| Abstract | 第1-7页 |
| 摘要 | 第7-12页 |
| CHAPTER 1 Introduction and Literature Review | 第12-44页 |
| 1-Transient Receptor Potential(TRP) Ion Channel Receptors | 第12-44页 |
| ·-Historical Background of TRP Channel Receptors | 第15-19页 |
| ·-The trp Phenotype | 第15-17页 |
| ·-Activation of TRP ion channel receptors | 第17-19页 |
| ·-The TRP Channel Receptors Homologues in Different Species | 第19-41页 |
| ·-The "canonical" TRPCs | 第19-25页 |
| ·-General properties of TRPCs | 第19页 |
| ·-Regulation and binding partners of TRPCs | 第19-25页 |
| ·-The TRPV subfamily | 第25-31页 |
| ·-The TRPM subfamily | 第31-36页 |
| ·-The TRPML subfamily | 第36-37页 |
| ·-The TRPP subfamily | 第37-39页 |
| ·-The TRPA subfamily | 第39-41页 |
| ·-The TRPN Subfamily | 第41页 |
| ·Innate Immune Responses of Drosophila | 第41-44页 |
| CHAPTER 2 MATERIALS AND METHODS | 第44-57页 |
| ·-Fly strains | 第44页 |
| ·-Fly food and culture conditions | 第44-46页 |
| ·Material for fly food medium | 第44-45页 |
| ·-Procedure for food preparation | 第45页 |
| ·-Culturing Conditions | 第45-46页 |
| ·-Genetic Cross Schemes for imprecise P element excisions | 第46-48页 |
| ·-DNA work | 第48-49页 |
| ·Fly Genomic DNA preparation protocol used | 第48-49页 |
| ·-Polymerase chain reaction(PCR) | 第49-50页 |
| ·-Electrophoresis of DNA in agarose gel | 第50-51页 |
| ·-Purification of DNA from the gel for sequencing | 第51-52页 |
| ·-RNA preparations for RT-PCR | 第52-54页 |
| ·-Isolation of total RNA by TRIZOL Ls | 第52-53页 |
| ·-Reverse transcription by Reverse transcriptase M-MLV(RNase H-) | 第53-54页 |
| ·-Real time PCR | 第54-55页 |
| ·-Infection and Stress experiments | 第55-57页 |
| ·-Microbes and chemicals | 第55-57页 |
| CHAPTER 3 RESULTS AND DISCUSSION | 第57-76页 |
| ·Results | 第57-66页 |
| ·Generation of TRP receptors mutant flies by imprecise excision of Pelement. | 第57-58页 |
| ·Infection and Stress experiments | 第58-66页 |
| ·Infection experiments of fly strain CG31284 | 第58-61页 |
| ·Infection experiments of fly strain CG34123 | 第61-64页 |
| ·Stress experimentsof fly strain CG31284 | 第64-65页 |
| ·Stress experiments of fly strain CG34123 | 第65-66页 |
| ·mRNA expression levels of CG31284 and CG34123 mutants | 第66-68页 |
| ·Homologous protein alignment of TRP receptor with different species | 第68-74页 |
| ·Discussion | 第74-76页 |
| ACKNOWLEDGEMENTS | 第76-77页 |
| REFERENCES | 第77-84页 |
| LIST OF FIGURES AND TABLES | 第84-86页 |
| ABBREVIATIONS | 第86-87页 |
