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水稻抗白叶枯基因聚合和抗倒伏QTL定位

摘要第6-8页
ABSTRACT第8-10页
LIST OF ABBREVIATIONS第15-22页
CHAPTER 1 INTRODUCTION第22-41页
    1.1 BACKGROUND第22-27页
        1.1.1 Distribution and Impact of Bacterial Blight disease of rice第23-24页
        1.1.2 Discovery and Classification of Bacterial Blight disease第24页
        1.1.3 Morphology and Physiology of Xanthomonas oryzae pv.oryzae第24-25页
        1.1.4 Races of Xanthomonas oryzae pv.oryzae (Xoo)第25页
        1.1.5 Mode of Infection第25-26页
        1.1.6 Mode of Symptom and Sign of Bacterial Blight disesase第26-27页
    1.2 DISEASE MANAGEMENT第27-32页
        1.2.1 Biological Control第27-28页
        1.2.2 Chemical Control第28页
        1.2.3 Genetic Resistance against Bacterial Blight disease第28-32页
    1.3 TYPES OF RESISTANCE第32-34页
        1.3.1 Durability of Resistance第32页
        1.3.2 Role of Conventional Breeding and Maker Assisted Selection第32-34页
        1.3.3 Transgenic Approach第34页
    1.4 SIGNIFICANCE OF STABILITY ANALYSIS第34-35页
    1.5 LODGING RESISTANCE第35-39页
        1.5.1 Types of Lodging第36-37页
        1.5.2 Yield Loss第37页
        1.5.3 Lodging Assessment第37-38页
        1.5.4 Improvement of Lodging Resistant第38-39页
    1.6 SCOPE AND OUTLINE OF THE THESIS第39-41页
CHAPTER 2 IDENTIFICATION OF RICE PYRAMIDING LINES IN RILs OFCIHERANG WITH IRBB60 AND F88 WITH IRBB60 CROSSES第41-79页
    2.1 SUMMARY第41页
    2.2 INTRODUCTION第41-44页
    2.3 PLANT MATERIALS第44-46页
    2.4 METHODS第46-52页
        2.4.1 Inoculum Preparation第46页
        2.4.2 BB Races Inoculation and Assessment第46-47页
        2.4.3 Preparation of CTAB and TAE buffer第47-48页
        2.4.4 DNA Isolation第48-49页
        2.4.5 DNA Quantification第49页
        2.4.6 Molecular markers used for detecting BB resistance genes in RILs第49-51页
        2.4.7 Gene linked primer for the Xa4 gene analysis第51页
        2.4.8 PCR analysis for the xa5 gene第51-52页
        2.4.9 Gene based and STS primers for the xa13 and Xa21 genes第52页
    2.5 GENOTYPING第52-56页
        2.5.1 SSR marker第52-53页
        2.5.2 Polymerase Chain Reaction (PCR)第53-54页
        2.5.3 Temperature profile第54页
        2.5.4 Polyacrylamide Gel Electrophoresis for SSR product analysis第54-55页
        2.5.5 Polymorphism study第55页
        2.5.6 Genotyping scoring第55-56页
    2.6 DATA COLLECTION第56页
    2.7 STATISTICAL ANALYSIS第56页
    2.8 RESULT第56-75页
        2.8.1 Reaction of RILs to BB races第56-59页
        2.8.2 Molecular markers analysis to identify pyramiding lines第59-67页
        2.8.3 Parental survey第67-72页
        2.8.4 Contribution of genome proportion derived from parents to pyramid lines第72-75页
    2.9 DISCUSSION第75-77页
    2.10 CONCLUSION第77-79页
CHAPTER 3 DETERMINATION OF ENVIRONMENTAL INFLUENCES ONPYRAMID RICE LINES第79-94页
    3.1 SUMMARY第79页
    3.2 INTRODUCTION第79-81页
    3.3 MATERIALS AND METHODS第81-84页
        3.3.1 Experimental sites第81页
        3.3.2 Plant materials第81-84页
        3.3.3 Data collection第84页
        3.3.4 Statistical analysis第84页
    3.4 RESULTS AND DISCUSSION第84-93页
    3.5 CONCLUSION第93-94页
CHAPTER 4 IDENTIFICATION OF LODGING RESISTANCE QTLs IN RICE RILS第94-127页
    4.1 SUMMARY第94-95页
    4.2 INTRODUCTION第95-97页
    4.3 MATERIALS AND METHODS第97-100页
        4.3.1 Plant materials第97页
        4.3.2 Phenotyping第97-99页
        4.3.3 Genotyping第99-100页
        4.3.4 Data analysis第100页
    4.4 RESULTS第100-123页
        4.4.1 Differences in parental lines and their RIL population第100-103页
        4.4.2 Correlation analysis among lodging resistant traits第103-104页
        4.4.3 QTLs affecting lodging traits第104-123页
    4.5 DISCUSSION第123-125页
    4.6 CONCLUSION第125-127页
REFERENCES第127-155页
APPENDICES第155-191页
ACKNOWLEDGEMENT第191-192页
RESUME第192页

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