| 摘要 | 第1-6页 |
| ABSTRACT | 第6-8页 |
| Content | 第8-10页 |
| Chapter 1 Introductions | 第10-38页 |
| ·Structure,function,and formation of biological iron-sulfur clusters | 第10-24页 |
| ·Functions of Biological[Fe-S]Clusters | 第10-13页 |
| ·Structures and Properties of Biological[Fe-S]Clusters | 第13-17页 |
| ·Formation of Biological[Fe-S]Clusters | 第17-23页 |
| ·Structure and function of IscU | 第23-24页 |
| ·Structure,function,and secretion of signal peptide | 第24-36页 |
| ·Function of signal peptide | 第25-26页 |
| ·Structure of signal peptide | 第26-27页 |
| ·Classification of signal peptide | 第27-30页 |
| ·The secretion systems of signal peptides | 第30-35页 |
| ·Research methods of signal peptide | 第35-36页 |
| ·The objective and main content of the present study | 第36-38页 |
| ·The objective of the present study | 第36-37页 |
| ·The main content of the present study | 第37-38页 |
| Chapter 2 Expression,purification and characterization of an iron-sulfur cluster assembly protein IscU from A.ferrooxidans | 第38-51页 |
| ·Materials | 第38页 |
| ·Methods | 第38-42页 |
| ·Cloning of the IscU gene from A.ferrooxidans | 第38-39页 |
| ·Construction of A.ferrooxidans IscU mutant plasmids | 第39-40页 |
| ·Expression of the IscU and the mutant proteins | 第40页 |
| ·Purification of the IscU and the mutant proteins | 第40-41页 |
| ·UV-Vis Scanning and EPR spectra | 第41页 |
| ·Iron-sulfur cluster assembly in apoIscU in vitro | 第41页 |
| ·Molecular structure modelling of IscU | 第41-42页 |
| ·Results and discussion | 第42-50页 |
| ·Cloning of the IscU gene from A.ferrooxidans and construction of the mutant plasmids | 第42页 |
| ·Expression and purification of IscU and its mutant proteins | 第42-43页 |
| ·Iron-sulfur cluster assembly in IscU in vitro | 第43-46页 |
| ·The conserved residues for iron-sulfur cluster binding | 第46-48页 |
| ·Molecular Modelling of IscU from A.ferrooxidans | 第48-50页 |
| ·Conclusions | 第50-51页 |
| Chapter 3 Analysis of the secreted proteins encoded by genes in genome of A.ferrooxidans | 第51-74页 |
| ·Materials | 第51页 |
| ·Methods | 第51-53页 |
| ·Predict the proteins with secretory(See-type)signal peptides by SignalP and TMHMM | 第51-52页 |
| ·Predict the proteins Twin-arginine signal peptides by TatP and TMHMM | 第52页 |
| ·Predict the lipoprotein by LipoP and TMHMM | 第52页 |
| ·Predict the non-classically secreted proteins by SecretomeP,SignalP and TMHMM | 第52-53页 |
| ·Results and discussion | 第53-72页 |
| ·The See-type signal peptides | 第55-62页 |
| ·The proteins with Twin-arginine signal peptides | 第62-64页 |
| ·The lipoprotein with lipoprotein signal peptides | 第64-65页 |
| ·The non-classically secreted proteins | 第65-71页 |
| ·Discussion | 第71-72页 |
| ·Conclusions | 第72-74页 |
| Chapter 4 Conclusions | 第74-75页 |
| Reference | 第75-86页 |
| Acknowledgements | 第86-87页 |
| Main achievements | 第87页 |
