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Physiological and Genetic Characterization of a Maize Dwarf Mutant

摘要第3-5页
ABSTRACT第5-6页
ABBREVITION第11-13页
CHAPTER 1 GENERAL INTRODUCTION第13-22页
    1.1 Phenotype of Dwarf mutants第13-16页
        1.1.1 Plant height in dwarf mutants第14页
        1.1.2 Internodes distance in dwarf mutants第14-15页
        1.1.3 Size of leaves of dwarfing mutant第15-16页
        1.1.4 Cell size of dwarfing mutant第16页
    1.2 Physiological basis of dwarf mutants第16-18页
        1.2.1 GA-sensitive dwarf mutants第17页
        1.2.2 GA-insensitive dwarf mutants第17-18页
    1.3 Molecuar basis of dwarf mutants第18-20页
        1.3.1 Mapping of dwarfing genes第18-19页
        1.3.2 Cloning of dwarfing genes第19-20页
    1.4 Ojectives of this study第20-22页
CHAPTER 2 PHENOTYPIC CHARACTERIZATION OF A MAIZEDWARF MUTANT第22-40页
    2.1 INTRODUCTION第22-24页
    2.2 MATERIAL AND METHODS第24-26页
        2.2.1 Plant Material第24页
        2.2.2 Expermmental design第24-25页
        2.2.3 GAs treatments第25页
        2.2.4 Cell size measuremants第25-26页
        2.2.5 Statistical analysis第26页
    2.3 RESULTS第26-37页
        2.3.1 Overview of dwarf mutant M34第26-27页
        2.3.2 Plant height of dwarf mutant M34第27-28页
        2.3.3 Intemodes distance of dwarf mutant M34第28-30页
        2.3.4 Leaf size of dwarf mutant M34第30-32页
        2.3.5 GA sensitivity of dwarf mutant M34第32-33页
        2.3.6 Gibberellin(GA)sensitivity on trumpet of dwarf mutant M34第33-34页
        2.3.7 Gibberellin(GA)sensitivity on internodes length of dwarf mutant M34第34-35页
        2.3.8 Gibberellin(GA)on number of leaves of dwarf mutant M34第35-36页
        2.3.9 Measurement of Cell size of dwarf mutan t M34第36-37页
    2.4 DISCUSSION第37-40页
CHAPTER 3 MAP-BASED CLONING OF DWARFING GENE第40-51页
    3.1 INTRODUCTION第40-41页
    3.2 MATERIALAND MATHODS第41-45页
        3.2.1 Plant material第41页
        3.2.2 Genetic analysis第41-42页
        3.2.3 DNA amplification,PAGE analysis第42页
        3.2.4 Constructions of linkage map第42-43页
        3.2.5 Bulk segregation analysis第43页
        3.2.6 Analysis of SSR markers for candidate gene第43-44页
        3.2.7 Cloning and sequencing analysis第44-45页
    3.3 RESULTS第45-49页
        3.3.1 Genetic analysis of dwarf mutant M34第45页
        3.3.2 Preliminary-mapping of dwarf mutant M34第45-46页
        3.3.3 Fine-mapping of dwarf mutant M34第46页
        3.3.4 Analysis of SSR markers for dwarf mutant M34第46-47页
        3.3.5 Cloning and sequencing of dwarf mutant M34第47-49页
    3.4 DISCUSSION第49-51页
SUMMARY第51-53页
REFERENCES第53-64页
ACKNOWLEDGEMENT第64-65页
CURRICULUM VITAE第65页

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