| Table of Contents | 第1-5页 |
| Abstract | 第5-7页 |
| 中文摘要 | 第7-9页 |
| Abbreviations | 第9-11页 |
| Brief Review | 第11-51页 |
| 1. B cell development and maturation | 第13-20页 |
| ·From HSC to pro-B cell | 第13-15页 |
| ·From pro-B to pre-B cell | 第15-16页 |
| ·From Immature to naive B cell | 第16-17页 |
| ·B cell activation by antigen | 第17-20页 |
| 2. Primary and secondary immune response | 第20-32页 |
| ·Primary response | 第20-21页 |
| ·Germinal center formation | 第21-25页 |
| ·Somatic hypermutation | 第25-29页 |
| ·Cell survival selection in GC | 第29-30页 |
| ·Memory cell differentiation and response | 第30-32页 |
| 3. EAF family | 第32-36页 |
| ·EAF family members | 第32-33页 |
| ·About EAF2 | 第33-36页 |
| Referrence | 第36-51页 |
| Chapter Ⅰ Generation of mice deficient in the ELL-associated factor 2(Eaf2) | 第51-67页 |
| Introduction | 第53页 |
| Materials and Methods | 第53-57页 |
| Results | 第57-63页 |
| ·Overexpression of Eaf2 can induce B cell death | 第57-58页 |
| ·Generation of Eaf2~(-/-) mice | 第58-60页 |
| ·Normal B cell development,maturation and activation in Eaf2~(-/-) mice | 第60-61页 |
| ·Class switch and cell proliferation were normal in Eaf2~(-/-) mice | 第61-62页 |
| ·Normal levels of serum antibodies before immunization | 第62-63页 |
| Discussion | 第63-65页 |
| Reference | 第65-67页 |
| Chapter Ⅱ Increased germinal center B cell survival in Eaf2~(-/-) mouse | 第67-83页 |
| Introduction | 第69页 |
| Material and methods | 第69-71页 |
| Results | 第71-77页 |
| ·Increased proportion of B cells in the spleen and Peyer's patches of aged mice | 第71-72页 |
| ·Increased GC B cell survival in vitro | 第72-74页 |
| ·Enlarged GC size in Eaf2~(-/-) mice after immunization | 第74-75页 |
| ·Decreased TUNEL~+ cell in GC of Eaf2~(-/-) mice | 第75-77页 |
| Discussion | 第77-80页 |
| Reference | 第80-83页 |
| Chapter Ⅲ Humoral immune responses in Eaf2~(-/-) mice | 第83-103页 |
| Introduction | 第85页 |
| Materials and methods | 第85-88页 |
| Results | 第88-96页 |
| ·Increased numbers of GC and memory B cells at 2week after immunization | 第88-89页 |
| ·GC and memory cell decreased to normal level after 2 weeks | 第89-91页 |
| ·Increased NP-specific IgG1 production | 第91-92页 |
| ·Normal SHM and impaired antibody maturation | 第92-93页 |
| ·Normal memory response in Eaf2~(-/-) mice | 第93-96页 |
| Discussion | 第96-100页 |
| Reference | 第100-103页 |
| Acknowledgement | 第103-104页 |
| Publications | 第104-106页 |
