| 附表 | 第5-6页 |
| ABSTRACT | 第6页 |
| 摘要 | 第7-11页 |
| List of abbreviations | 第11-12页 |
| CHAPTER 1.Research progress on the Riemerella anatipestifer | 第12-34页 |
| 1.1 INTRODUCTION | 第12-24页 |
| 1.1.1 The Riemerella anatipestifer genus'taxonomy | 第12页 |
| 1.1.2 Growth conditions and phenotypes of Riemerella anatipestifer | 第12-13页 |
| 1.1.3 Molecular biology and fingerprints of Riemerella anatipestifer | 第13页 |
| 1.1.4 Sequences analysis and virulence factors of Riemerella anatipestifer | 第13-14页 |
| 1.1.5 Serological Features | 第14页 |
| 1.1.6 Epidemiology of Riemerella anatipestifer | 第14-15页 |
| 1.1.7 Pathogenesis,clinical evidence and disease of Riemerella antipestifer infection | 第15-16页 |
| 1.1.8 Type IX secretion system overview | 第16-17页 |
| 1.1.9 Type IX secretion system | 第17-19页 |
| 1.1.10 Gliding motility of bacteriodetes | 第19-24页 |
| 1.2 Literature Review | 第24-33页 |
| 1.3 Goal of the project | 第33-34页 |
| 1.3.1 Research objectives | 第33页 |
| 1.3.2 Specific purpose of the project | 第33-34页 |
| CHAPTER 2 Riemerella anatipestifer AS87_08785 gene encodes a functional component GldK of the type IX secretion system | 第34-68页 |
| 2.1 Introduction | 第34-35页 |
| 2.2 Materials and methods | 第35-55页 |
| 2.2.1 Bacterial strains,plasmids and culture conditions | 第35页 |
| 2.2.2 Medium | 第35-36页 |
| 2.2.3 Buffer | 第36-37页 |
| 2.2.4 Equipment | 第37-38页 |
| 2.2.5 Consumables | 第38页 |
| 2.2.6 Chemical and reagents | 第38页 |
| 2.2.7 Enzymes | 第38页 |
| 2.2.8 Cloning kit | 第38-39页 |
| 2.2.9 Isolation of bacterial genomic DNA | 第39页 |
| 2.2.10 Concentration and quality of DNA determination | 第39-47页 |
| 2.2.11 Identification of the mutant strain Yb2ΔgldK | 第47页 |
| 2.2.12 Complementation of the Yb2ΔgldK mutant strain | 第47页 |
| 2.2.13 Bacterial growth,adherence and invasion assays | 第47-48页 |
| 2.2.14 GldK expression antibody production and western blotting analysis | 第48-49页 |
| 2.2.15 Subcellular fraction | 第49页 |
| 2.2.16 Western blot | 第49-50页 |
| 2.2.17 Microscopic observation of colony spreading | 第50页 |
| 2.2.18 Measurement of protease activity | 第50页 |
| 2.2.19 Liquid chromatography-tandem mass spectrometry(LC-MS/MS)analysis of the protein secretion | 第50-51页 |
| 2.2.20 RNA-sequencing and differential expression analysis | 第51页 |
| 2.2.21 Extraction of Bacterial Total RNA | 第51-52页 |
| 2.2.22 Real-time quantitative PCR(qPCR)analysis | 第52-54页 |
| 2.2.23 Assessment of virulence in vivo | 第54页 |
| 2.2.24 Statistical analysis | 第54-55页 |
| 2.3 RESULTS | 第55-65页 |
| 2.3.1 Bioinformatics analysis of the R.anatipestifer gldK gene | 第55-56页 |
| 2.3.2 Expression of R.anatipestifer recombinant GldK | 第56页 |
| 2.3.3 Identification of the mutant strain Yb2ΔgldK | 第56-58页 |
| 2.3.4 Deletion of the gldK gene decreases bacterial adherence and invasion capacities | 第58-59页 |
| 2.3.5 Subcellular location of the GldK | 第59-60页 |
| 2.3.6 The gldK deletion mutant is defective in gliding motility | 第60-61页 |
| 2.3.7 Deletion of the gldK gene significantly attenuates bacterial virulence | 第61-62页 |
| 2.3.8 The gldK deletion mutant exhibits defects in protein secretion | 第62-63页 |
| 2.3.9 The gldK disruption affects gene expression | 第63-65页 |
| 2.4 DISCUSSION AND CONCLUSION | 第65-68页 |
| REFERENCES | 第68-76页 |
| ACKNOWLEDGEMENTS | 第76-78页 |
| CURRICULUM VITAE | 第78-81页 |
