| 中文摘要 | 第1-14页 |
| 英文摘要 | 第14-18页 |
| 1. Review of Silicon Surface Functionalization | 第18-46页 |
| ·Fabricatation of molecular monolayers | 第18-29页 |
| ·Generation of surface Si-H_x(x=1,2,3) | 第18-20页 |
| ·Preparing terminated-carboxyl surface | 第20-23页 |
| ·Preparing terminated-hydroxyl surface | 第23-24页 |
| ·Preparing terminated-amino surface | 第24-25页 |
| ·Preparing terminal alkynes and azides on silicon surface | 第25-29页 |
| ·Immobilization of biomolecules on silicon surface | 第29-31页 |
| ·Preparation of grafted polymer brushes on silicon surface | 第31-38页 |
| ·Atomic transform radical polymerization technique | 第32-34页 |
| ·Applications of surface polymer brushes in protein immobilization | 第34-38页 |
| ·Construction of dendrite/dendron-modified silicon surface | 第38-40页 |
| ·The main aims of our work | 第40-41页 |
| References | 第41-46页 |
| 2. Enhanced Protein Loading on a Planar Si(111)-H Surface with Second GenerationNTA | 第46-59页 |
| ·introduciton | 第46-47页 |
| ·Experimental | 第47-50页 |
| ·Materials | 第47-48页 |
| ·Preparation of a second generation NTA on planar silicon surface | 第48-49页 |
| ·Instruments | 第49-50页 |
| ·Results and discussion | 第50-56页 |
| ·Preparation of UA-modified silicon surface and its esterification with NHS | 第50-52页 |
| ·Fabrication of First and second generation of NTA on silicon surface and its applications in protein anchoring | 第52-56页 |
| ·Conclusions | 第56-57页 |
| References | 第57-59页 |
| 3. Construction of Multiple Generation Nitriloacetates from Poly(PEGMA)Brushes onPlanar Silicon Surface for Enhancement of Protein Loading | 第59-82页 |
| ·Introduction | 第59-62页 |
| ·Experimental | 第62-65页 |
| ·Materials | 第62页 |
| ·Preparation of hydroxyl-and initiator-terminated surfaces | 第62-63页 |
| ·Grafting polymer brushes of Si-g-P(PEGMA) and converting their side-chains' terminal hydroxy #1 groups to carboxylic acids | 第63页 |
| ·Preparing first to third generation NHS and NTA brushes from Si-g-P(PEGMA-COOH) | 第63-64页 |
| ·Immobilization of his-tagged protein | 第64页 |
| ·Surface characterization | 第64-65页 |
| ·Results and discussion | 第65-75页 |
| ·Coupling UO onto Si(111)-H and introduction of surface initiators | 第65-66页 |
| ·Growth of Si-g-P(PEGMA) polymer brushes via ATRP and carboxylation of hydroxyl side chains | 第66-68页 |
| ·AFM images of UO-,initiator-,and polymer-brush-modified surfaces | 第68页 |
| ·Construction of first to third generation NHS ester(0.5G, 1.5G and 2.5G)and NTA(1.0G,2.0G and 3.0G)brushes | 第68-73页 |
| ·Anchoring his-tagged proteins | 第73-75页 |
| ·Conclusions | 第75-76页 |
| References | 第76-78页 |
| Supporting information | 第78-82页 |
| 4. Costructing Polyamidoamine Dendrons from Poly(poly(ethylene glycol)monomethacrylate) Brushes Grafting-from Planar Silicon Hydride Surfaces forBiomedical Applications | 第82-101页 |
| ·Introduction | 第82-85页 |
| ·Experimental | 第85-88页 |
| ·Materials | 第85页 |
| ·Preparation of Si-g-P(PEGMA-OH)brushes with Si-C links on silicon surfaces | 第85-86页 |
| ·Preparation of PAMAM dendrons from Si-g-P(PEGMA-OH) brushes | 第86页 |
| ·Application of 2.0G PAMAM dendrons for covalent binding of oxytocin | 第86页 |
| ·On-chip synthesis of a three amino acid peptide of Arg-Gly-Asp(RGD)from 3.0G | 第86-87页 |
| ·Instruments | 第87-88页 |
| ·Results and discussion | 第88-97页 |
| ·Conversion of hydroxyls of Si-g-P(PEGMA-OH)brushes into chlorines(Si-g-P(PEGMA-Cl)) | 第88-89页 |
| ·Conversion of chlorines of Si-g-P(PEGMA-Cl)into amines of 1.0G and subsequently to PAMAM dendrons of 2.0 and 3.0G | 第89-93页 |
| ·Activation of 1.0,2.0 and 3.0G with SMH and Anchoring of oxytocin | 第93-96页 |
| ·On-chip synthesis of RGD from terminal amino groups of 3.0G dendrons | 第96-97页 |
| ·Conclusions | 第97-98页 |
| References | 第98-101页 |
| 5. Microwave Irradiated Click Reactions on Silicon Surfaces via Derivertization ofCovalently Grafted Poly(PEGMA)Brushes | 第101-118页 |
| ·Introduction | 第101-103页 |
| ·Experimental | 第103-105页 |
| ·Materials | 第103页 |
| ·Preparing acetylene-and azide-functionalized silicon surfaces | 第103-105页 |
| ·Instruments | 第105页 |
| ·Results and Discussion | 第105-114页 |
| ·Conversion of terminal hydroxyls of Si-g-P(PEGMA-OH)to chlorines and subsequently to azides | 第105-108页 |
| ·Click reactions between Si-g-P(PEGMA-N_3) and acetylene-terminated compounds of propargylamine,propiolic acid and 10-undecynoic acid | 第108-112页 |
| ·Preparing acetylene-terminated surface(Si-g-P(PEGMA-CH_2C≡CH)) | 第112-114页 |
| ·Click reaction on Si-g-P(PEGMA-CH_2C≡CH)with benzyl azide | 第114页 |
| ·Conclusions | 第114-115页 |
| References | 第115-118页 |
| 6. Conclusions | 第118-120页 |
| 7. Publications | 第120-121页 |
| 8. 致谢 | 第121-122页 |
