| 摘要 | 第1-9页 |
| ABSTRACT | 第9-13页 |
| FIGURES | 第13-15页 |
| TABLES | 第15-16页 |
| CHAPTER 1 INTRODUCTION | 第16-21页 |
| §1.1 Previous Study of Heavy Metal on Soil Microorganisms | 第16页 |
| §1.2 Introduce of Heavy Metal | 第16-17页 |
| ·Feature of soil Pollution by Heavy Metal Cd(Ⅱ) | 第17页 |
| ·Actuality of Contaminated Soil by Cd(Ⅱ) | 第17页 |
| §1.3 Bioremediation Study of Contaminated Soil by Cd(Ⅱ) | 第17-19页 |
| ·Method of Bioremediation on Contaminated Soil | 第18页 |
| ·Bioremediation Study of Contaminated Soil by Cd(Ⅱ) | 第18-19页 |
| §1.4 Microcalorimetric Method Study Mechanism of Strain | 第19页 |
| ·Microcalorimetric Method Development and Significance | 第19页 |
| ·Application of Microcalorimetric Method on Bioremediation of Cd(Ⅱ) Contaminated Soil | 第19页 |
| §1.5 Objectives and Significance of This Study | 第19-21页 |
| CHAPTER 2 AIM STRAIN STUDY | 第21-25页 |
| §2.1 Significance to Study and Isolated Candida humicola and Bacillus subtili | 第21-24页 |
| §2.2 Significance in Study Cadmium-Resistant | 第24-25页 |
| CHAPTER 3 INTRODUCTION O FCD(Ⅱ) | 第25-27页 |
| §3.1 Study on Mechanism of Cadmium-Resistant Strain | 第25-26页 |
| §3.2 Significance to Contaminated Soil Bioremediation | 第26-27页 |
| CHAPTER 4 MATERIALS AND METHODS | 第27-31页 |
| §4.1 Materials Source and Strain Prepare | 第27页 |
| ·Candida humicola | 第27页 |
| ·Bacillus subtilis | 第27页 |
| ·Cd(Ⅱ) | 第27页 |
| §4.2 Methods and Analysis Test | 第27-31页 |
| ·Microcalorimetric Method | 第28页 |
| ·Biosensor Method of Strain and Pretreatment | 第28-29页 |
| ·Instrument Analysis on Heavy Metal by Ultraviolet Spectrophotometer Method | 第29-30页 |
| ·Analysis Quality Control | 第30-31页 |
| CHAPTER 5 DATING BY THERMOGENIC CURVES | 第31-33页 |
| CHAPTER 6 RESISTIBILITY COMPARISON BETWEEN TWO STRAIN | 第33-49页 |
| §6.1 Different Resistibility to Heavy Metal Cd(Ⅱ) | 第33-39页 |
| ·Thermogenic and Biomass Curves of Strains | 第33-36页 |
| ·pH Values of Two Strains | 第36-38页 |
| ·Different Oxygen Demand(BOD) of Strains | 第38-39页 |
| §6.2 Analysis Experiments on Strainsfor Pure Microbes and Both | 第39-49页 |
| ·The Metabolic Regularity Externalized by Thermogenic Method | 第40-41页 |
| ·The Metabolic Regularity Externalized by Biosensor | 第41-43页 |
| ·OD600 Values Externalized by Ultraviolet Spectrophotometer Method | 第43-45页 |
| ·Different Parameters Relationship Between Strains | 第45-49页 |
| CHAPTER 7 DISCUSSION AND ANALYSIS | 第49-52页 |
| §7.1 Discussion | 第49页 |
| §7.2 Analysis | 第49-52页 |
| ·Thermogenic and Biomass Curves of each strain and Mixture | 第49-50页 |
| ·The Relationship among the Parameters of Microorganisms | 第50页 |
| ·The Analysis of Metabolic Regularity w Measured by Biosensor | 第50-51页 |
| ·The pH Curves Influenced by Different Concentration of Cd~(2+) | 第51-52页 |
| CHAPTER 8 CONCLUSIONS AND FU TURE PROSPECTS | 第52-60页 |
| §8.1 Conclusion | 第52-57页 |
| §8.2 Future Prospect | 第57-60页 |
| ACKNOWLEDGMENTS | 第60-61页 |
| REFERENCES | 第61-69页 |
| GLOSSARY AND NOMENCLATURE | 第69页 |