| 摘要 | 第3-4页 |
| Abstract | 第4-5页 |
| Chapter 1 Introduction | 第9-25页 |
| 1.1 Malate dehydrogenase gene families in plants | 第9-14页 |
| 1.1.1 Plant malate dehydrogenase gene families | 第9-10页 |
| 1.1.2 Plant malate dehydrogenase functions | 第10-13页 |
| 1.1.3 Research progresses of cotton malate dehydrogenase | 第13-14页 |
| 1.2 Cotton fibers | 第14-19页 |
| 1.2.1 Cotton fiber development | 第14-16页 |
| 1.2.2 Cotton fiber elongation mechanism | 第16-18页 |
| 1.2.3 Importance of malate dehydrogenase during fiber elongation | 第18-19页 |
| 1.3 Cotton genomes | 第19-23页 |
| 1.3.1 Molecular evolution of cotton genome | 第19-21页 |
| 1.3.2 Sequence date of cotton genome | 第21-23页 |
| 1.4 Research objectives and proposed significance | 第23-25页 |
| 1.4.1 Key problems | 第23页 |
| 1.4.2 Research purposes and contents | 第23-24页 |
| 1.4.3 Proposed significance | 第24-25页 |
| Chapter 2 Material and methods | 第25-35页 |
| 2.1 Biomaterial | 第25页 |
| 2.2 Bioinformatics analysis | 第25-27页 |
| 2.3 RACE PCR | 第27-28页 |
| 2.4 Real-time quantitative and semi-quantitative RT-PCR | 第28页 |
| 2.5 Subcellular localization analysis | 第28-30页 |
| 2.6 Analysis of protein expression | 第30-33页 |
| 2.7 MDH activity assay and determination of malate content | 第33页 |
| 2.8 Prokaryotic expression and purification of proteins | 第33-35页 |
| Chapter 3 Identification of cotton MDH gene family and their sequence features | 第35-50页 |
| 3.1 Introduction | 第35-36页 |
| 3.2 Identification of MDH gene family in G. raimondii and G. arboretum genomes | 第36-39页 |
| 3.3 Identification of MDH gene family in G. hirsutum genome | 第39-42页 |
| 3.4 Sequence characteristics of cotton MDH genes | 第42页 |
| 3.5 Conserved domain analysis of cotton MDH genes | 第42-49页 |
| 3.6 Summary | 第49-50页 |
| Chapter 4 Evolutionary relationship and expression profiles of MDH gene family | 第50-69页 |
| 4.1 Introduction | 第50-51页 |
| 4.2 Phylogenetic relationships of cotton MDH genes | 第51-57页 |
| 4.3 Chromosomal location and duplication analysis of cotton MDH genes | 第57-60页 |
| 4.4 Expression pattern of MDH genes in different tissues of allotetraploid cotton | 第60-64页 |
| 4.5 Putative regulatory cis-elements in MDH gene promoters | 第64-68页 |
| 4.6 Summary | 第68-69页 |
| Chapter 5 Molecular characterization of GhcMDH1 gene | 第69-81页 |
| 5.1 Introduction | 第69-70页 |
| 5.2 Molecular cloning of a GhcMDH1 gene | 第70-72页 |
| 5.3 GhcMDH1 sequence features and phylogenetic analysis | 第72-75页 |
| 5.4 Tissue expression and subcellular localization of GhcMDH1 | 第75-78页 |
| 5.5 MDH enzymatic activity and malate content in cotton fiber cells | 第78-79页 |
| 5.6 Summary | 第79-81页 |
| Chapter 6 Discussion and conclusions | 第81-90页 |
| 6.1 Discussion | 第81-87页 |
| 6.2 Main conclusions | 第87-88页 |
| 6.3 Future prospects | 第88-90页 |
| References | 第90-101页 |
| Acknowledgement | 第101-103页 |
| Appendix I | 第103-107页 |
| Appendix II | 第107-108页 |
| Personal resume and published papers | 第108页 |
