| Introduction | 第1-6页 |
| 摘要 | 第6-8页 |
| Abstract | 第8-13页 |
| Part Ⅰ Literature review | 第13-25页 |
| ·Biogenesis and Functions of microRNAs | 第13-17页 |
| ·Genomic organization and function of the miR-17～92 cluster | 第17-21页 |
| ·The regulation of the miR-17～92 cluster | 第21-25页 |
| ·c-Myc regulates the expression of miR-17～92 cluster | 第22-23页 |
| ·E2F family regulate the expression of miR-17～92 cluster | 第23页 |
| ·p53 and STAT3 | 第23-25页 |
| Part Ⅱ Experiment Report | 第25-91页 |
| ·Introduction | 第25页 |
| ·Materials and Methods | 第25-57页 |
| ·cells | 第26-27页 |
| ·miRNA profiling using the TaqMan(?) microRNA assays | 第27页 |
| ·Reverse transcriptase PCR (RT-PCR) | 第27-28页 |
| ·Real-time quantitative PCR | 第28-29页 |
| ·Plasmids | 第29-32页 |
| ·Overlap extension PCR | 第32-36页 |
| ·Luciferase assay | 第36页 |
| ·Cell transfection using TurboFectTM method | 第36-37页 |
| ·Transiently transfected using an Amaxa Nucleofector | 第37-38页 |
| ·Dnase Ⅰ hypersensitivity analysis | 第38-42页 |
| ·ChIP-on-chip | 第42-44页 |
| ·Chromatin Immunprecipation (ChIP) | 第44-54页 |
| ·The standad method | 第44-47页 |
| ·The method of ChampionChIPTM One-Day kit | 第47-54页 |
| ·Immunoblotting | 第54-57页 |
| ·Results | 第57-83页 |
| ·Full promoter activity requires sequences extending approximately from -188 nt to+115 nt from the transcriptional start site | 第57-58页 |
| ·The promoter is regulated by the collaborative activity of several transcription factors, each of which individually has only a moderate effect | 第58-62页 |
| ·The C13orf25 gene highly conserved in vertebrates | 第58-59页 |
| ·The study of several putative transcription factors binding sites in the promoter region of C13orf25 gene | 第59-62页 |
| ·Mxi1 binds to the promoter | 第62-68页 |
| ·miR-17～92 cluster expression profiling in B-cells | 第68-69页 |
| ·Histone modifications in C13orf25 locus | 第69-73页 |
| ·Evolutionarily conserved DNase Ⅰ hypersensitive sites within the promoter and intron regions of C13orf25 locus in B cell lymphoma DHL 16 and Granta 519 cells | 第73-75页 |
| ·Enhancer of the C13orf25 locus | 第75-78页 |
| ·E-box motifs function in the C13orf25 locus | 第78-83页 |
| ·Discussion | 第83-91页 |
| ·The miRNAs in the miR-17-92 cluster is regulated at several levels | 第83-84页 |
| ·The SP1 sites may be important in promoting cooperative recruitment of SP1 along with other transcription factors that bind the miR-17-92 cluster promoter | 第84-85页 |
| ·The relationship between the Histone Modifications in the C13orf25 Locus and C-MYC | 第85-86页 |
| ·Histone modifications involved in the C13orf25 gene regulation in B-Cell Malignancies | 第86-87页 |
| ·The C13orf25 gene is bound not only by MYC but also by inhibitory members of the MYC family | 第87-91页 |
| Conclusion | 第91-92页 |
| Acknowledgements | 第92-93页 |
| References | 第93-103页 |
| Appendix | 第103-144页 |
