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Studies on Susceptibility Genes and Functional Variants for Smoking Dependence by Using Molecular Biolgy and Bioinformatics Approaches

Chapter 1. Background and Literature Review第9-20页
    1.1. Genetic epidemiological of tobacco smoking第10页
    1.2. Neurobiology underlying nicotine addiction第10-12页
    1.3. Genetic approaches to complex traits第12-16页
    1.4. Previous association studies on smoking behavior第16-19页
    1.5. Molecular studies on functional SNPs and genes associated with smoking dependence第19-20页
Chapter 2. Serotonin Transporter and Receptor Genes Significantly Impact Nicotine Dependencethrough Genetic Interactions in both European American and African American Smokers第20-44页
    2.1 Abstract第21-22页
    2.2. Introduction第22-24页
    2.3 Materials and Methods第24-27页
        2.3.1 Subjects and ND Measures第24-25页
        2.3.2 DNA Sample Processing, and Selection and Genotyping of Polymorphic Variants第25-26页
        2.3.3 Statistical Analysis第26-27页
    2.4 Results第27-30页
        2.4.1 Quality control and pair-wise LD tests第27-28页
        2.4.2 Individual SNP-Based Association Analysis第28页
        2.4.3 Haplotype-Based Association Analysis第28-29页
        2.4.4 Gene-by-Gene Interaction Analysis of HTR3A, HTR3B, and SLC6A4第29-30页
    2.5 Discussion第30-44页
Chapter 3. Allelic Effects of SNP rs 1880676 of Choline Acetyltransferase on GeneExpression in HeLa Cells第44-62页
    3.1. Abstract第45-46页
    3.2. Introduction第46-47页
    3.3. Materials and Methods第47-53页
        3.3.1 Plasmid construction and mutagenesis第47-50页
        3.3.2 Plasmid amplification第50-51页
        3.3.3 Cell culture and transfection第51页
        3.3.4 mRNA expression level analysis第51-52页
        3.3.5 Western blotting analysis第52页
        3.3.6 Enzyme-linked immunoabsorbent assay (ELISA) analysis第52-53页
        3.3.7 Statistical analysis第53页
    3.4. Results第53-55页
        3.4.1 Comparison of ChAT mRNA expression of A and G alleles of rs1880676 in HeLa cells第53-54页
        3.4.2 Comparison of ChAT level of A and G allele of rs1880676 in HeLa cells第54页
        3.4.3 Comparison of ChAT concentrations of A and G allele of rs1880676 in HeLa cells第54-55页
    3.5. Discussion第55-62页
Chapter 4. Summary of Results and Future Research Directions第62-66页
    4.1. Summary of Results第63-64页
    4.2. Future research direction第64-66页
Chapter 5. List of References第66-90页
Chapter 6. Appendixes第90-91页
Acknowledgements第91页

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