| 中文摘要 | 第1-7页 |
| Abstract | 第7-18页 |
| Preface | 第18-20页 |
| Chapter 1 Literature Review | 第20-43页 |
| ·Prevalence of HCC and its major causative agents | 第20-21页 |
| ·Molecular Pathogenesis of HCC | 第21-26页 |
| ·Molecular Pathogenesis of HCC related to viral infections and environmental Carcinogens | 第22-23页 |
| ·Molecular Pathogenesis of HCC related to genetic factors | 第23页 |
| ·Molecular Pathogenesis of HCC related to epigenetic factors | 第23-24页 |
| ·Molecular Pathogenesis of HCC related cellular signaling pathways | 第24-26页 |
| ·Treatment options and Novel Molecular targeted therapies in HCC | 第26-29页 |
| ·Small Leucine-rich Proteoglycan Families: General Structural Features | 第29-32页 |
| ·Decorin | 第32-34页 |
| ·Decorin in health and disease | 第34-35页 |
| ·Decorin as Anti-tumors | 第35-38页 |
| ·p53 tumor suppressor gene function | 第38-40页 |
| ·The TP53 Family | 第40-41页 |
| ·TP53 mutation in HCC | 第41-43页 |
| Chapter 2 Methodology | 第43-53页 |
| ·Research Design | 第43-44页 |
| ·Construction of recombinant human decorin gene | 第44-48页 |
| ·Digestion of cDNA decorin and pcDNA3.1plasmid by EcoRI and XbaI restriction enzymes | 第45页 |
| ·Ligation of decorin with pcDNA3.1 plasmid | 第45-46页 |
| ·Transformation of pcDNA3.1-decorin gene in to DH5-α competent E. coli and Cloning | 第46-47页 |
| ·Recombinant human decorin gene extraction from DH5-α E.coli | 第47-48页 |
| ·Cell Culture | 第48页 |
| ·Transient Transfection of HepG2 and Hep3B hepatoma cell lines | 第48-49页 |
| ·Recombinant human decorin gene expression Analysis | 第49页 |
| ·Cell viability Assay | 第49页 |
| ·Cell cycle Detection by Flow Cytometry | 第49-50页 |
| ·Apoptosis Analysis by Annexin V-FITC/PI Assay | 第50页 |
| ·RNA extraction | 第50页 |
| ·Complementary DNA (cDNA) Synthesis | 第50-51页 |
| ·Quantitative Real Time – PCR | 第51页 |
| ·Relative Quantification of mRNA | 第51-52页 |
| ·Statistical Analysis | 第52-53页 |
| Chapter 3: Result | 第53-66页 |
| ·Recombinant human decorin gene sequencing result | 第53-56页 |
| ·Recombinant human decorin gene expression on HepG2 and Hep3B cell lines | 第56页 |
| ·Recombinant human decorin gene transfection inhibits cell growth in HepG2 and Hep3B hepatoma cell lines | 第56-58页 |
| ·Recombinant human decorin gene transfection induces G0/G1 cell cycle arrest in HepG2 and Hep3B cells | 第58-60页 |
| ·Recombinant human decorin gene transfection induces apoptosis in HepG2 and Hep3B cells | 第60-63页 |
| ·P57KIP2expression in three group of HepG2 and Hep3B cells | 第63-66页 |
| Chapter 4 Discussion | 第66-74页 |
| ·Decorin and p53 | 第68-69页 |
| ·Recombinant human decorin gene transfer induced apoptosis in p53 wild-type HepG2 and p53-null Hep3B cells via Caspase-3 and Bcl-2 apoptoticpathway | 第69-70页 |
| ·Recombinant human decorin gene transfer up-regulated p57~(KIP2) expression in HepG2 and Hep3B hepatoma cell lines | 第70-74页 |
| Chapter 5 Conclusion | 第74-78页 |
| ·Summary | 第74-76页 |
| ·Future perspective | 第76-78页 |
| Reference | 第78-98页 |
| Novel Findings of the Study | 第98-99页 |
| List of SCI publications | 第99-100页 |
| ACKNOWLEDGEMENTS | 第100-101页 |
