| 摘要 | 第6-8页 |
| Abstract | 第8-9页 |
| List of Symbols and Abbreviations | 第10-13页 |
| Table of Contents | 第13-16页 |
| List of Figures | 第16-20页 |
| CHAPTER 1 GENERAL INTRODUCTION | 第20-26页 |
| CHAPTER 2 Literature Review | 第26-38页 |
| 2.1 Cellular stress and Reactive oxygen species | 第27-29页 |
| 2.2 Parkinson’s disease (PD) | 第29-30页 |
| 2.3 Neurodegenerative Diseases and Autophagy | 第30-31页 |
| 2.4 Autophagy and Mitochondrial ROS/RNS | 第31-32页 |
| 2.5 Autophagy and Alpha Synuclein | 第32-33页 |
| 2.6 Characterization of BNIP3 | 第33-34页 |
| 2.7 Cellular localization of BNIP3 | 第34-36页 |
| 2.8 BNIP3 induce different types of cell death | 第36-38页 |
| CHAPTER 3 Materials and Methods | 第38-44页 |
| 3.1 Cell Culture | 第38页 |
| 3.2 mRNA extraction Trizol reagents | 第38-39页 |
| 3.3 RTPCR and PCR | 第39-40页 |
| 3.4 Gel Electrophoresis | 第40页 |
| 3.5 DNA purification | 第40页 |
| 3.6 Transfection | 第40-41页 |
| 3.7 Protein Extraction | 第41页 |
| 3.8 Bradford assay of protein | 第41页 |
| 3.9 Western Blot Analysis | 第41-42页 |
| 3.10 H_2O_2Treatment and Viability of Cell | 第42页 |
| 3.11 Flow Cytometry analysis | 第42页 |
| 3.12 Labeling of autophagic vacuoles with monodansylcadaverine (MDC) | 第42-43页 |
| 3.13 Hypoxia induced by CoCl_2 solution | 第43页 |
| 3.14 Cell viability assays | 第43页 |
| 3.15 statistic analysis | 第43-44页 |
| CHAPTER 4 Result and Discussion | 第44-88页 |
| 4.1 Neuroprotective Role of Bnip3 under Oxidative Stress through Autophagy In Neuroblastoma Cells | 第44-51页 |
| 4.1.1 Cellular Viability under Oxidative Stress | 第46-47页 |
| 4.1.2 Expression of Bnip3 under Oxidative Stress | 第47页 |
| 4.1.3 BNIP3 Induce Autophagy under Stress in Neuroblastoma Cells | 第47-49页 |
| 4.1.4 The Level of Lc3 Is Increased Under Oxidative Stress | 第49-51页 |
| 4.2 Bnip3 protects the Neural cells death from Alpha synuclein Toxicity through Autophagy | 第51-59页 |
| 4.2.1 Expression of Bnip3 under Alpha Synuclein Stress | 第52-54页 |
| 4.2.2 Bnip3 induces Autophagy to protect the cell death under the stress of alpha synuclein | 第54-55页 |
| 4.2.3 Bnip3 reduces the level of apoptosis | 第55-56页 |
| 4.2.4 Cell cycle arrest under alpha synuclein stress | 第56-57页 |
| 4.2.5 Hypoxia induced expression of Bnip338 | 第57-59页 |
| 4.3. Characterization of the expression of Bnip3 under oxidative stress in neuroblastoma cells | 第59-66页 |
| 4.3.1 Bnip3 express under oxidative stress | 第60-61页 |
| 4.3.2 Over Expression of Bnip3 causes Apoptotic cell death | 第61-62页 |
| 4.3.3 H2O2Induce cell death dose dependent | 第62-63页 |
| 4.3.4 Bnip3 under Oxidative Stress effect Cell cycle | 第63-66页 |
| 4.4 Effect of Hypoxia on the Efficiency of Bnip3 in Neuroblastoma Cells | 第66-72页 |
| 4.4.1 Bnip3 reduced Cocl2induced cell death | 第67-68页 |
| 4.4.2 Expression of Bnip3 acts as protectant under hypoxic condition | 第68-72页 |
| 4.5. Role of autophagy and its significance in cellular homeostasis | 第72-82页 |
| 4.5.1 Genetic liaison of autophagy | 第75-78页 |
| 4.5.2 Autophagy under stress | 第78-81页 |
| 4.5.3 ER and autophagy | 第81-82页 |
| 4.6. Discussion | 第82-88页 |
| REFERENCES | 第88-121页 |
| ACKNOWLEDGEMENT | 第121-123页 |
| List of Publications | 第123-125页 |
