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Creating and Evaluation of New-Type Brassica Juncea By Backcrossing with Hexaploid Derived from B.Rapa and B. Carinata

摘要第6-7页
ABSTRACT第7-9页
ABBREVIATIONS第10-11页
1. INTRODUCTION & REVIEW OF LITERATURE第11-30页
    1.1 General introduction to genus Brassica species第11页
    1.2. Origin, Taxonomy and Distribution of Brassica species第11-14页
        1.2.1 Diploid Species第11-13页
        1.2.2 Tetrapod Species第13-14页
    1.3 Genome evolution in Brassica species第14-15页
    1.4 Current cultivation and utilization of Brassica species worldwide第15-17页
    1.5 The genomic differentiation among different Brassica species第17-21页
    1.6 Novel Brassica germplasm development by using introgressing sub-genomic differentiation第21-29页
    1.7 Aim and an overview of the present study第29-30页
        1.7.1 Aim of the study第29页
        1.7.2 An overview of the present study第29-30页
2. MATERIALS AND METHODS第30-40页
    2.1 Plant materials第30-32页
    2.2 Agronomic Traits第32页
    2.3 Seed quality analysis第32页
    2.4 Cytological observation第32-33页
    2.5 Genotype assays by SSR第33-39页
        2.5.1 DNA extraction第34-35页
        2.5.2 Chemical recipes第35-36页
        2.5.3 DNA quantity and quality estimation第36页
        2.5.4 PCR thermo profile and amplification mixture第36-37页
        2.5.5 DNA Page第37-39页
    2.6 Exploitation of heterosis in intersubgenomic hybirds between new-type B. juncea and traditional B. juncea第39页
    2.7 Statistical analysis第39-40页
3. RESULTS第40-58页
    3.1 Genetic variability for agronomic traits in BC_1F_3 and BC_1F_4 compared with traditional B.juncea第40-44页
        3.1.1 Plant height第41页
        3.1.2 Days taken to flower第41页
        3.1.3 Branches plant~(-1)第41页
        3.1.4 Pod length(cm)第41-42页
        3.1.5 1000-Seed Weight(g)第42页
        3.1.6 Yield plant~(-1) (g)第42-44页
    3.2 Variability for quality traits in BC_1F_3 and BC_1F_4第44-47页
        3.2.1 Protein Content(%)第44页
        3.2.2 Oil Content(%)第44页
        3.2.4 Moisture content(%)第44页
        3.2.5 Linolenic acid(%)第44-45页
        3.2.6 Oleic acid(%)第45-47页
    3.3 Correlation coefficients(r)studies第47-50页
        3.3.1 Results obtained by phenotypic correlation in BC_1F_3第47-50页
    3.4 Seed setting rate第50-51页
    3.5 Pollen fertility第51-53页
    3.6 Genetic variation among BC_1F_4 population and their parents第53-55页
    3.7 Intersubgenommic heterosis of the hybrids between traditional B.juncea and new-type B.juncea第55-58页
4. DISCUSSION第58-62页
REFERENCES第62-68页
SUPPLEMENTARY DATA第68-76页
DEDICATION第76-77页
ACKNOWLEDGEMENTS第77-78页

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