay | 第40-42页 |
| ·Demonstration of the Super-Sandwich Assay | 第42-43页 |
| ·Discussion of the Concentration of the L-1 and the Super-Sandwich Assay | 第43-44页 |
| ·Discussion of the Ionic Strength of Na~+ in the Hybridization Buffer and Immobilization Buffer | 第44页 |
| ·Sensitivity and Selectivity | 第44-46页 |
| 4. Conclusion | 第46-47页 |
| References | 第47-50页 |
| Chapter 3. A New Type of Super-Sandwich Assay for DNA Detection Based onLabel-Free Electrochemical Biosensor | 第50-64页 |
| 1. Introduction | 第50-51页 |
| 2. Experimental | 第51-53页 |
| ·Materials | 第51-52页 |
| ·Monolayer Preparation | 第52-53页 |
| ·Electrochemical Measurements | 第53页 |
| ·Gel Electrophoresis | 第53页 |
| 3. Rusult and Discussion | 第53-59页 |
| ·The Study and Illustrator of the New Type of the Super-Sandwich Assay | 第53-55页 |
| ·The Study of the Part of C-2 Adsorbing and Hybridizing on the Modified Electrode | 第55-56页 |
| ·Demonstration of the Super-Sandwich Assay | 第56页 |
| ·Magnesium Ionic Strength and Remain Time of DNAActivity | 第56-58页 |
| ·Discussion of the Concentration of C-1 and C-2 | 第58页 |
| ·Sensitivity and Selectivity | 第58-59页 |
| 4. Conclusion | 第59-61页 |
| References | 第61-64页 |
| 致谢 | 第64-66页 |
| Appendix: Paper Published During the Graduate | 第66页 |
