| 摘要 | 第1-11页 |
| ABSTRACT | 第11-18页 |
| 目录 | 第18-20页 |
| 缩略词表 | 第20-22页 |
| CHAPTER1 MESENCHYMAL STEM CELL,PRE-ECLAMPSIA AND MICRORNA | 第22-43页 |
| 1 MESENCHYMAL STEM CELL BACKGOUND | 第22-28页 |
| ·The definition of mesenchymal stem cell | 第22-23页 |
| ·The marker for mesenchymal stem cell | 第23页 |
| ·Mesenchymal stem cell in vivo | 第23页 |
| ·The isolation of mesenchymal stem cell | 第23-24页 |
| ·MSC interactions with lymphocytes in vitro | 第24-26页 |
| ·MSC interactions with lymphocytes in vivo | 第26-28页 |
| 2 THE ROLE OF THE IMMUNE SYSTEM IN PREECLAMPSIA | 第28-32页 |
| ·General Immunologic Aspects of Preecoampsia | 第28-29页 |
| ·Immunological models of preeclampsia | 第29-32页 |
| 3 MIR-181A WITH IMMUNITY AND CANCER | 第32-34页 |
| ·miR-181a and immune system | 第32-33页 |
| ·miR-181a and cancer | 第33-34页 |
| References | 第34-43页 |
| CHAPTER 2 A NOVEL WAY TO ISOLATE MSC FROM UMBILICAL CORDS | 第43-56页 |
| 1 INTRODUCTION | 第43-44页 |
| 2 MATERIALS AND METHODS | 第44-46页 |
| 3 RESULTS | 第46-51页 |
| ·Isolation and characterization of MSC derived from human umbilical cord tissue | 第46-48页 |
| ·UCMSC are capable of suppressing T cells proliferation | 第48-50页 |
| ·UCMSC secrete soluble mediators involved in their mediated-inhibition of T cells proliferation | 第50-51页 |
| 4 DISCUSSION | 第51-53页 |
| 5 REFERENCES | 第53-56页 |
| CHAPTER 3 MESENCHYMAL STEM CELLS AMELIORATE TH1-INDUCED PRE-ECLAMPSIA-LIKE MICE VIATHE SUPPRESSION OF TNF-A | 第56-66页 |
| 1 INTRODUCTION | 第56-57页 |
| 2 MATERIALS AND METHODS | 第57-59页 |
| 3 RESULTS | 第59-62页 |
| ·UCMSC-based therapy ameliorates Th1-induced PE in mice | 第59-61页 |
| ·UCMSC-based therapy reverses the cytokine abnormal in PE uterine and spleen Iymphocytes | 第61-62页 |
| 4 DISCUSSION | 第62-63页 |
| 5 REFERENCES | 第63-66页 |
| CHAPTER 4 THE ROLE OF MICRORNA-181A IN LOCAL IMMUNE BALANCE THROUGH REGULATINGPROLIFERATION AND IMMUNOSUPPRESSIVE PROPERTIES IN MESENCHYMAL STEM CELLS | 第66-93页 |
| 1 INTRODUCTION | 第66-67页 |
| 2 MATERIALS AND METHODS | 第67-70页 |
| 3 RESULTS | 第70-84页 |
| ·Increased expression of miR-181a in PE MSC | 第70-71页 |
| ·miR-181a prevents MSC proliferation | 第71-73页 |
| ·miR-181a and TGF-β signaling | 第73-75页 |
| ·miR-181a regulates TGF-β signaling through TGFBR1 and TGFBRAP1 | 第75-78页 |
| ·miR-181a enhanced IL6, VEGF and IDO expression in MSC | 第78-81页 |
| ·miR-181a regulates immunosuppressive properties of MSC through MAPK signaling pathway | 第81-83页 |
| ·miR-181a attenuates immunosuppressive properties of MSC in vitro | 第83-84页 |
| 4 DISCUSSION | 第84-88页 |
| 5 REFERENCES | 第88-93页 |
| CHAPTER 5 MIR-181A ATTENUATES MSC-BASED THERAPY AMELIORATED EXPERIMENTAL COLITISINDUCED BY DEXTRAN SULFATE SODIUM | 第93-99页 |
| 1 INTRODUCTION | 第93页 |
| 2 MATERIALS AND METHODS | 第93-95页 |
| 3 RESULTS | 第95-97页 |
| ·miR-181a attenuates MSC-based therapy ameliorated experimental colitis induced by dextran sulfate sodium | 第95-97页 |
| 4 DISCUSSION | 第97页 |
| 5 REFERENCE | 第97-99页 |
| CONCLUSION | 第99-100页 |
| 博士研究生期间发表及待发表论文列表 | 第100-102页 |
| 致谢 | 第102-103页 |
| 感谢以下基金的资助 | 第103-104页 |
