| abstract | 第7-8页 |
| 摘要 | 第9-13页 |
| INTRODUCTION | 第13-15页 |
| Chapter 1: REVIEW OF LITERATURE WITH AMBITION OF EXPERIMENT | 第15-31页 |
| 1.1 Taxonomy and origin of pepper plant | 第15页 |
| 1.2 Nutritional and medicinal importance of pepper fruits | 第15-16页 |
| 1.3 Using ethyl methane sulphonate (EMS) as a tool for crop improvement | 第16-19页 |
| 1.3.1 Pepper | 第16-17页 |
| 1.3.2 Other crops | 第17-19页 |
| 1.4 kill curve analysis for determination of LD50, LD100 and survival ratio | 第19-24页 |
| 1.4.1 Germination percent and determination of LD50 and LD100 | 第19-22页 |
| 1.4.2 Survival ratio | 第22-24页 |
| 1.5 Effect of EMS on growth behaviors during M1 generation | 第24-26页 |
| 1.5.1 Pepper | 第24-25页 |
| 1.5.2 Other crops | 第25-26页 |
| 1.6 Observed changes or mutations during M2 generation | 第26-28页 |
| 1.6.1 Germination and survival ratios | 第26页 |
| 1.6.2 Abnormal observation during growth stages | 第26-28页 |
| 1.7 Molecular characterization of the yellow leaf color mutation | 第28-29页 |
| 1.8 Achieved goal and the importance of obtained results of current study | 第29-31页 |
| CHAPTER 2: MATERIALS AND METHODS | 第31-44页 |
| 2.1 Pepper cultivar source | 第31页 |
| 2.2 Mutagen (EMS) source and preparation | 第31页 |
| 2.2.1 Ethyl methane sulfonate (EMS) | 第31页 |
| 2.2.2 Preparation of mutagenic (EMS) solution: | 第31页 |
| 2.3 Kill curve analysis and LD50 determination | 第31-33页 |
| 2.3.1 Experimental procedures: | 第31页 |
| 2.3.2 Parameters studied: | 第31-33页 |
| 2.3.3 Statistical Analysis: | 第33页 |
| 2.4 First generation (M1) observations | 第33-35页 |
| 2.4.1 Parameter studied: | 第34页 |
| 2.4.2 Statistical analysis: | 第34-35页 |
| 2.5 Second generation observed mutations | 第35-36页 |
| 2.5.1 Parameters studied: | 第35-36页 |
| 2.5.2 Statistical analysis: | 第36页 |
| 2.6 Micro-propagation of M2 observed mutations | 第36页 |
| 2.6.1 Preparation and sterilization of explants | 第36页 |
| 2.6.2 Multiplication of M2 explants to obtain new plantlets | 第36页 |
| 2.7. Further observations during M3 generation | 第36-37页 |
| 2.7.1. Parameters studied: | 第36-37页 |
| 2.7.2. Statistical analysis | 第37页 |
| 2.8 Molecular analysis during M2 and M3 generations | 第37-44页 |
| 2.8.1 Primers design for CaBGL11 (DNA) gene during M2 generation | 第37页 |
| 2.8.2 Genomic DNA extraction of pepper (CTAB method) | 第37-38页 |
| 2.8.3 Polymerase Chain Reaction (PCR) conditions | 第38页 |
| 2.8.4 PCR-amplified DNA purification | 第38-39页 |
| 2.8.5 Ligation of Ca BGL11 gene with T-vector | 第39-40页 |
| 2.8.6 Isolation of plasmid | 第40页 |
| 2.8.7 Double digest | 第40-41页 |
| 2.8.8 Sequence analysis | 第41页 |
| 2.8.9 RNA extraction (Trizol method) during M2 and M3 generations | 第41-42页 |
| 2.8.10 Reverse transcription and cDNA synthesis in M2 and M3 generations | 第42页 |
| 2.8.11. Primers Design | 第42页 |
| 2.8.12. Determination of CaBGL11 gene expression in M2 generation and Ygl-7 gene in M3 generation | 第42-43页 |
| 2.8.13. Statistical analysis | 第43-44页 |
| CHAPTER 3: RESULTS | 第44-75页 |
| 3.1 Kill curve analysis and determination of LD50 | 第44-53页 |
| 3.1.1 Germination percentage (GP) and LD 50 | 第44页 |
| 3.1.2 Cumulative germination percent | 第44-45页 |
| 3.1.3 Seed vigor index (SVI) | 第45页 |
| 3.1.4 Growth characters | 第45-48页 |
| 3.1.5 Survival ratio (SR) | 第48页 |
| 3.1.6 Fruit characters | 第48页 |
| 3.1.7 Dwarfism | 第48-53页 |
| 3.2 First generation (M1) observed changes | 第53-59页 |
| 3.3 Second generation (M2) observed mutations | 第59-75页 |
| 3.3.1 Germination percent and survival ratio | 第59页 |
| 3.3.2 Screening of chlorophyll mutations during the seedling stage in M2 generation45 | 第59页 |
| 3.3.3 Characterization of specific M2 mutants | 第59-66页 |
| 3.3.4 Micro-propagation of observed mutations | 第66-68页 |
| 3.3.5 Further observations and Molecular characterization of the yellow green leaf color mutation in M2 and M3 generations | 第68-75页 |
| CHAPTER 4: DISCUSSION | 第75-85页 |
| 4.1 Kill curve analysis and determination of LD50 | 第75-79页 |
| 4.1.1 Germination percentage (GP) and LD 50 | 第75-76页 |
| 4.1.2 Cumulative germination percent | 第76页 |
| 4.1.3 Seed vigor index | 第76-77页 |
| 4.1.4 Seedling growth performance | 第77页 |
| 4.1.5 Survival ratio (SR) | 第77-78页 |
| 4.1.6 Fruit characteristics | 第78-79页 |
| 4.1.7 Dwarfism: | 第79页 |
| 4.2 First generation (M1) observed changes | 第79-81页 |
| 4.3 Second generation observed mutations | 第81-83页 |
| 4.4 Molecular characterization of the leaf color mutations in M2 generation | 第83页 |
| 4.5 Further observations and molecular characterization during M3 generation | 第83-85页 |
| CHAPTER 5: SUMMARY | 第85-87页 |
| CONCLUSION AND FUTURE PROSPECTIVE | 第87-88页 |
| ACKNOWLEDGEMENT | 第88-89页 |
| CHAPTER 6: REFERRANCES | 第89-104页 |
| APPENDIXES | 第104-111页 |
| Appendix A: Effect of different EMS concentrations and seed presoaking conditions ongermination percent and determination of LD50 after 7 days | 第104-105页 |
| Appendix B: Seed vigor index as affected by seed presoaking treatments and EMSconcentrations at 42 DAS | 第105-106页 |
| Appendix C: Effect of different EMS concentrations and seed presoaking conditions on plantheight at the maturity stage of the control plants | 第106-107页 |
| Appendix D: Effect of different EMS concentrations and seed presoaking conditions oninjury percent | 第107-108页 |
| Appendix E: Effect of different EMS concentrations and seed presoaking conditions onsurvival ratio | 第108-109页 |
| Appendix F: The used MS-media chemical composition | 第109-110页 |
| Appendix G: CTAB solution chemical composition | 第110页 |
| Appendix H: LB medium chemical composition | 第110-111页 |
| CURRICULUM VITAE | 第111-113页 |
