| 摘要 | 第6-7页 |
| ABSTRACT | 第7页 |
| Chapter 1. A new sulfonic acid derivative, (Z)-4-methylundeca-1,9-diene-6-sulfonic acid, isolated from the cold water sea urchin inhibits inflammatory responses through JNK/p38 MAPK and NF-κB inactivation in RAW | 第11-30页 |
| INTRODUCTION | 第12-13页 |
| MATERIALS AND METHODS | 第13-18页 |
| 1. Materials | 第13-14页 |
| 1.1 Marine natural product materials | 第13页 |
| 1.2 Reagents and instruments for isolation of compounds | 第13页 |
| 1.2.1 Reagents | 第13页 |
| 1.2.2 Instruments | 第13页 |
| 1.3 Reagents and instruments for bioassay | 第13-14页 |
| 1.3.1 Reagents | 第13-14页 |
| 1.3.2 Instruments | 第14页 |
| 2. Methods | 第14-18页 |
| 2.1 Extraction and isolation of the sea urchin Brisaster latifrons (09C-ST8-DR2) | 第14-15页 |
| 2.2 Cell culture and viability assay | 第15页 |
| 2.3 Measurement of NO production | 第15-16页 |
| 2.4 PGE2, TNF-α, and IL-1β assays | 第16页 |
| 2.5 Preparation of cytosolic and nuclear fractions | 第16-17页 |
| 2.6 Western blot analysis | 第17页 |
| 2.7 DNA-binding activity of NF-κB | 第17页 |
| 2.8 Statistical analysis | 第17-18页 |
| RESULTS | 第18-25页 |
| 1. Structure elucidation of isolated compound | 第18-19页 |
| 2. Effects of compound 1 on cell viability in RAW264.7 macrophages | 第19-20页 |
| 3. Effects of compound 1 on the production of pro-inflammatory mediators and cytokines in RAW264.7 macrophages stimulated with LPS | 第20-21页 |
| 4. Effects of compound 1 on the expression of iNOS and COX-2 proteins in LPS-stimulated RAW264.7 macrophages | 第21-22页 |
| 5. Effects of compound 1 on IκB-levels, NF-κB nuclear translocation, and DNA-binding activity of NF-κB in LPS-stimulated RAW264.7 macrophages | 第22-23页 |
| 6. Effects of compound 1 on the phosphorylation of MAPKs in LPS-stimulated RAW264. 7 macrophages | 第23-25页 |
| DISCCUSION | 第25-28页 |
| REFERENCES | 第28-30页 |
| Chapter 2. Anti-inflammatory effect of neoechinulin A from the marine fungus Eurotium sp. SF-5989 through the suppression of NF-kB and p38 MAPK pathways in lipopolysaccharide-stimulated RAW264.7 macrophages | 第30-50页 |
| INTRODUCTION | 第31-33页 |
| MATERIALS AND METHODS | 第33-38页 |
| 1. Materials | 第33-34页 |
| 1.1 Marine natural product materials | 第33页 |
| 1.2 Reagents and instruments for isolation of compounds | 第33页 |
| 1.2.1 Reagents | 第33页 |
| 1.2.2 Instruments | 第33页 |
| 1.3 Reagents and instruments for bioassay | 第33-34页 |
| 1.3.1 Reagents | 第34页 |
| 1.3.2 Instruments | 第34页 |
| 2. Methods | 第34-38页 |
| 2.1 Specimen collection and identification of the marine-derived fungus Eurotium sp.SF-5989 | 第34页 |
| 2.2 Fermentation, extraction and isolation of neoechinulins A (2) and B (3) from Eurotium sp. SF-5989 | 第34-35页 |
| 2.3 Cell culture and viability assay | 第35-36页 |
| 2.4 Determination of nitrite production and PGE2,TNF-α,and IL-1β assays | 第36页 |
| 2.5 Preparation of cytosolic and nuclear fractions | 第36页 |
| 2.6 Western blot analysis | 第36-37页 |
| 2.7 DNA-binding activity of NF-κB | 第37页 |
| 2.8 Immunofluorescence | 第37页 |
| 2.9 Statistical analysis | 第37-38页 |
| RESULTS AND DISCUSSION | 第38-45页 |
| 1. Identification of neoechinulins A(2)and B(3) | 第38-39页 |
| 2. Effects of neoechinulins A(2)and B(3)on cell viability and pro-inflammatory mediators/cytokines production | 第39-41页 |
| 3. Effect of neoechinulin A(2)on iNOS and COX-2 expression | 第41-42页 |
| 4. Effect of neoechinulin A(2)on NF-κB activation | 第42-43页 |
| 5. Effect of neoechinulin A(2)on the phosphorylation of MAPKs | 第43-45页 |
| CONCLUSIONS | 第45-46页 |
| REFERENCES | 第46-50页 |
| 致谢 | 第50-51页 |
| APPENDIX(1) | 第51-52页 |
| APPENDIX(2) | 第52-53页 |
| 附图 | 第53-65页 |
