| 摘要 | 第1-6页 |
| ABSTRACT | 第6-11页 |
| 1 IntrOdlietion | 第11-13页 |
| 2 Background | 第13-20页 |
| ·History of miRNA | 第13-14页 |
| ·Biogenesis of miRNAs | 第14页 |
| ·Functional characterization of miRNAs | 第14-16页 |
| ·Prediction targeted miRNAs of genes | 第16-17页 |
| ·Human polymorohism at microRNAs and micrORNA target sites as a new mechanism for tumorigenesis | 第17-20页 |
| 3 Chapter Ⅰ | 第20-39页 |
| ·ColJection analysis of genes involVed in the pathway of tumorigenesis | 第20-22页 |
| ·Nasopharyngeal carcinoma etiology and epidemiolOgy | 第20-21页 |
| ·The molecular mechanisms of nasopharyngeal careinoma | 第21-22页 |
| ·Drawing genes network involved the seven hallmarks in the NPC | 第22-30页 |
| ·self-sufficiency in growth signals | 第22-24页 |
| ·insensitiVity to growth signals | 第24-26页 |
| ·Evasion of apoptosis | 第26-27页 |
| ·Limitless replicative potential | 第27-28页 |
| ·Sustained angiogenesis | 第28页 |
| ·Tissue invasioil or metastasis | 第28-29页 |
| ·Cancer-related innammation | 第29-30页 |
| ·Metabolism-related genes | 第30页 |
| ·Building genes networks implicated the seven hallmarks in NPC | 第30页 |
| ·Predicted polymorphic miRNA binding-sites by bioinformation | 第30-34页 |
| ·Analysis of Candidate genes | 第30-31页 |
| ·To downIoad candidate genes 3'UTR sequences | 第31-32页 |
| ·Finding the 3'UTR polymorphisms of candidate genes | 第32页 |
| ·Computational predictions of target miRNA | 第32页 |
| ·Finding polymorphic miRNA binding-sites | 第32页 |
| ·Assessment the binding free energy | 第32页 |
| ·Selection the SNPs and genes | 第32-34页 |
| Discrss | 第34-39页 |
| 4Chapter Ⅱ | 第39-55页 |
| ·Laboratory techniques | 第39-44页 |
| ·Study population and epidemiologic data | 第39-41页 |
| ·DNA isolatiOn from EDTA blood | 第41页 |
| ·Primer design | 第41-42页 |
| ·Polymerase chain reaction analysis of polymorphisms | 第42-43页 |
| ·Agarose gel eIectrophoresis | 第43页 |
| ·DNA sequencing of PCR products | 第43-44页 |
| ·Statistical data analysis | 第44页 |
| ·Screening for functioilal sequence vatiations in candidate genes | 第44-45页 |
| ·High-thrOUghput genotyping of candidate polymorphsims | 第45-52页 |
| ·Genotype distribution in MMP2 rs7201 and EGFR rs884225 of cases and Controls,and their association with the risk of nasopharyngeal carcinoma | 第45-48页 |
| ·PolymorPhism in the CCNEI rs3218073 as a risk factor for nasopharyngeal carcinoma | 第48-49页 |
| ·Prognostic significance of polymorphism(rs3218073)in the CCNE1 gene | 第49-52页 |
| DiSCUSS | 第52-55页 |
| Reference | 第55-65页 |
| Acknowledgements | 第65-66页 |
