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补充硼对非洲鸵鸟鸡脾、肾、肝发育的影响

List of abbreviations第12-15页
ABSTRACT (Chinese)第15-19页
ABSTRACT (English)第19-24页
CHAPTER1 INTRODUCTION第25-77页
    1.1 Occurrence, Source, and History of Boron第26-29页
    1.2 Requirement of Boron in Microorganisms and Plants第29-32页
    1.3 Role of Boron in Animals and Humans第32-50页
        1.3.1 Boron and Growth Performance第33页
        1.3.2 Boron and Meat Quality第33-35页
        1.3.3 Boron and Bone Development第35-37页
        1.3.4 Boron and Liver Functions第37-39页
        1.3.5 Boron and Embryonic Development第39-40页
        1.3.6 Boron and Brain Activity第40-41页
        1.3.7 Boron and Hormonal Effects第41-42页
        1.3.8 Boron and Wound Healing第42-44页
        1.3.9 Boron and Oxidative Stress第44-45页
        1.3.10 Boron and Anti-inflammatory or Immune Response第45-48页
        1.3.11 Boron and Cancer Treatment第48-50页
    1.4 Applications of Boron in Food and Medicinal sectors第50-53页
    1.5 Interaction of Boron with other Nutrients第53-54页
    1.6 Metabolic Effects of Boron第54-57页
    1.7 Pharmacokinetics of Boron第57-58页
    1.8 Mechanism of Action for the Bioactivity of Boron第58-60页
    1.9 Toxic Effects of Boron第60-62页
    1.10 Heat shock proteins (HSPs) and Spleen第62-73页
        1.10.1 Hsp40 Function and Mechanism第63-66页
        1.10.2 Hsp70 Function and Mechanism第66-67页
        1.10.3 General Mechanisms for Regulation of Hsp70 Function by Hsp40第67-70页
        1.10.4 HSPs as a Biomarker第70-73页
    1.11 Liver Morphology第73-75页
    1.12 Nrf2-ARE pathway in kidney第75页
    1.13 Objective of the study第75-77页
CHAPTER2 MATERIALS AND METHODS第77-109页
    2.1 Materials第77-79页
    2.2 Solutions used in study第79-83页
        2.2.1 Phosphate buffer saline (PBS)第79-80页
        2.2.2 Tris-buffered saline (TBS)第80页
        2.2.3 Citrate buffer第80-81页
        2.2.4 0.1 M Tris-HCl第81-82页
        2.2.5 Hematoxylin solution第82页
        2.2.6 Eosin solution第82-83页
    2.3 Experimental design第83-87页
    2.4 Methods第87-108页
        2.4.1 Animals, feeding, and sampling第87-88页
        2.4.2 Tissue fixation and paraffin embedding第88-90页
        2.4.3 Sectioning第90-91页
        2.4.4 HE staining第91-92页
        2.4.5 Immunohistochemistry (IHC)第92-94页
        2.4.6 Western blotting第94-101页
        2.4.7 Real-Time Quantitative PCR Analysis第101-103页
        2.4.8 Apoptosis detection第103-105页
        2.4.9 RNA-Sequencing analysis第105-108页
    2.5 Statistical analysis第108-109页
CHAPTER3 Effects of Boron supplementation on expression of Hsp70 in the Spleen of African ostrich第109-127页
    Introduction第109-111页
    Materials and Methods第111-114页
    Results第114-123页
    Discussion第123-127页
CHAPTER4 Boron affects the development of kidney through modulation of apoptosis, antioxidant capacity, and Nrf2 pathway in the African ostrich chicks第127-152页
    Introduction第127-129页
    Materials and Methods第129-133页
    Results第133-145页
    Discussion第145-152页
CHAPTER5 Morphological and Transcriptomic Analysis of the Supplemental Boron in the Liver of Ostrich Chicks第152-186页
    Introduction第152-153页
    Materials and Methods第153-157页
    Results第157-181页
    Discussion第181-186页
CHAPTER6 CONCLUSION&FUTURE PROSPECTS第186-189页
    Conclusion第186-188页
    Future prospects第188-189页
References第189-225页
APPENDIX第225-236页
RESEARCH ARTICLES第236-237页
ACKNOWLEDGEMENT第237页

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