| Abstract in Chinese | 第1-7页 |
| Abstract | 第7-12页 |
| Abbreviations | 第12-14页 |
| Introduction | 第14-36页 |
| 1) Materials and methods | 第15-19页 |
| ·) Experimental animal | 第15页 |
| ·) Environment for mice | 第15页 |
| ·) Reagents | 第15-16页 |
| ·) Preparat ion of experimental solution and buffer | 第16-18页 |
| ·) Equipments | 第18-19页 |
| 2) Set up the acute asthma model of mouse | 第19-24页 |
| ·) Ovalbumin sensitization and airway challenge | 第19-20页 |
| ·) Pulmonary Function Test (PFT) | 第20-21页 |
| ·) BALF withdraw, cells count and classification | 第21-23页 |
| ·) Lung histology | 第23-24页 |
| 3) Separation and purification of CD4~+ T lymphocytes isolated from the spleen of acute asthmatic and normal group mice | 第24-26页 |
| 4) Culture of isolated CD4~+ T cells for 24 hours | 第26页 |
| 5) Determination of Th 17 cells by Flow Cytometry (FCM) in acute asthmatic and normal group | 第26-27页 |
| 6) Determination of IL-17 in BALF and CD4~+ T-lymphocytes culture supernatant by ELISA | 第27-28页 |
| 7) Preparation and addition of Rosiglitazone and Dexamethasone in respective groups sample | 第28-29页 |
| 8) CD4~+ T-lymphocytes culture after the addition of rosiglitazone and dexamethasone in Aros and Adex group | 第29页 |
| 9) Western Blot to detect the IL-17 protein expression from CD4~+ T-lymphocytes after 24 hours of culture from all the groups | 第29-32页 |
| 10) real- time Polymerase Chain Reaction (rt-PCR) to detect the expression of IL-17 mRNA from CD4~+ T-lymphocytes after 24 hours of culture | 第32-35页 |
| 11) Statistical Analysis | 第35-36页 |
| Results | 第36-52页 |
| 1) Confirmation of acute asthma model | 第36-41页 |
| 2) Concentration of isolated CD4~+ T-lymphocytes in normal and acute asthmatic group | 第41-42页 |
| 3) Viability of isolated CD4~+ T-lymphocytes after 24 hours of culture | 第42-43页 |
| 4) Th 17 cells level in CD4~+ T-lymphocytes in acute asthmatic and normal group | 第43-44页 |
| 5) Concentration of IL-17 in HALF and CD4~+ T lymphocytes culture supernatant in normal and asthmatic group | 第44-46页 |
| 6) CD4~+ T-lymphocytes viability 24 hours after the addition addition of rosiglitazone and dexamethasone | 第46-47页 |
| 7) Western Blot detection of IL-17 protein expression in CD4~+ T-lymphocytes after 24 hours of culture from all the groups | 第47-49页 |
| 8) Expression of IL-17 mRNA by real-time Polymerase Chain Reaction (rt - PCR) from CD4~+ T-lymphocytes after 24 hours of culture in all the groups | 第49-52页 |
| Discussion | 第52-57页 |
| Conclusion | 第57-58页 |
| Refrences | 第58-63页 |
| Review | 第63-79页 |
| ACKNOWLEDGEMENTS | 第79-80页 |
| ACADEMIC ACHIEVEMENTS | 第80页 |
