| Acknowledgements | 第7-13页 |
| Abbreviations | 第13-14页 |
| Abstract | 第14-15页 |
| 摘要 | 第16-17页 |
| CHAPTER 1 Literature review | 第17-23页 |
| 1.1 Background | 第17页 |
| 1.2 Cadmium and copper related environmental threats | 第17-18页 |
| 1.3 Contrasting proprieties of Cd and Cu | 第18-19页 |
| 1.4 General path of Cd and Cu toxicity in plant | 第19-20页 |
| 1.5 Coexistence of Cd and Cu in contaminated media and toxicological implications of their interactive effects | 第20-21页 |
| 1.6 Cd and Cu detoxifying responses of plant | 第21-22页 |
| 1.7 Research objectives | 第22-23页 |
| CHAPTER 2 Individual and interactive toxicity of cadmium and copper regarding accumulation and physio-chemicalattributes in rapeseed | 第23-45页 |
| 2.1 Introduction | 第23-24页 |
| 2.2 Materials and methods | 第24-27页 |
| 2.2.1 Plant materials and experimental conditions | 第24页 |
| 2.2.2 Assessment of morphological changes and elements uptake | 第24-25页 |
| 2.2.3 Chlorophyll measurement | 第25页 |
| 2.2.4 Determination of indices of oxidative stress | 第25-26页 |
| 2.2.5 Thiols compounds measurements and GR assays | 第26-27页 |
| 2.2.6 Assessment of metals interactive effect on plant growth and physiological attributes | 第27页 |
| 2.2.7 Statistical analysis | 第27页 |
| 2.3 Results | 第27-39页 |
| 2.3.1 Cd and Cu uptake and distribution in plants | 第27-30页 |
| 2.3.2 Plant dry biomass and chlorophyll content | 第30-32页 |
| 2.3.3 MDA and reactive oxygen species (ROS) accumulation in plant tissues | 第32-34页 |
| 2.3.4 Nutrients contents | 第34-37页 |
| 2.3.5 Cd and Cu effects on the GSH pathway | 第37-39页 |
| 2.4 Discussion | 第39-45页 |
| 2.4.1 Differential Cd and Cu toxic effects on B. napus plants | 第39-41页 |
| 2.4.2 Cd and Cu interactive effects | 第41-45页 |
| CHAPTER 3 Comparative subcellular distribution and chemical form of Cd and Cu in oilseed rape | 第45-63页 |
| 3.1 Introduction | 第45-46页 |
| 3.2 Materials and methods | 第46-48页 |
| 3.2.1 Plant material and experimental conditions | 第46页 |
| 3.2.2 Plants tissues fractionation and metal analysis | 第46-47页 |
| 3.2.3 Analysis of metals chemical form | 第47页 |
| 3.2.4 Transmission electron microscopy | 第47-48页 |
| 3.2.5 Statistical analysis | 第48页 |
| 3.3 Results | 第48-59页 |
| 3.3.1 Subcellular distribution | 第48-55页 |
| 3.3.2 Chemical form | 第55-59页 |
| 3.4 Discussion | 第59-63页 |
| CHAPTER 4 General conclusions and perspectives | 第63-65页 |
| References | 第65-71页 |
