| Abbreviations | 第12-15页 |
| Abstract | 第15页 |
| General Introduction | 第16-18页 |
| CHAPTER-Ⅰ Literature Review | 第18-45页 |
| 1.1 Rice Genetics | 第18-19页 |
| 1.2 Rice Genome | 第19-23页 |
| 1.2.1 Importance of Rice Genome Sequence | 第20-21页 |
| 1.2.2 General Characteristics of Rice Genome | 第21-23页 |
| 1.2.2.1 Size and Repetitive DNA Content | 第21页 |
| 1.2.2.2 Genetic and Molecular Mapping | 第21-22页 |
| 1.2.2.3 Annotation and Database of the Rice Genome | 第22-23页 |
| 1.3 Genetic Engineering of Rice | 第23-31页 |
| 1.3.1 Gene Transformation in Rice | 第23页 |
| 1.3.2 Gene Transformation Technique | 第23-27页 |
| 1.3.2.1 Agrobacterium-mediated Gene Transfer | 第23-25页 |
| 1.3.2.2 Microprojectile Bombardment | 第25-26页 |
| 1.3.2.3 Another Techniques Using for Gene Transformation | 第26-27页 |
| 1.3.3 Potential Application of Rice Genetic Engineering | 第27-31页 |
| 1.3.3.1 Engineering of Rice for Biotic Stress Tolerance | 第27-28页 |
| 1.3.3.2 Engineering of Rice for Abiotic Stress Tolerance | 第28-30页 |
| 1.3.3.3 Engineering of Rice for Nutritional Quality | 第30-31页 |
| 1.4 Functional Genomics of Rice | 第31-34页 |
| 1.4.1 Gene Expression Profiling | 第31-32页 |
| 1.4.2 Discovery of Important Agronomic Genes | 第32-34页 |
| 1.5 Molecular Structure of FCA | 第34-40页 |
| 1.5.1 FCA Function | 第35-36页 |
| 1.5.2 FCA Auto-regulation | 第36-37页 |
| 1.5.3 Molecular Structure of FCA in Oryza sativa | 第37-40页 |
| 1.6 Programmed Cell Death | 第40-45页 |
| 1.6.1 Function of PCD | 第40页 |
| 1.6.2 Hypersensitive Response: a model for the control of PCD | 第40-41页 |
| 1.6.3 Mechanisms of PCD | 第41-42页 |
| 1.6.4 Lytic vacuole-initiating plant PCD | 第42-43页 |
| 1.6.5 Comparisons of developmental PCD in plants and other organisms | 第43-45页 |
| CHAPTER Ⅱ Function Ectopic-Expression of rFCA-RRM2 Control Seed Mass and Seed Weight in Transgenic Rice | 第45-62页 |
| 2.1 INTRODUCTION | 第45-46页 |
| 2.2 MATERIALS AND METHODS | 第46-48页 |
| 2.2.1 Construction of the Plasmid Vector pBY520 | 第46页 |
| 2.2.2 Transformation and Regeneration of Transformants | 第46-47页 |
| 2.2.3 Southern and Northern Blotting Hybridization Analysis | 第47页 |
| 2.2.4 Transcriptional Level Analysis of MADS-box Genes | 第47页 |
| 2.2.5 Histological Analysis | 第47页 |
| 2.2.6 Phenotype Analyses of Transgenic Rice | 第47-48页 |
| 2.2.7 Grain Measurements | 第48页 |
| 2.2.8 Statistical Analysis | 第48页 |
| 2.3 RESULTS | 第48-60页 |
| 2.3.1 Molecular and Morphological Analysis of Transgenic T0 Plants | 第48-54页 |
| 2.3.2 Molecular and Morphological Analysis of Transgenic T1 Progeny | 第54-58页 |
| 2.3.3 Histological Analysis of Transgenic Plants | 第58-59页 |
| 2.3.4 Expression Analysis of MADS-box Genes in Transgenic Plants | 第59-60页 |
| 2.4 DISCUSSION | 第60-62页 |
| CHAPTER Ⅲ Function Ectopic Expression of OsPDCD5 Gene Induces PCD in Transgenic Rice | 第62-76页 |
| 3.1 INTRODUCTION | 第62-63页 |
| 3.2 MATERIALS AND METHODS | 第63-66页 |
| 3.2.1 Plant Material and Callus Induction | 第63页 |
| 3.2.2 RNA Extraction and RT-PCR | 第63页 |
| 3.2.3 Cloning and Sequencing of cDNA of OsPDCD5 | 第63-64页 |
| 3.2.4 Construction of the Binary Plasmid Vector pCAMBIA1304 | 第64页 |
| 3.2.5 Transformation and Production of Transformed Plants | 第64页 |
| 3.2.6 DNA Isolation and Analysis | 第64-65页 |
| 3.2.7 Northern Blot Hybridization | 第65页 |
| 3.2.8 Protein Isolation | 第65页 |
| 3.2.9 Data Analysis | 第65-66页 |
| 3.3 RESULTS | 第66-74页 |
| 3.3.1 Sequence Analysis of OsPDCD5 | 第66-68页 |
| 3.3.2 Screening Analysis of Transgenic Plants | 第68页 |
| 3.3.3 Detection of PCD in Transgenic Rice Plants | 第68-71页 |
| 3.3.4 Effect of OsPDCD5 Activity on Developmental Growth of Transgenic Plants | 第71-72页 |
| 3.3.5 Effect of OsPDCD5 Transcriptional Level on Transgenic Plants Morphology | 第72-74页 |
| 3.4 DISCUSSION | 第74-76页 |
| GENERAL CONCLUSIONS | 第76-77页 |
| PUBLICATION | 第77-78页 |
| REFERENCES | 第78-88页 |
