| Abstract | 第4-5页 |
| Introduction | 第11-15页 |
| Chapter 1 Literature Review | 第15-33页 |
| 1.1 Pathomechanisms and histopathology changes in spinal cord injury | 第15-17页 |
| 1.2 Strategies for the repair of spinal cord injury | 第17-20页 |
| 1.2.1 Spinal cord injury models | 第18-19页 |
| 1.2.2 Implantation strategies and axon regeneration after spinal cord injury | 第19-20页 |
| 1.3 Neurotrophins factors (NTs) and their receptors | 第20-23页 |
| 1.3.1 Brain- derived neurotrophic factor(BDNF)and TrkB | 第22-23页 |
| 1.3.2 Neurotrophic-3 (NT-3) and TrkC | 第23页 |
| 1.4 Cell therapies | 第23-26页 |
| 1.5 Tissue engineering scaffold | 第26-30页 |
| 1.5.1 Spinal cord tissue engineering | 第28-29页 |
| 1.5.2 Acellular muscle scaffold | 第29-30页 |
| 1.6 Extracellular matrix (ECM) and axonal regeneration | 第30-33页 |
| 1.6.1 Fibronectin (FN) | 第31页 |
| 1.6.2 Laminin (LN) | 第31页 |
| 1.6.3 Neural cell adhesion molecules (N-CAM) | 第31-33页 |
| Chapter 2 repair mechanism of rat spinal cord injury byimplantation of chemically extracted acellular muscle scaffold | 第33-51页 |
| 2.1 Material | 第34-35页 |
| 2.1.1 Animals | 第34页 |
| 2.1.2 Instruments | 第34页 |
| 2.1.3 Reagents | 第34-35页 |
| 2.2 Methods | 第35-40页 |
| 2.2.1 Preparation of acellular muscle scaffolds | 第35-36页 |
| 2.2.2 Spinal cord hemiscection | 第36页 |
| 2.2.3 Transplantation of the acellular muscles scaffold in spinal cord hemisection | 第36-37页 |
| 2.2.4 Tissue processing | 第37页 |
| 2.2.5 Paraffin embedding, sectioning, and H&E staining | 第37-38页 |
| 2.2.6 Immunohistochemistry | 第38-39页 |
| 2.2.7 Holmes' silver staining of nerve fiber | 第39-40页 |
| 2.2.8 Image processing | 第40页 |
| 2.2.9 Statistical analysis | 第40页 |
| 2.3 Results | 第40-42页 |
| 2.3.1 Chemically extracted acellular muscle scaffold grafts | 第40页 |
| 2.3.2 Chemically extracted acellular muscle scaffold after implantaed in Lateral hemisection spinal cord | 第40-41页 |
| 2.3.3 Fibronectin and laminin detected in chemically extracted acellular muscle scaffold graft (before implantation) | 第41页 |
| 2.3.4 Neural adhesion molecules, fibronectin, laminin and S 100 proteins immuno-reactivity were detected in chemically acellular muscle scaffold graft at 7th day after implantation | 第41-42页 |
| 2.3.5 Sprouting fibers after implantation of acellular muscle scaffold | 第42页 |
| 2.4 Discussion | 第42-46页 |
| 2.4.1 Fibronectin and laminin expression in the chemically extracted acellular muscle scaffold | 第43页 |
| 2.4.2 The role of neural cell adhesions molecules, fibronectin, laminin, and S100 in spinal cord repair after chemically acellular muscle scaffold graft implantation | 第43-45页 |
| 2.4.3 Axonal regeneration through chemically acellular muscle scaffold grafts | 第45-46页 |
| 2.5 Summary | 第46页 |
| 2.6 Appendix | 第46-51页 |
| Chapter 3 repair mechanism of rat spinal cord injury byimplantation of chemically extracted acellular muscle scaffold seededwith amniotic epithelial cells | 第51-71页 |
| 3.1 Amniotic epithelial cell culture | 第52-53页 |
| 3.1.1 Animals | 第52页 |
| 3.1.2 Instruments | 第52页 |
| 3.1.3 Reagents | 第52-53页 |
| 3.2 Methods | 第53-58页 |
| 3.2.1 Isolation and culture ofrat AECs | 第53-54页 |
| 3.2.2 Preparation of graft and experimental animal grouping | 第54页 |
| 3.2.3 Immunohistochemistry | 第54-55页 |
| 3.2.4 Immunofluorescence | 第55-56页 |
| 3.2.5 Toluidine blue staining of axons myelination | 第56页 |
| 3.2.6 Electron microscope observation of axons myelination | 第56-57页 |
| 3.2.7 Image processing | 第57-58页 |
| 3.2.8 Statistical analysis | 第58页 |
| 3.3 Results | 第58-60页 |
| 3.3.1 AECs- acellular muscles scaffold graft (H&E staining) | 第58页 |
| 3.3.2 BDNF and NT-3 immunoreactivity | 第58-59页 |
| 3.3.3 TrkB and TrkC immunoreactivity | 第59页 |
| 3.3.4 NF immunofluorescent staining for nerve sprouting | 第59页 |
| 3.3.5 Toluidine blue staining for Axonal remyelination | 第59-60页 |
| 3.4 Discussion | 第60-63页 |
| 3.5 Summary | 第63-64页 |
| 3.6 Appendix | 第64-71页 |
| Conclusions | 第71-73页 |
| Innovation | 第73-75页 |
| References | 第75-97页 |
| Acknowledgements | 第97页 |
