| CONTENTS | 第5-8页 |
| LIST OF TABLE | 第8-9页 |
| LIST OF FIGURE | 第9-11页 |
| ABSTRACT | 第11页 |
| ABBREVIATIONS | 第12-13页 |
| CHAPTER Ⅰ INTRODUCTION | 第13-16页 |
| CHAPTER Ⅱ LITERATURE REVIEW | 第16-37页 |
| 2.1 Structural and function,metabolism, and advantage of glutamine | 第16-19页 |
| 2.1.1 The structure and function of glutamine | 第16-17页 |
| 2.1.2 Glutamine metabolism pathway in cell | 第17-19页 |
| 2.1.3 The advantage of glutamine supplementation | 第19页 |
| 2.2 Glutamine plays an important role in cell proliferation | 第19-20页 |
| 2.3 Glutamine deprivation induce apoptosis | 第20-22页 |
| 2.4 Glutamine is an anti_apoptotic agent | 第22页 |
| 2.5 mTOR activity requires simultaneous glutamine efflux | 第22-24页 |
| 2.6 Glutamine metabolism regulate mTORC1 | 第24-25页 |
| 2.7 Glutamine stimulates protein synthesis | 第25页 |
| 2.8 Bioinformaties analysis and application | 第25-30页 |
| 2.8.1 The historical an concept of bioinformatics | 第26-27页 |
| 2.8.2 The development of bioinformatics databases and organizing | 第27-29页 |
| 2.8.3 Quantitation of protein expression methods in bioinformatics analysis | 第29-30页 |
| 2.9 The principle analysis using iTRAQ method | 第30-32页 |
| 2.10 The basic concept of research methods in bioinformatics | 第32-37页 |
| 2.10.1 The Gene Ontology(GO)is by far the most widely used for bioinformatics | 第32-34页 |
| 2.10.1.1 Biological process | 第33页 |
| 2.10.1.2 Molecular function | 第33-34页 |
| 2.10.1.3 Cellular component | 第34页 |
| 2.10.2 Molecular pathways associated using KEGG pathway | 第34-37页 |
| CHAPTER Ⅲ MATERIAL AND METHODS | 第37-54页 |
| 3.1 MATERIAL | 第37-40页 |
| 3.1.1 Materials and Apparatus for cell culture | 第37-38页 |
| 3.1.2 Material for Western Blot | 第38-40页 |
| 3.1.2.1 SDS-PAGE formula | 第38页 |
| 3.1.2.2 Important test reagents | 第38-39页 |
| 3.1.2.3 Important apparatus used for Western Blot | 第39-40页 |
| 3.2 METHODS | 第40-54页 |
| 3.2.1 Experimental design of research | 第40-41页 |
| 3.2.2 Information analysis process | 第41-42页 |
| 3.2.3 Cell cultrure and treatment | 第42-44页 |
| 3.2.3.1 Subculture procedurs | 第42页 |
| 3.2.3.2 Cell treatment | 第42-43页 |
| 3.2.3.3 Cell lysis | 第43-44页 |
| 3.2.4 iTRAQ protein profiling | 第44-48页 |
| 3.2.4.1 Experimental procedure of iTRAQ | 第44-45页 |
| 3.2.4.2 Protein extraction process | 第45-46页 |
| 3.2.4.3 Measurement the concentration of protein | 第46页 |
| 3.2.4.4 SDS-PAGE | 第46页 |
| 3.2.4.5 Protein digestion | 第46页 |
| 3.2.4.6 iTRAQ labeling | 第46-47页 |
| 3.2.4.7 SCX-separation | 第47页 |
| 3.2.4.8 Combined with qualitative analysis of liquid.based QE | 第47-48页 |
| 3.2.5 The iTRAQ quantitative proteomics principle | 第48页 |
| 3.2.6 Sample protein information | 第48-49页 |
| 3.2.7 Experimental tag information of protein quantitation | 第49页 |
| 3.2.8 Bioinformatics analysis | 第49-52页 |
| 3.2.8.1 Raw Mass Spectrometry data | 第49-50页 |
| 3.2.8.2 The Database selected for proteins identification | 第50页 |
| 3.2.8.3 Mascot search engine | 第50页 |
| 3.2.8.4 Gene Ontology annotation analysis | 第50-51页 |
| 3.2.8.5 COG annotation analysis | 第51-52页 |
| 3.2.8.6 Metabolic pathway comment | 第52页 |
| 3.2.9 Validation by Western Blot in HepG2 Cells | 第52-54页 |
| CHAPTER Ⅳ RESULTS | 第54-71页 |
| 4.1 Differentially expressed of protein identification and quantitation | 第54页 |
| 4.2 Functional annotation analysis of identified protein | 第54-63页 |
| 4.2.1 Gene Ontology(GO)annotation analysis | 第57-59页 |
| 4.2.2 COG function classification | 第59-60页 |
| 4.2.3 Pathway enrichment analysis of differentially expressed protein | 第60-63页 |
| 4.3 Molecuar pathways associated of glutamine deprivation compared to resupplemented glutamine | 第63-64页 |
| 4.4 Cluster of differentially expressed protein | 第64-67页 |
| 4.5 Functional protein association network analysis | 第67-69页 |
| 4.6 Validation of change in protein level by Western blot analysis | 第69-71页 |
| DISCUSSION | 第71-73页 |
| CONCLUSION | 第73-74页 |
| REFERENCES | 第74-84页 |
| ACKNOWLEDGEMENTS | 第84页 |
