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虾壳废弃物中蛋白与活性肽特性与综合利用研究

摘要第3-7页
ABSTRACT第7-8页
Chapter1 General introduction第13-25页
    1.1 Bioactive constituents from marine processing waste第13-16页
        1.1.1 Background第13页
        1.1.2 Proteins,peptides and amino acids from marine processing waste第13-14页
        1.1.3 Carbohydrates第14-15页
        1.1.4 Lipids and fatty acids第15页
        1.1.5 Antioxidants第15-16页
        1.1.6 Other constituents第16页
    1.2 Research progress of bioactive constituents of shrimp shell wastes(SSW)第16-19页
        1.2.1 Protein and peptide第17页
        1.2.2 Chitin and chitosan第17-18页
        1.2.3 Carotenoids第18页
        1.2.4 Fatty acids第18-19页
        1.2.5 The effects of processing treatment on the properties of SSW第19页
    1.3 Research progress of edible films第19-22页
        1.3.1 Edible films from proteins第20页
        1.3.2 Polysaccharides based coatings第20-21页
        1.3.3 Lipid based films第21页
        1.3.4 Composite films第21页
        1.3.5 Recent trends on the study of films第21-22页
    1.4 Significance of this study第22-25页
Chapter2 Biocatalytic conversion of shrimp shell wastes:optimization of hydrolytic conditions and theα-amylase inhibitory activity of the peptide hydrolysates第25-43页
    2.1 Introduction第25页
    2.2 Experimental materials,reagents and instruments第25-26页
    2.3 Methods第26-30页
        2.3.1 Enzymatic hydrolysis第26-27页
        2.3.2 The response surface methodology第27页
        2.3.3 Determination ofα-amylase inhibitory activities of shrimp shell wastes hydrolysates(SSWH)第27页
        2.3.4 Determination of antioxidant activities of SSWH第27-28页
        2.3.5 Amino acids compositional analysis of SSWH第28-29页
        2.3.6 Molecular weight distribution第29-30页
        2.3.7 Statistical analysis第30页
    2.4 Results and discussion第30-41页
        2.4.1 Optimization of the types of enzymes for hydrolysis of SSWH第30-31页
        2.4.2 Effect of hydrolysis pH on the α-amylase inhibitory activity of SSWH第31页
        2.4.3 Effect of hydrolysis time on theα-amylase inhibitory activity of SSWH第31页
        2.4.4 Effect of liquid-solid ratio on theα-amylase inhibitory activity of SSWH第31-32页
        2.4.5 Effect of enzyme concentration on theα-amylase inhibitory activity of SSWH第32页
        2.4.6 Effect of temperature on theα-amylase inhibitory activity of SSWH第32-34页
        2.4.7 The optimization of enzyme hydrolysis by response surface method第34-37页
        2.4.8 Antioxidant activities of SSWH obtained under the optimum conditions第37-39页
        2.4.9 The amino acids composition of SSWH obtained under the optimum conditions第39-40页
        2.4.10 Molecular weight distribution of SSWH obtained under the optimum conditions第40-41页
    2.5 Conclusion第41-43页
Chapter3 Effect of extrusion on physicochemical properties,functional properties and antioxidant activities of shrimp shell wastes protein第43-63页
    3.1 Introduction第43页
    3.2 Experimental materials,reagents and instruments第43-44页
    3.3 Methods第44-48页
        3.3.1 Extrusion process第44-45页
        3.3.2 Preparation of SSWP第45页
        3.3.3 Amino acids composition of SSWP第45页
        3.3.4 Morphological analysis of SSWP第45页
        3.3.5 Thermal properties of SSWP第45页
        3.3.6 Circular dichroism spectroscopy (CD) of SSWP第45-46页
        3.3.7 UV-vis spectroscopy of SSWP第46页
        3.3.8.1 Protein solubility of SSWP第46-47页
        3.3.9 Antioxidant activities of SSWP in vitro第47页
        3.3.10 Statistical analysis第47-48页
    3.4 Results and discussion第48-62页
        3.4.1 Effects of extrusion treatment on yields of SSWP第48-49页
        3.4.2 Amino acid composition of SSWP第49-50页
        3.4.3 Scanning electron microscopy of SSWP第50-52页
        3.4.4 Thermal properties of SSWP第52-54页
        3.4.5 Circular dichroism spectroscopy of SSWP第54-55页
        3.4.6 The UV spectra of SSWP第55-56页
        3.4.7 Functional properties of SSWP第56-59页
        3.4.8 Antioxidant activity of SSWP第59-61页
        3.4.9 Principal component analysis第61-62页
    3.5 Conclusion第62-63页
Chapter4 Physicochemical and antioxidant properties of shrimp shell waste protein-based films incorporated with different ethanol extracts for active packaging第63-77页
    4.1 Introduction第63页
    4.2 Experimental materials,reagents and instruments第63-64页
    4.3 Methods第64-67页
        4.3.1 Preparation of oolong tea extracts(OTE),corn silk extracts(CSE)and black soybean seed coat extract(BSSCE)第64-65页
        4.3.2 Extraction of SSWP第65页
        4.3.3 Film preparation第65页
        4.3.4 Characterization of SSWP-C-X films第65-66页
        4.3.5 Statistical analysis第66-67页
    4.4 Results and discussion第67-76页
        4.4.1 Film preparation and film thickness of SSWP-C-X films第67-68页
        4.4.2 Color of SSWP-C-X films第68-69页
        4.4.3 Light transmission and film transparency of SSWP-C-X films第69-71页
        4.4.4 Thermal properties of SSWP-C-X films第71-72页
        4.4.5 Microstructure of SSWP-C-X films第72-74页
        4.4.6 Antioxidant activity of SSWP-C-X films第74-76页
    4.5 Conclusion第76-77页
Chapter5 Conclusions and prospects第77-79页
References第79-95页
Achievements第95-97页
Acknowledgments第97页

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