| 摘要 | 第1-6页 |
| ABSTRACT | 第6-8页 |
| Table of Content | 第8-10页 |
| Chapter 1 Introductions | 第10-30页 |
| ·Biomining: historical development, current state and future | 第10-16页 |
| ·Historical development of biomining | 第10-12页 |
| ·Current state of biomining | 第12-15页 |
| ·Future of biomining | 第15-16页 |
| ·Microorganisms used for biomining | 第16-21页 |
| ·Mesophilic microorganisms | 第18-20页 |
| ·Thermophilic microorganisms | 第20-21页 |
| ·Hyperthermophilic microorganisms | 第21页 |
| ·Bioleaching of sulfide minerals with emphasis on chalcopyrite | 第21-28页 |
| ·Mechanisms of bioleaching | 第22-24页 |
| ·Sulfur chemistry | 第24-25页 |
| ·Iron chemistry | 第25-26页 |
| ·Microbial attachment | 第26-28页 |
| ·The objective and main content of the present study | 第28-30页 |
| ·The objective of the present study | 第28-29页 |
| ·The main content of the present study | 第29-30页 |
| Chapter 2 Isolation and Characterization of acidophilic sulfur-oxidizing bacteria | 第30-41页 |
| ·Material and method | 第30-32页 |
| ·Culture media | 第30页 |
| ·Isolation and morphology observation | 第30-31页 |
| ·Physiological characterization | 第31页 |
| ·Amplification, sequencing and phylogenetic analysis of 16S rRNA genes | 第31-32页 |
| ·Isolation and morphological characterization | 第32-33页 |
| ·Biochemical and physiological characterization | 第33-38页 |
| ·Biochemical and physiological characterization of Strain S_2 | 第33-34页 |
| ·Biochemical and physiological characterization of Strain A01, A02 and DMC | 第34-38页 |
| ·16S rRNA gene sequence analysis and identification of the strains | 第38-39页 |
| ·Conclusions | 第39-41页 |
| Chapter 3 The role of Acidithiobacillus caldus in bioleaching of chalcopyrite | 第41-49页 |
| ·Material and method | 第41-42页 |
| ·Microorganisms and minerals | 第41页 |
| ·Bioleaching experiments | 第41-42页 |
| ·Chemical analysis | 第42页 |
| ·Chalcopyrite bioleaching by Acidithiobacillus caldus and iron-oxidizing bacteria | 第42-45页 |
| ·SEM, EDX and XRD analysis of leached residues | 第45-47页 |
| ·Conclusions | 第47-49页 |
| Chapter 4 Bioleaching of chalcopyrite by Acidithiobacillus spp. and Leptospirillum ferriphilum | 第49-61页 |
| ·Materials and methods | 第49-50页 |
| ·Culture media and microorganisms | 第49页 |
| ·Minerals | 第49页 |
| ·Bioleaching experiments | 第49-50页 |
| ·Chemical analysis | 第50页 |
| ·Bioleaching of chalcopyrite by pure and mixed culture of Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans and Acidithiobacillus caldus | 第50-52页 |
| ·Bioleaching of chalcopyrite by pure and mixed culture of Leptospirillum ferriphilum and Acidithiobacillus caldus | 第52-55页 |
| ·SEM and AFM images of polished mineral blocks | 第55-57页 |
| ·SEM images of polished mineral blocks | 第55-56页 |
| ·AFM images of polished mineral blocks | 第56-57页 |
| ·The weigh ratio of jarosite and oxidized chalcopyrite in the leached residues from different bioleaching systems | 第57-59页 |
| ·Conclusions | 第59-61页 |
| Chapter 5 Conclusions and Future research Questions | 第61-64页 |
| ·Conclusions | 第61-62页 |
| ·Future research Questions | 第62-64页 |
| References | 第64-71页 |
| Acknowledgements | 第71-72页 |
| Main achievements | 第72页 |
