| ABSTRACT | 第5页 |
| CHAPTER 1 GENERAL INTRODUCTION | 第7-26页 |
| 1.1 A BRIEF OVERVIEW OF STROKE AND ITS PHARMACOLOGICAL TREATMENT | 第7-12页 |
| 1.1.1 Pathophysiology of Stroke | 第7-8页 |
| 1.1.2 Pharmacology of stroke drugs | 第8-10页 |
| 1.1.3 A brief overview of traditional Chinese medicines used in the treatment of stroke | 第10-12页 |
| 1.2 THE NMDA RECEPTOR: STRUCTURE, FUNCTION, AND PHARMACOLOGY | 第12-15页 |
| 1.3 METHODS USED IN THE SCREENING FOR NMDA RECEPTOR ANTAGONIST ACTIVITY IN TRADITIONAL CHINESE STROKE DRUGS | 第15-26页 |
| 1.3.1 In vitro cytotoxicity assay | 第15-18页 |
| 1.3.2 Whole-cell Patch-Clamp | 第18-19页 |
| 1.3.3 NMR analysis | 第19-21页 |
| 1.3.4 Phytochemical methods for extraction, fractionation, and isolation of active ingredients in TCMs | 第21-26页 |
| CHAPTER 2 NMDA RECEPTOR ANTAGONIST ACTIVITY IN TRADITIONAL CHINESE STROKE MEDICINES | 第26-48页 |
| 2.1 HYPOTHESIS, EXPERIMENTAL DESIGN, RATIONALE | 第26-28页 |
| 2.2 MATERIALS AND METHODS | 第28-35页 |
| 2.2.1 Preparation of aqueous TCMs extracts | 第28-31页 |
| 2.2.2 Cytotoxicity assay | 第31-33页 |
| 2.2.3 Whole-cell patch-clamp technique | 第33-34页 |
| 2.2.4 Atomic absorption spectroscopy | 第34-35页 |
| 2.3 RESULTS | 第35-45页 |
| 2.3.1 TCM effects on NMDA-evoked Whole-cell Current | 第35-37页 |
| 2.3.2 NMDAR Antagonist Activity of Mg2+ in TCM Extracts | 第37-41页 |
| 2.3.3 NMDA-induced Cytotoxicity | 第41-45页 |
| 2.4 DISCUSSION | 第45-48页 |
| CHAPTER 3 NMDA RECEPTOR ANTAGONIST AND NEUROPROTECTIVE ACTIVITY IN GOUTENG | 第48-70页 |
| 3.1 INTRODUCTION | 第48-52页 |
| 3.2 MATERIALS AND METHODS | 第52-58页 |
| 3.2.1 Extract fractionation | 第52-53页 |
| 3.2.2 Bioactivity guided isolation of enp | 第53-57页 |
| 3.2.3 Bioassay used in guidance of isolation | 第57页 |
| 3.2.4 Structure determination | 第57-58页 |
| 3.3 RESULTS | 第58-67页 |
| 3.3.1 Activity guided fractionation and isolation | 第58-61页 |
| 3.3.2 Bioassay of some standard compounds | 第61-67页 |
| 3.4 DISCUSSION | 第67-70页 |
| CHAPTER 4. MAGNESIUM AS NMDA ANTAGONIST IN DANSHEN | 第70-82页 |
| 4.1 INTRODUCTION | 第70-73页 |
| 4.2 MATERIALS AND METHODS | 第73-76页 |
| 4.2.1 Extract fractionation | 第73-74页 |
| 4.2.2 Atomic absorption spectroscopy | 第74-75页 |
| 4.2.3 Cation-exchange chromatography | 第75页 |
| 4.2.4 Bioassay of activity fractions | 第75-76页 |
| 4.3 RESULTS | 第76-80页 |
| 4.3.1 Effect of Danshen on NMDA-evoked whole-cell current | 第76-77页 |
| 4.3.2 Removal of Mg~(2+) in extract of Danshen | 第77-78页 |
| 4.3.3 Effect of Danshen fraction with/without Mg~(2+) | 第78-80页 |
| 4.4 DISCUSSIONS | 第80-82页 |
| CHAPTER 5. FLAVONOIDS ISOLATED FROM HUANGQIN INTERACTING WITH THE PDZ2 DOMAIN OF PSD-95 | 第82-111页 |
| 5.1 INTRODUCTION | 第82-84页 |
| 5.2 MATERIALS AND METHODS | 第84-88页 |
| 5.2.1 Bioactivity guided extraction and isolation of active compounds | 第84-86页 |
| 5.2.2 Preparative purification of active compounds | 第86-87页 |
| 5.2.3 Structure determination of active compounds | 第87-88页 |
| 5.3 RESULTS | 第88-107页 |
| 5.3.1 Screening of components in Huangqin for binding activity to PDZ2 of PSD95 by NMR based chemical shift perturbation experiments | 第88-94页 |
| 5.3.2 Isolation and determination of active components under the activity guidance | 第94-97页 |
| 5.3.3 Structure determination of the active compounds | 第97-107页 |
| 5.4 DISCUSSIONS | 第107-111页 |
| REFERENCES | 第111-125页 |
| ABBREVIATIONS | 第125-126页 |
| ACKNOWLEDGEMENTS | 第126-129页 |
| SUMMARY | 第129-146页 |
